scholarly journals Zooarchaeology and Ancient DNA, part 1: a brief review of the methods and applications

Author(s):  
Aurélie Manin ◽  
◽  
Ophélie Lebrasseur ◽  

Ancient DNA (aDNA) analyses from archaeological animal remains is well suited to many of the objectives investigated through zooarchaeological analyses. This paper aims at presenting the field of palaeogenomics, particularly in its application to zooarchaeological questions. While it does not seek for exhaustivity, it focuses on practical issues, hoping to promote trans-disciplinary dialogues. The authors begin with a presentation of the nature of aDNA and the main taphonomic pathways leading to its differential preservation. Then the authors offer a brief historical review of the aDNA techniques, from the early discoveries of 1984–1985 to the advent of next generation sequencing and high throughput approaches at the beginning of the 21st century. The aurhors describe the main challenges of aDNA research in recent years as it needs to adapt to a new range of methods and how the study of animal remains has been impacted. Finally, the authors highlight the main outcomes of the application of aDNA to describe animals and their populations and future lines of research that could be explored.

2019 ◽  
Vol 25 (31) ◽  
pp. 3350-3357 ◽  
Author(s):  
Pooja Tripathi ◽  
Jyotsna Singh ◽  
Jonathan A. Lal ◽  
Vijay Tripathi

Background: With the outbreak of high throughput next-generation sequencing (NGS), the biological research of drug discovery has been directed towards the oncology and infectious disease therapeutic areas, with extensive use in biopharmaceutical development and vaccine production. Method: In this review, an effort was made to address the basic background of NGS technologies, potential applications of NGS in drug designing. Our purpose is also to provide a brief introduction of various Nextgeneration sequencing techniques. Discussions: The high-throughput methods execute Large-scale Unbiased Sequencing (LUS) which comprises of Massively Parallel Sequencing (MPS) or NGS technologies. The Next geneinvolved necessarily executes Largescale Unbiased Sequencing (LUS) which comprises of MPS or NGS technologies. These are related terms that describe a DNA sequencing technology which has revolutionized genomic research. Using NGS, an entire human genome can be sequenced within a single day. Conclusion: Analysis of NGS data unravels important clues in the quest for the treatment of various lifethreatening diseases and other related scientific problems related to human welfare.


protocols.io ◽  
2021 ◽  
Author(s):  
James A ◽  
Franziska Aron ◽  
Gunnar U ◽  
Irina Velsko ◽  
Eirini Skourtanioti ◽  
...  

2012 ◽  
Vol 37 (5) ◽  
pp. 811-820 ◽  
Author(s):  
Rajeev K Varshney ◽  
Himabindu Kudapa ◽  
Manish Roorkiwal ◽  
Mahendar Thudi ◽  
Manish K Pandey ◽  
...  

protocols.io ◽  
2020 ◽  
Author(s):  
James A ◽  
Franziska Aron ◽  
Gunnar U ◽  
Irina Velsko ◽  
Eirini Skourtanioti ◽  
...  

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Wells W. Wu ◽  
Je-Nie Phue ◽  
Chun-Ting Lee ◽  
Changyi Lin ◽  
Lai Xu ◽  
...  

2019 ◽  
Vol 220 (10) ◽  
pp. 1609-1619 ◽  
Author(s):  
Sarah Wagner ◽  
David Roberson ◽  
Joseph Boland ◽  
Aimée R Kreimer ◽  
Meredith Yeager ◽  
...  

AbstractBackgroundHuman papillomaviruses (HPV) cause over 500 000 cervical cancers each year, most of which occur in low-resource settings. Human papillomavirus genotyping is important to study natural history and vaccine efficacy. We evaluated TypeSeq, a novel, next-generation, sequencing-based assay that detects 51 HPV genotypes, in 2 large international epidemiologic studies.MethodsTypeSeq was evaluated in 2804 cervical specimens from the Study to Understand Cervical Cancer Endpoints and Early Determinants (SUCCEED) and in 2357 specimens from the Costa Rica Vaccine Trial (CVT). Positive agreement and risks of precancer for individual genotypes were calculated for TypeSeq in comparison to Linear Array (SUCCEED). In CVT, positive agreement and vaccine efficacy were calculated for TypeSeq and SPF10-LiPA.ResultsWe observed high overall and positive agreement for most genotypes between TypeSeq and Linear Array in SUCCEED and SPF10-LiPA in CVT. There was no significant difference in risk of precancer between TypeSeq and Linear Array in SUCCEED or in estimates of vaccine efficacy between TypeSeq and SPF10-LiPA in CVT.ConclusionsThe agreement of TypeSeq with Linear Array and SPF10-LiPA, 2 well established standards for HPV genotyping, demonstrates its high accuracy. TypeSeq provides high-throughput, affordable HPV genotyping for world-wide studies of cervical precancer risk and of HPV vaccine efficacy.


PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151775 ◽  
Author(s):  
Avi Z. Rosenberg ◽  
Michael D. Armani ◽  
Patricia A. Fetsch ◽  
Liqiang Xi ◽  
Tina Thu Pham ◽  
...  

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