scholarly journals Studi Awal Pengaruh Radiasi Terhadap Pekerja Radiasi Menggunakan Metode Comet Assay

2019 ◽  
Vol 11 (1) ◽  
pp. 9-15
Author(s):  
Amel Oktavia S ◽  
Dian Milvita ◽  
Darlina Darlina
Keyword(s):  
Comet Assay ◽  
Tail Length ◽  
Dna Comet Assay ◽  
Film Badge

Telah dilakukan studi awal pengaruh radiasi terhadap pekerja radiasi menggunakan metode comet assay. Pengambilan data dosis radiasi dilakukan pada hasil pembacaan laju dosis radiasi terhadap pekerja radiasi menggunakan film badge di Instalasi Radiologi RS Dr. Reksodiwiryo Padang, sedangkan pemeriksaan DNA sel limfosit dilakukan di Laboratorium Biomolekuler PTKMR BATAN. Pemeriksaan DNA sel limfosit dilakukan pada 50 sel sampel darah dari tiga orang pekerja radiasi dan tiga orang normal sebagai sampel kontrol, menggunakan mikroskop fluorescent dan CASP LAP comet assay software. Hasil pembacaan dosis radiasi menunjukkan bahwa laju dosis radiasi yang diterima pekerja radiasi yaitu 1,2 mSv/tahun. Hasil ini masih berada di bawah NBD berdasarkan Perka BAPETEN No 4 Tahun 2013. Pemeriksaan DNA sel limfosit menunjukkan nilai rata-rata tail length (TL) sampel pekerja radiasi lebih tinggi dibandingkan dengan sampel kontrol. Berdasarkan nilai TL tersebut belum bisa diketahui tingkat keparahan suatu kerusakan DNA.Kata kunci : laju dosis radiasi, NBD, kerusakan DNA , comet assay, limfosit, tail length

Impact of gamma irradiation on tissues of the mud crab, Scylla serrata (Forskål, 1775) (Decapoda, Portunidae) — electron microscopic study and DNA comet assay

Crustaceana ◽  
2019 ◽  
Vol 92 (1) ◽  
pp. 33-56
Author(s):  
Arshad Keethadath ◽  
Sudha Kappalli ◽  
N. Gayathri ◽  
Derin M. Thomas ◽  
Gopinathan Anilkumar
Keyword(s):  
Comet Assay ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Tail Length ◽  
Significant Degree ◽  
Scylla Serrata ◽  
Dependent Manner ◽  
Electron Microscopic ◽  
Dna Comet Assay ◽  
Sarcotubular System

Abstract Radiopreservation using gamma radiation is widely in use as a safe method for extending the shelf life of shellfish. This study explored the consequences of different doses of gamma radiation (0.5 kGy, 1.0 kGy and 2.0 kGy) on various tissues of Scylla serrata at cellular and nuclear level, with the aid of electron microscopy and DNA comet assay. The highly radio exposed (2.0 kGy) pyloric muscles showed a reduction in sarcomere length, disordered organization with expanded gap between adjacent myofibrils, ruptured sarcotubular system, mitochondrial swelling with crushed cristae, significant increase in nucleus size coupled with less dense nucleoplasm, etc. Comet assay on tissues such as muscle, hepatopancreas and testis irradiated with 2.0 kGy radiation also revealed a significant degree of nuclear damage by gamma irradiation in a dose-dependent manner. The tail length of the comet showed a tissue-specific tolerance level. The present study clarified the precise dose of irradiation as 1.0 kGy and the results can be relevant for commercial purposes to qualitatively categorize the irradiated crabs.

Identification of irradiated refrigerated poultry with the DNA comet assay

2004 ◽  
Vol 71 (1-2) ◽  
pp. 189-191 ◽  
Author(s):  
A.L.C.H Villavicencio ◽  
M.M Araújo ◽  
N.S Marin-Huachaca ◽  
J Mancini-Filho ◽  
H Delincée
Keyword(s):  
Comet Assay ◽  
Dna Comet Assay

Use of the DNA Comet Assay to detect beef meat treated by ionizing radiation

Meat Science ◽  
2005 ◽  
Vol 71 (3) ◽  
pp. 446-450 ◽  
Author(s):  
N. Marín-Huachaca ◽  
H. Delincée ◽  
J. Mancini-Filho ◽  
A.L.C.H. Villavicencio
Keyword(s):  
Ionizing Radiation ◽  
Comet Assay ◽  
Dna Comet Assay ◽  
Beef Meat

scholarly journals Evaluation of irradiation in foods using DNA Comet assay

2010 ◽  
Vol 48 (1) ◽  
pp. 106-109 ◽  
Author(s):  
Affaf Khawar ◽  
Ijaz Ahmad Bhatti ◽  
Q. M. Khan ◽  
A. I. Khan ◽  
M. R. Asi ◽  
...  
Keyword(s):  
Comet Assay ◽  
Dna Comet Assay

Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis

2009 ◽  
Vol 78 (9) ◽  
pp. 776-781 ◽  
Author(s):  
Yakup Erel ◽  
Nizamettin Yazici ◽  
Sumer Özvatan ◽  
Demet Ercin ◽  
Nurcan Cetinkaya
Keyword(s):  
Image Analysis ◽  
Comet Assay ◽  
Dna Comet Assay

scholarly journals Comet Assay results of pilots exposed to pesticides

2018 ◽  
Vol 28 (5) ◽  
pp. 9-18
Author(s):  
Carmen Martínez-Valenzuela ◽  
Stefan Waliszewski ◽  
María Elena Calderón-Segura ◽  
Mario Caba ◽  
Enrique Meza ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Tail Length ◽  
Study Groups ◽  
Cultivated Plants ◽  
P Value ◽  
Exposed Workers ◽  
Occupationally Exposed ◽  
Post Hoc ◽  
Organic Pesticides

Pesticides constitute a heterogeneous category of chemicals designed for the control of pests affecting cultivated plants. Frequently, they are classified according to their chemical structure, organic and non-organic pesticides. Biomonitoring studies using somatic cells have been conducted to evaluate the possible genotoxic risk of occupationally exposed workers to pesticides. The aim of this study was to assess the genotoxic effects of pesticides in pilots occupationally exposed to these chemicals during aerial application in agricultural fields. The study groups comprised 30 pilots who applied aerial pesticides and 30 controls. The alkaline Comet Assay was performed on freshly collected peripheral whole blood lymphocytes. The nonparametric Mann-Whitney test was applied to compare the equality of two population medians. Additionally, a comparison of two groups according to age and years of work as quantitative variables and a one-way analysis of variance (Anova) with Tukey’s post hoc test were applied. To corroborate differences between groups, a regression analysis was performed to calculate the degree of correlation, expressing their magnitude by R2. Statistical significances were set at a p value of <0.05. The median of comet frequency, tail length (159.6 ± 16.8) and tail moment (16.75 ± 3.13) reveals statistically significant differences (p < 0.001) between exposed pilots and controls. The pilot group divided according to age reveals Deoxyribonucleic acid (DNA) damage which increases significantly when age of participant increases. Neither smoking nor alcohol consumption could be statistically linked to DNA damage. 

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