Self-sufficient cytochrome P450 monooxygenases from Bacillus thuringiensis: amplification and comparative sequence analysis

Biologija ◽  
2008 ◽  
Vol 54 (3) ◽  
pp. 187-191
Author(s):  
Romualdas Griškevičius ◽  
Nomeda Kuisienė ◽  
Juozas Raugalas ◽  
Džiuginta Jasinskytė ◽  
Donaldas Čitavičius
Keyword(s):  
Cytochrome P450 ◽  
Sequence Analysis ◽  
Bacillus Thuringiensis ◽  
Comparative Sequence Analysis ◽  
Cytochrome P450 Monooxygenases ◽  
Comparative Sequence ◽  
P450 Monooxygenases

Comparative sequence analysis of rotavirus genomic segment 6--the gene specifying viral subgroups 1 and 2.

1984 ◽  
Vol 51 (1) ◽  
pp. 97-101 ◽  
Author(s):  
G W Both ◽  
L J Siegman ◽  
A R Bellamy ◽  
N Ikegami ◽  
A J Shatkin ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Comparative Sequence Analysis ◽  
Genomic Segment ◽  
Comparative Sequence

Characterization of cytochrome P450-monooxygenases of Chinese hamsters with respect to aflatoxin B1 activation

Toxicology ◽  
1994 ◽  
Vol 93 (2-3) ◽  
pp. 165-173 ◽  
Author(s):  
Morio Fukuhara ◽  
Eric Antignac ◽  
Naomi Fukusen ◽  
Kazue Kato ◽  
Masanobu Kimura
Keyword(s):  
Cytochrome P450 ◽  
Aflatoxin B1 ◽  
Cytochrome P450 Monooxygenases ◽  
P450 Monooxygenases ◽  
Chinese Hamsters

scholarly journals Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178829 ◽  
Author(s):  
Wander de Jesus Jeremias ◽  
Flávio Marcos Gomes Araújo ◽  
Fábio Ribeiro Queiroz ◽  
Fabiano Sviatopolk Mirsky Pais ◽  
Ana Carolina Alves de Mattos ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Schistosoma Mansoni ◽  
Comparative Sequence Analysis ◽  
Comparative Sequence

scholarly journals Primary structure and comparative sequence analysis of an insect apolipoprotein. Apolipophorin-III from Manduca sexta.

1987 ◽  
Vol 262 (24) ◽  
pp. 11794-11800 ◽  
Author(s):  
K D Cole ◽  
G P Fernando-Warnakulasuriya ◽  
M S Boguski ◽  
M Freeman ◽  
J I Gordon ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Manduca Sexta ◽  
Primary Structure ◽  
Comparative Sequence Analysis ◽  
Comparative Sequence ◽  
Apolipophorin Iii

scholarly journals Specificity and mechanism of carbohydrate demethylation by cytochrome P450 monooxygenases

2018 ◽  
Vol 475 (23) ◽  
pp. 3875-3886 ◽  
Author(s):  
Craig S. Robb ◽  
Lukas Reisky ◽  
Uwe T. Bornscheuer ◽  
Jan-Hendrik Hehemann
Keyword(s):  
Cytochrome P450 ◽  
Active Site ◽  
Substrate Recognition ◽  
High Energy ◽  
Cytochrome P450 Monooxygenases ◽  
P450 Monooxygenases ◽  
High Energy Barrier ◽  
Molecular Determinants ◽  
Cytochrome P450 Family ◽  
Carbohydrate Ligand

Degradation of carbohydrates by bacteria represents a key step in energy metabolism that can be inhibited by methylated sugars. Removal of methyl groups, which is critical for further processing, poses a biocatalytic challenge because enzymes need to overcome a high energy barrier. Our structural and computational analysis revealed how a member of the cytochrome P450 family evolved to oxidize a carbohydrate ligand. Using structural biology, we ascertained the molecular determinants of substrate specificity and revealed a highly specialized active site complementary to the substrate chemistry. Invariance of the residues involved in substrate recognition across the subfamily suggests that they are critical for enzyme function and when mutated, the enzyme lost substrate recognition. The structure of a carbohydrate-active P450 adds mechanistic insight into monooxygenase action on a methylated monosaccharide and reveals the broad conservation of the active site machinery across the subfamily.

scholarly journals Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes

2004 ◽  
Vol 135 (1) ◽  
pp. 459-470 ◽  
Author(s):  
Yong Qiang Gu ◽  
Devin Coleman-Derr ◽  
Xiuying Kong ◽  
Olin D. Anderson
Keyword(s):  
Sequence Analysis ◽  
Genome Evolution ◽  
Comparative Sequence Analysis ◽  
Comparative Sequence
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