scholarly journals ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT

2019 ◽  
Vol 4 (5) ◽  
pp. 215-225
Author(s):  
Arsyik Ibrahim ◽  
M. Arifuddin ◽  
Wisnu Cahyo P ◽  
Wahyu Widayat ◽  
Mahfuzun Bone
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Secondary Metabolite ◽  
Fungal Isolate ◽  
Chromatography Method ◽  
Fungal Isolates ◽  
Reaction Test ◽  
Layer Chromatography ◽  
Spray Reagents

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

scholarly journals Profil Metabolit Skunder Daun Tin (Ficus carica) melalui Analisis Histokimia dan Deteksi Flavonoid dengan Metode Kromatografi Lapis Tipis (KLT)

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Khoiria Anisa ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
Ficus Carica ◽  
Histochemical Analysis ◽  
Chromatography Method ◽  
Histochemical Test ◽  
Layer Chromatography ◽  
Descriptive Method ◽  
Test Result

Tin (Ficus carica) is one of the plants that was mentioned in the Al-Qur’an (at-Tiin 1-3). The plant has the potential of its leaves that can be used as a medicine. In this research were done two tests that are histochemical test and phytochemical test. The method used is descriptive method and the data is processed by descriptive qualitative. Histochemical analysis aims to determine the profile of secondary metabolite compounds in Tin leaf (Ficus carica); among the metabolites were tested the alkaloid, terpenoid, phenol, flavonoid and lipophilic. The second test is the phytochemical test to the examine flavonoid aglycon present, among the tested it  is anthocyanin, flavones, flavonol and, biflavonil by thin layer chromatography method. The result showed that the histochemical analysis of Tin leaf (Ficus carica) contain secondary alkaloid, phenol, flavonoid and lipophilic. The phytochemical test result is flavonoid aglycon that show existence is anthocyanin, flavones and biflavonil.Keywords: Tin (Ficus carica), Histochemical profile, Phytochemical test, Flavonoid, TLC.ABSTRAKTin (Ficus carica) adalah salah satu tanaman yang disebutkan di dalam kitab suci Al-Qur’an (Surat At-Tiin 1-3) yang memiliki potensi pada daunnya dapat digunakan sebagai obat. Telah dilakukan penelitian analisis histokimia dan uji fitokimia daun Tin (Ficus carica). Metode yang digunakan dalam penelitian ini ialah metode deskriptif. Data diolah secara deskriptif kualitatif. Analisis histokimia bertujuan untuk mengetahui profil senyawa metabolit sekunder dalam daun Tin (Ficus carica); di antara uji metabolit dilakukan uji alkaloid, terpenoid, fenol, flavonoid dan lipofilik. Uji fitokimia bertujuan untuk memeriksa aglikon flavonoid yang ada, di antara aglikon yang diuji yaitu antosianin, flavon, flavonol dan biflavonil dengan menggunakan metode kromatografi lapis tipis (KLT). Hasil penelitian menunjukkan bahwa analisis histokimia daun Tin (Ficus carica) terdeteksi mengandung senyawa metabolit sekunder alkaloid, fenol, flavonoid dan lipofilik. Hasil uji fitokimia menunjukkan adanya senyawa antosianin, flavon dan biflavonil.Kata kunci: Tin (Ficus carica), Profil histokimia, Uji fitokomia, Flavonoid, KLT.

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