scholarly journals Isolation and Characterization of Secondary Metabolites of Asplenium indicum by Using Thin Layer Chromatography

2021 ◽  
Vol 65 (6) ◽  
pp. 137-141
Author(s):  
Damayanti Jadhav ◽  
Manda Ghatage
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Isolation And Characterization ◽  
Layer Chromatography

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

scholarly journals Senyawa Saponin Hasil Isolasi dari Daun Buni (Antidesma bunius (L.) Spreng.)

2018 ◽  
Vol 1 (1) ◽  
pp. 264-270
Author(s):  
Hady Wiraputra ◽  
Marline Nainggolan ◽  
Panal Sitorus
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Preparative Thin Layer Chromatography ◽  
Ash Content ◽  
Hydroxyl Group ◽  
Chloroform Fraction ◽  
Aliphatic Group ◽  
Layer Chromatography

Tanaman buni (Antidesma bunius (L.) Spreng.) secara tradisional telah digunakan untuk hipertensi, takikardia, anemia, sifilis, antikanker, antioksidan, sumber pewarna alami dan antidiabetes. Saponin merupakan senyawa fitokimia yang mempunyai kemampuan membentuk busa dan mengandung aglikon polisiklik yang berikatan dengan satu atau lebih gula. Penelitian ini bertujuan untuk melakukan karakterisasi senyawa saponin hasil isolasi dari daun buni dengan spektrofotometer ultraviolet dan inframerah. Simplisia daun buni dilakukan karakterisasi kemudian diekstraksi dengan cara maserasi bertingkat menggunakan pelarut n-heksana dan etanol 80%. Selanjutnya ekstrak etanol dihidrolisis dengan HCl 2N kemudian difraksi dengan pelarut kloroform. Isolasi dilakukan terhadap fraksi kloroform dengan cara kromatografi lapis tipis preparatif menggunakan fase diam silika gel GF254 dan fase gerak yang sesuai. Isolat yang diperoleh diuji kemurnian dengan KLT 2 arah dan dikarakterisasi menggunakan spektrofotometer ultraviolet dan inframerah. Hasil pemeriksaan karakterisasi simplisia diperoleh kadar air 7,32%, kadar sari larut dalam etanol 52,70%, kadar sari larut dalam air 23,25%, kadar abu total 6,86% dan kadar abu tidak larut dalam asam 0,94%. Pemisahan fraksi kloroform dengan KLT menggunakan fase gerak n-heksana-etilasetat perbandingan 5:5 diperoleh noda 13 dan hasil KLT preparatif diperoleh 2 isolat murni yaitu isolat 1 (ungu merah) dengan Rf 0,92 dan isolat 2 (biru) dengan Rf 0,78. Hasil karakterisasi isolat 1 diperoleh panjang gelombang maksimum pada 208 nm dan dijumpai adanya gugus hidroksil, gugus -CH alifatis, ikatan C=C, gugus –CH2, gugus –CH3, dan gugus C-O. Hasil karakterisasi isolat 2 diperoleh panjang gelombang maksimum pada 204 nm dan adanya gugus hidroksil, gugus -CH alifatis, gugus –CH2, gugus –CH3, dan gugus C-O. Buni (Antidesmabunius (L.) Spreng.) has been traditionally used for the treatment of hypertension, tachycardia, anemia, syphilis, and used asanti-cancer, anti-oxidant, natural dye, and anti-diabetic. Saponin is a phytochemical compound which has capability in forming foam and contains polycyclic aglycone that binds with one or more glucose. This research aimed to conduct the characterization of saponin compound from buni leaves with ultraviolet spectrophotometer and infrared. Buni leaves simplicia was characterizedand extracted using sequential maceration method with n-hexane and 80% ethanol. The ethanol extract was hydrolyzed with HCl 2N and fractionized using chloroform solvent. Isolation of chloroform fraction was done using preparative thin-layer chromatography using silent phase of silica gel GF 254 and suitable mobile phase. Isolates obtained was taken into purity test with two dimensions thin-layer chromatography and characterized using ultraviolet spectrophotometer and infrared. The characterized simplicia resulted with 7.32% of water content, 52.70% of dissolved content in ethanol, 23.25% of dissolvedcontent in water, 6.86% of total ash content, and 0.94% of undissolved ash content in acid. Fractinationof chloroform fraction with thin-layer chromatography using mobile phase ofn-hexane-ethyl acetate with 5:5 ration resulted with 13 spotsand the result of the preparative thin-layer chromatography resulted 2 pure isolates which are isolate 1 (purple-red) with Rf 0.92 and isolate 2 (blue) with Rf 0.78. The characterization of isolate 1 resulted that the maximum wave lengthwas 208 nm with hydroxyl group, –CH aliphatic group, C=C bond, –CH2 group, –CH3 group, and C–O group. The characterization of isolate 2 resulted that the maximum wave lengthwas204 nm with hydroxyl group, –CH aliphatic group, –CH2 group, –CH3 group, and C–O group.

scholarly journals ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT

2019 ◽  
Vol 4 (5) ◽  
pp. 215-225
Author(s):  
Arsyik Ibrahim ◽  
M. Arifuddin ◽  
Wisnu Cahyo P ◽  
Wahyu Widayat ◽  
Mahfuzun Bone
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Secondary Metabolite ◽  
Fungal Isolate ◽  
Chromatography Method ◽  
Fungal Isolates ◽  
Reaction Test ◽  
Layer Chromatography ◽  
Spray Reagents

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

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