scholarly journals The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro Study

2020 ◽  
Vol 29 (1) ◽  
pp. 1-8
Author(s):  
Hamid Hammad Enezei ◽  
Ali Al Qabbani ◽  
Azlina Ahmad ◽  
Mohd Fadhli Khamis ◽  
Abdelkader Hassani ◽  
...  
2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Casiano Del Angel-Mosqueda ◽  
Yolanda Gutiérrez-Puente ◽  
Ada Pricila López-Lozano ◽  
Ricardo Emmanuel Romero-Zavaleta ◽  
Andrés Mendiola-Jiménez ◽  
...  

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 255-258
Author(s):  
Faisal A. Alzahrani

SummaryThis study aimed to optimize the derivation of trophectoderm from in vitro-produced camel embryos under feeder-free culture conditions using the basement membrane matrix Matrigel. Trophoblastic vesicles were obtained through mechanical microdissection of in vitro-produced camel (Camelus dromedarius) embryos. Supplementing the culture medium with 10 ng/ml of epidermal growth factor and 10 ng/ml fibroblast growth factor improved the attachment and subsequent outgrowths of cultured trophoblastic vesicles when compared with the control group and the groups supplemented individually with each growth factor. The expression levels of pluripotency genes octamer-binding transcription factor 4 (Oct4), sex determining region Y-box 2 (Sox2), myelocytomatosis proto-oncogene (c-Myc) and anti-apoptotic gene B-cell lymphoma 2 (Bcl2) were increased in trophoblastic vesicles supplemented with both growth factors when compared with the control group. Conversely, both growth factors decreased the expression of apoptotic genes tumour protein p53 (p53) and Bcl-2-associated X protein (Bax). To the best of our knowledge, this may be the first report describing the derivation of trophoblast stem cells from in vitro-produced camel embryos.


2018 ◽  
Vol 24 ◽  
pp. 4254-4261 ◽  
Author(s):  
Shangliang Chen ◽  
Mingzhu Wang ◽  
Xinglu Chen ◽  
Shaolian Chen ◽  
Li Liu ◽  
...  

2012 ◽  
Vol 131 (5) ◽  
pp. E791-E803 ◽  
Author(s):  
Tiziana Servidei ◽  
Daniela Meco ◽  
Nadia Trivieri ◽  
Valentina Patriarca ◽  
Valerio Gaetano Vellone ◽  
...  

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