scholarly journals Studies of phosphorylation in rat mast cells. First report. Demonstration of diphosphoinositide (DPI) in rat mast cell granule membranes.

Ensho ◽  
1985 ◽  
Vol 5 (1) ◽  
pp. 31-36
Author(s):  
Motohiro Kurosawa
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Granule ◽  
First Report ◽  
Mast Cell Granule ◽  
Rat Mast Cells

scholarly journals MATURATION OF RAT MAST CELLS

1966 ◽  
Vol 31 (3) ◽  
pp. 563-575 ◽  
Author(s):  
J. W. Combs
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Granular Material ◽  
Electron Microscope Study ◽  
Cell Granule ◽  
Cell Maturation ◽  
Granule Formation ◽  
Dense Granules ◽  
Mast Cell Granule ◽  
Rat Mast Cells

Electron microscope study of rat mast cell maturation corroborates certain interpretations of features of mast cell differentiation based on light microscope studies. In addition, the ultrastructural variation observed in the granules of differentiating mast cells suggests that granule formation begins with the elaboration of dense granules about 70 mµ in diameter inside Golgi vacuoles. These progranules appear to aggregate inside a membrane and fuse to form dense cords 70 to 100 mµ in diameter. These dense cords are embedded in a finely granular material possibly added to the developing granule by direct continuity between perigranular membranes and cisternae of rough endoplasmic reticulum. The dense cords and finely granular material then appear to be replaced by a mass of strands about 30 mµ in diameter, thought to be a reorganization product of the two formerly separate components. A process interpreted as compaction of the strands completes the formation of the dense, homogeneous granules observed in mature rat mast cells. The similarity between mast cell granule formation and the elaboration of other granules is considered, with special reference to rabbit polymorphonuclear leukocyte azurophil granules. The relationships between the ultrastructural, histochemical, and radioautographic characteristics of mast cell granule formation are considered, and the significance of the perigranular membrane is discussed.

scholarly journals Preparative purification of the rat mast cell chymase. Characterization and Interaction with granule components

1977 ◽  
Vol 146 (5) ◽  
pp. 1405-1419 ◽  
Author(s):  
R Yurt ◽  
KF Austen
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cell Granule ◽  
Cationic Protein ◽  
Molar Ratios ◽  
Single Polypeptide Chain ◽  
Mast Cell Granule ◽  
Mast Cell Chymase

The rat mast cell granule chymotrypsinlike enzyme was purified to homogeneity from 1 M NaCl solubilized membrane and granule-rich fractions of concentrated rat peritoneal mast cells by a preparative technique utilizing chromatography on Dowex 1, filtration on Sephadex G-75, and affinity chromatography with D-tryptophan methyl ester. Acid disk gel electrophoresis of the purified chymase disclosed a single stained band with activity being eluted from a replicate sliced gel in the same region. SDS-polyacrylamide gel electrophoresis of purified protein gave a single stained band that did not change in position with reduction and alkylation. Mast cell chymase is thus a cationic protein of 25,000 mol wt composed of a single polypeptide chain. The apparent K(m) of the chymase for BTEE was 1.5 x 10(-3) M and the V(max) was 67.8 μmol/min per mg. The enzyme was inhibited by TPCK and not by TLCK. The chymase complexed with native macromolecular rat mast cell heparin in molar ratios of 12:1 and 16:1, and complete heparin uptake occurred at a 40:1 ratio of chymase to heparin. Chymase activity was partially masked by combination with heparin in the isolated granule or experimental chymase-heparin complex, and soluble purified chymase was inhibited by concentrations of 5-HT comparable to those present in mast cells. It is therefore possible that the active site of chymase in the mast cell granule is largely masked by the combined effects of macromolecular heparin and 5-HT.

Cytochemical analysis of mast cell granules after poly-dl-lysine action

1978 ◽  
Vol 36 (2) ◽  
pp. 278-279
Author(s):  
R. Courtoy ◽  
L.J. Simar ◽  
J. Christophe
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Compounds ◽  
Cellular Membrane ◽  
Thin Sections ◽  
Organic Bases ◽  
Ferric Thiocyanate ◽  
Cytochemical Analysis ◽  
Mast Cell Granule ◽  
Amine Liberation

Several chemical compounds induce amine liberation from mast cells but do not necessarily provoque the granule expulsion. For example, poly-dl-lysine induces modifications of the cellular membrane permeability which promotes ion exchange at the level of mast cell granules. Few of them are expulsed but the majority remains in the cytoplasm and appears less dense to the electrons. A cytochemical analysis has been performed to determine the composition of these granules after the polylysine action.We have previously reported that it was possible to demonstrate polyanions on epon thin sections using a cetylpyridinium ferric thiocyanate method. Organic bases are selectively stained with cobalt thiocyanate and the sulfhydryle groups are characterized with a silver methenamine reaction. These techniques permit to reveal the mast cell granule constituents, i.e. heparin, biogenic amines and basic proteins.

Expression, Function and Regulation of Mast Cell Granule Chymases During Mucosal Allergic Responses

2000 ◽  
pp. 257-273 ◽  
Author(s):  
Pamela A. Knight ◽  
Steven H. Wright ◽  
Elisabeth M. Thornton ◽  
Jeremy Brown ◽  
Hugh R.P. Miller
Keyword(s):  
Mast Cell ◽  
Cell Granule ◽  
Mast Cell Granule
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