scholarly journals IMMUNE CYTOPENIAS IN PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA (PECULIARITIES, PROGNOSTIC MARKERS)

2021 ◽  
Vol 65 (2) ◽  
Author(s):  
Olha Vygovska ◽  
◽  
Nataliia Knysh ◽  
Maryana Simonova ◽  
Tetiana Horodyska ◽  
...  
Keyword(s):  
Overall Survival ◽  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Prognostic Markers ◽  
Cytogenetic Study ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Coombs Test ◽  
Evans Syndrome ◽  
Blood Lymphocytes

Background: Immune cytopenia (IC) is one of the major complications in chronic lymphocytic leukemia (CLL). The paper describes the peculiarities of different immune cytopenia in CLL patients and the importance of individual prognostic markers in the course of the disease. Methods: We observed 62 patients with CLL complicated by immune cytopenia. Among these patients 30 had autoimmune hemolytic anemia (AIHA), 18 experienced immune thrombocytopenia (ITP), 10 had Fisher-Evans syndrome (FES), 3 were diagnosed with partial red cell aplasia (PRCA), and immune neutropenia (IN) was revealed in 1 patient. In addition to general examination and laboratory studies, the following examinations were performed: immunophenotyping of peripheral blood lymphocytes, flow cytometry (CD5; CD19; CD20; CD23; CD38; ZAP70), Coombs test, a molecular cytogenetic study of peripheral blood lymphocytes using the FISH method with TP53 and ATM probes, the level of ß2-microglobulin. Results: It was established that the overall survival of CLL patients with IC depends on the form of the latter. The median overall survival in patients with Fisher-Evans syndrome was the shortest (75 months), slightly better survival was observed in patients with AIHA (median 80 months), the best survival was found in patients with ITP (median not reached). Among unfavorable markers of CLL with IC, there is the presence of del 11q22.3. Unfavorable prognostic markers were also the following: a positive Coombs test, high levels of ZAP 70 expression, and high levels of ß2-microglobulin

scholarly journals Visualization of tubulin in lymphocytes. I. Comparison of normal and chronic lymphocytic leukemia (CLL) lymphocytes

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1031-1037 ◽  
Author(s):  
G Dighiero ◽  
E Karsenti ◽  
JY Follezou ◽  
M Bornens
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Normal Subjects ◽  
Peripheral Blood Lymphocytes ◽  
Lower Number ◽  
Lymphocytic Leukemia ◽  
Blood Lymphocytes ◽  
Cell Structures ◽  
Immunoperoxidase Assay

Abstract Cell structures containing tubulin were studied in peripheral blood lymphocytes from 8 normal donors and 11 patients with CLL using specific antitubulin antibodies revealed by immunoperoxidase assay. The centriole and microtubules were clearly visible in both groups. A “nucleus-associated tubulin-containing structure” was revealed by antitubulin antibodies and was found in virtually all lymphocytes of normal subjects but in a considerably lower number of CLL lymphocytes. The nature of this structure and its relationship to other cell structures are discussed.

scholarly journals Changes in Glycanic Determinants of Lymphocytes Membranes in Peripheral Blood in Patients with B-Cell Chronic Lymphocytic Leukemia under Antitumor Therapy

2021 ◽  
Vol 6 (6) ◽  
pp. 141-147
Author(s):  
G. S. Maslak ◽  
◽  
G. P. Chernenko ◽  
V. M. Baibakov ◽  
A. D. Viselko ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
B Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Cytostatic Therapy ◽  
Tn Antigen ◽  
Antitumor Therapy ◽  
Blood Lymphocytes ◽  
Cell Chronic Lymphocytic Leukemia

The purpose of the study was to study the nature of changes in the exposure of surface glycans of peripheral blood lymphocytes in patients with B-cell chronic lymphocytic leukemia under conditions of antitumor therapy. Materials and methods. We studied the features of exposure of surface glycotopes of peripheral blood lymphocytes in patients with B-cell chronic lymphocytic leukemia under conditions of antitumor therapy using a set of seven lectins labeled with FITC and monoclonal antibodies to Tn-antigen- FITC for the detection of Tn antigen and CD43 exposure on blood lymphocytes. Cytostatic therapy included cyclophosphamide, vincristine (oncovin), prednisolone. Data were recorded on a Beckman Coulter EPICS flow cytometer. The results were processed using FCS3 Express. Results and discussion. The number of lymphocytes of healthy donors with a positive reaction to ConA, PHA-L, SNA, MAA-II and α1-acid glycoprotein amounted to 16.0±3.0%, 23.0±2.3%, 15.0±1.5%, 25.0±1.8% and 15.0±1.3%, respectively. The number of LABA-, UEA I-positive lymphocytes was 0.90±0.03% and 2.9±0.2%, respectively, and there was no binding to antibodies to Tn- and CD43-antigens. In the blood of patients with chronic lymphocytic leukemia, the level of ConA-, SNA- and MAA-II-positive lymphocytes increased relative to control by 2.2, 3.7 and 2.6 times, respectively. The number of LABA- and UEA I-positive lymphocytes in patients with chronic lymphocytic leukemia increased by 11 (p <0.01) and 23 (p <0.001) times and amounted to 10.5±0.5% and 67.5±5.5% respectively. The number of lymphocytes with CD43 antigen on their surface increased by 72 times, and the Tn antigen increased by 80 times. Cytostatic therapy reduced the level of LABA- and UEA I-positive lymphocytes by almost half, and MAA II-positive cells and lymphocytes interacting with antibodies to CD43 and Tn antigen by a third. The level of PHA-L-positive lymphocytes in the blood of chronic lymphocytic leukemia patients after undergoing alkylating therapy increased by 18.0±2.0% and almost did not differ from those obtained in the control group. Conclusion. 1. In chronic lymphocytic leukemia patients, the structure of glycoconjugates in peripheral blood lymphocytes changes, manifested in increased exposure of L-fucose, α-mannose and N-acetylneuraminic acid, which is confirmed by a significant increase in relation to the control of the number of ConA-, SNA-, MAA-II-, LABA I-positive cells. 2. Patients with chronic lymphocytic leukemia showed a significant increase in the number of lymphocytes, in which the markers of carcinogenesis CD43 and Tn antigens were found. 3. Cytostatic therapy significantly reduced the level of LABA-, UEA I- and MAA II-positive cells, as well as partially Tn- and CD43-antigen-positive lymphocytes, which indicates its positive effect on the treatment of chronic lymphocytic leukemia

scholarly journals Visualization of tubulin in lymphocytes. I. Comparison of normal and chronic lymphocytic leukemia (CLL) lymphocytes

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1031-1037
Author(s):  
G Dighiero ◽  
E Karsenti ◽  
JY Follezou ◽  
M Bornens
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Normal Subjects ◽  
Peripheral Blood Lymphocytes ◽  
Lower Number ◽  
Lymphocytic Leukemia ◽  
Blood Lymphocytes ◽  
Cell Structures ◽  
Immunoperoxidase Assay

Cell structures containing tubulin were studied in peripheral blood lymphocytes from 8 normal donors and 11 patients with CLL using specific antitubulin antibodies revealed by immunoperoxidase assay. The centriole and microtubules were clearly visible in both groups. A “nucleus-associated tubulin-containing structure” was revealed by antitubulin antibodies and was found in virtually all lymphocytes of normal subjects but in a considerably lower number of CLL lymphocytes. The nature of this structure and its relationship to other cell structures are discussed.

scholarly journals HLA-DR-associated invariant chain is highly expressed in chronic lymphocytic leukemia

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 372-377
Author(s):  
F Narni ◽  
J Kudo ◽  
W Mars ◽  
B Calabretta ◽  
DL Florine ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Leukemic Cell ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Invariant Chain ◽  
Blood Lymphocytes ◽  
Normal Tissues ◽  
Hla Dr ◽  
Leukocyte Populations

Total RNA extracted from peripheral blood lymphocytes of a patient with B-cell chronic lymphocytic leukemia (CLL) and the poly (A+) RNA was purified. A cDNA library was constructed and approximately 4,000 clones were screened in order to identify genes preferentially expressed in CLL. A relatively low repetition frequency characterizes the majority of the abundant mRNA species present in CLL lymphocytes. One clone, corresponding to the mRNA encoding the HLA-DR-associated invariant chain, was selected and its expression was examined in different leukemic cell populations and in normal tissues. DNA-RNA hybridization studies showed that the invariant chain mRNA (In-mRNA) is detectable in RNA preparations from human blood cells and their precursors, whereas no In-mRNA is found in several other tissues examined. Among various normal and leukemic leukocyte populations, the highest levels of In- mRNA are found in CLL. Therefore, a role of In-chain mRNA as a marker of CLL is proposed. Our data support a relationship between high levels of invariant chain mRNA and the out of cycle condition of CLL peripheral blood lymphocytes.

De novo purine nucleotide synthesis in total peripheral blood lymphocytes from patients with B-chronic lymphocytic leukemia (B-CLL)

1994 ◽  
Vol 22 (2) ◽  
pp. 241S-241S ◽  
Author(s):  
DISPENSA EGIDIO ◽  
FILIPPO CARLUCCI ◽  
ELENA CONSOLMAGNO ◽  
PIERO GALIENI ◽  
ROBERTO LEONCINI ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
De Novo ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Purine Nucleotide ◽  
Nucleotide Synthesis ◽  
Blood Lymphocytes

Expression of the 5'-nucleotidase gene in the peripheral blood lymphocytes from B-chronic lymphocytic leukemia. Evaluation of mRNA

1996 ◽  
Vol 24 (1) ◽  
pp. 49S-49S
Author(s):  
Maria Pizzichini ◽  
Maria D'apolito ◽  
Francesca Rosi ◽  
Daniela Vannoni ◽  
Roberto Leoncini ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Blood Lymphocytes
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