beef meat
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2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Kiandokht Babolhavaeji ◽  
Leili Shokoohizadeh ◽  
Morteza Yavari ◽  
Abbas Moradi ◽  
Mohammad Yousef Alikhani

Background. The aims of the current study are the identification of O157 and non-O157 Shiga Toxin-Producing Escherichia coli (STEC) serogroups isolated from fresh raw beef meat samples in an industrial slaughterhouse, determination of antimicrobial resistance patterns, and genetic linkage of STEC isolates. Materials and Methods. A total of 110 beef samples were collected from the depth of the rump of cattle slaughtered at Hamadan industrial slaughterhouse. After detection of E. coli isolates, STEC strains were identified according to PCR for stx1, stx2, eaeA, and hlyA virulence genes, and STEC serogroups (O157 and non-O157) were identified by PCR. The genetic linkage of STEC isolates was analyzed by the ERIC- (Enterobacterial Repetitive Intergenic Consensus-) PCR method. The antimicrobial susceptibility of STEC isolates was detected by the disk diffusion method according to CLSI guidelines. Results. Among 110 collected beef samples, 77 (70%) were positive for E. coli. The prevalence of STEC in E. coli isolates was 8 (10.4%). The overall prevalence of O157 and non-O157 STEC isolates was 12.5% (one isolate) and 87.5% (7 isolates), respectively. The hemolysin gene was detected in 25% (2 isolates) of STEC strains. Evaluation of antibiotic resistance indicated that 100% of STEC isolates were resistant to ampicillin, ampicillin-sulbactam, amoxicillin-clavulanic acid, and cefazolin. Resistance to tetracycline and ciprofloxacin was detected in 62.5% and 12.5% of isolates, respectively. The analysis of the ERIC-PCR results showed five different ERIC types among the STEC isolates. Conclusion. The isolation of different clones STECs from beef and the presence of antibiotic-resistant isolates indicate that more attention should be paid to the hygiene of slaughterhouses.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3517
Author(s):  
Chenchen Xu ◽  
Shou Zhang ◽  
Baozhong Sun ◽  
Peng Xie ◽  
Xiaochang Liu ◽  
...  

The purpose of this study was to evaluate the effects of dietary supplementation with microalgae (Schizochytrium sp.) containing docosahexaenoic acid (DHA) on the antioxidant enzyme activity, physicochemical quality, fatty acid composition and volatile compounds of beef meat. Eighteen male Qaidamford cattle were randomly allocated into three treatments (n = 6): no micro-algae supplementation (Control group, C), 100 g microalgae supplementation per bull per day (FD1), and 200 g microalgae supplementation per bull per day (FD2), and fed for 49 days before slaughter. The results showed that, compared with the C group, the addition of DHA-rich microalgae to the diet could significantly increase the total antioxidant capacity (T-AOC) in meat. In the FD2 group, it was found that the concentration of glutathione peroxidase (GSH-Px) was significantly higher than that of the control group (p < 0.05). DHA-rich microalgae supplementation increased polyunsaturated fatty acid (PUFA), eicosapentaenoic acid (EPA; C20:5 n-6), DHA, EPA + DHA, and n-3 PUFA and reduced n-6:n-3 fatty acid ratio. Twenty-four volatile compounds identified in beef were mainly aldehydes, alcohols and ketones from the fingerprints. The contents of short-chain fatty aldehydes, 1-octen-3-ol and 2-pentylfuran, were higher in the FD2 group than in the other two groups. The microalgae diet improved the sensory attribute score of beef. The results demonstrated that dietary supplementation of DHA-rich microalgae improved the antioxidant status, increased the deposition of DHA and enhanced the characteristic flavor of beef.


2021 ◽  
Vol 10 (16) ◽  
pp. e330101623847
Author(s):  
Matheus Campos Castro ◽  
Eloize Silva Alves ◽  
Bruno Henrique Figueiredo Saqueti ◽  
Jéssica Souza Alves ◽  
Joice Camila Martins Costa ◽  
...  

O presente estudo tem como objetivo fornecer uma compreensão sobre a relação das condições do bem-estar animal antecedentes ao abate à qualidade da carne baseada em uma revisão bibliográfica. Para isso foi realizado uma busca na literatura utilizando as seguintes palavras-chaves: Welfare, Animal Welfare, Pre-slaughter, Beef, Meat Quality. A partir do início do século XXI foi possível observar um crescente aumento de pesquisas pertencentes ao tema, isso acontece devido ao fato de que cada vez mais o bem-estar animal está relacionado ao estresse do animal, metabolismo durante o rigor mortis – responsável pela conversão do tecido muscular em carne de consumo, assim consequentemente os consumidores finais estão cada vez mais exigentes em relação a isso. No pós-abate de bovinos, podem surgir defeitos da qualidade da carne, denominadas como PSE (pálido, mole, exsudativo) e DFD (escuro, duro, seco), associados a um pH anormal da carne. O bem-estar animal está intimamente ligado desde a criação dos animais até a etapa de pré-abate da carne. A partir do estudo realizado, pode-se definir a necessidade de novas pesquisas que avaliem métodos de prevenção de estresse durante ao pré-abate.


Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2802
Author(s):  
Konan Charles Aimeric N’Gatta ◽  
Alain Kondjoyan ◽  
Raphael Favier ◽  
Jacques Rouel ◽  
Annie Vénien ◽  
...  

Tenderness is a major factor in consumer perception and acceptability of beef meat. Here we used a laboratory tumbling simulator to investigate the effectiveness of the tumbling process in reducing the toughness of raw beef cuts. Twelve Semitendinosus beef muscles from cows were tumbled according to four programs: T1 (2500 consecutive compression cycles (CC), for about 3 h), T2 (6000 CC, about 7.5 h), T3 (9500 CC, about 12 h), and T4 (13,000 CC, about 16 h). The effect of tumbling on the toughness of raw meat was assessed using compression tests (stresses measured at 20% and 80% of deformation ratios) and microscopic observations made at the periphery and centre of meat samples, and compared against non-tumbled controls. Longer tumbling times significantly reduced the stresses measured at 20% and 80% compression rates, which reflected the toughness of muscle fibres and connective tissue, respectively. At the microscopic level, longer tumbling times led to reduced extracellular spaces, increased degradation of muscle structure, and the emergence of amorphous zones. A 12-h tumbling protocol ultimately makes the best compromise between the process time demand and toughness reduction in beef Semitendinosus meat pieces.


Author(s):  
Jair Castro de Jesús ◽  
María E. Ortega-Cerrilla ◽  
José G. Herrera-Haro ◽  
Aleida S. Hernández-Cazares ◽  
Julio M. Ayala-Rodríguez

Objective: To review how transport and stunning of cattle affect animal welfare. Approach: During the transport of beef cattle to slaughter plants, several factors affect animal welfare, such as travel time, stress, and load density. Additionally, the correct stunning of cattle helps comply with the animal welfare guidelines established by different protocols such as Welfare Quality®. Study limitations/Implications: Meat quality is affected by several factors, being of utmost importance the way animals are transported to the slaughterhouse, and they are stunned. Therefore, it is critical to perform these stages properly to obtain good quality meat; besides, it is a welfare issue. Conclusions: It is critical to comply with transport and slaughter procedures that guarantee good beef meat quality and ensure animal welfare to avoid stress in cattle as possible.


2021 ◽  
pp. 2827-2832
Author(s):  
Yaser H. Tarazi ◽  
Saeb N. El-Sukhon ◽  
Zuhair Bani Ismail ◽  
Amani A. Almestarehieh

Background and Aim: Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen with worldwide distribution. Data regarding its presence, distribution, virulence, and antimicrobial susceptibility among various animal species and humans in Jordan are lacking. Therefore, the objectives of this study were to isolate and characterize EHEC from human and animal diarrhea fecal samples and ground beef samples. Materials and Methods: A total of 100 and 270 diarrhea fecal samples from humans and animals, respectively, were collected. In addition, 40 ground beef meat samples were collected from retail markets. EHEC was positively identified by detecting Shiga toxins (stx1 and stx2) genes using multiplex polymerase chain reaction (PCR). Antimicrobial susceptibility patterns were determined using the disk diffusion test. Beta-lactamase production was detected using the double disk diffusion test and the extended-spectrum beta-lactamases (ESBLs) were identified by detection of blaTEM, blaSHV, and OXA-1 genes using multiplex PCR. Pulsed-field gel electrophoresis (PFGE) was used to investigate the relatedness of EHEC isolates from different sources. Results: Out of 410 samples, 194 E. coli isolates were positively identified, of which 57 isolates (29%) were classified as EHEC. Thirty-five (61%) of EHEC isolates were serotyped as O157 (19: O157:H7 and 16: O157:NM). The stx1 gene was detected only among the sheep and goats isolates at a rate of 7.6% and 5.2%, respectively, while the stx2 gene was detected in only one ground beef meat sample. EHEC isolates showed high resistance patterns against amoxicillin, gentamycin, cephalexin, and doxycycline. Twenty-four out of 32 EHEC isolates were determined as ESBL producers, among which 14 isolates expressed the blaSHV gene and 19 isolates expressed the blaTEM while four expressed both genes. PFGE analysis revealed two clusters with high similarity (92%) originated from ground beef meat and cattle fecal samples. No similarities were found between human and animal E. coli isolates. Conclusion: Results of this study indicate widespread ESBL EHEC among humans, animals, and ground beef meat samples. These results represent an important alarm that requires the implementation of appropriate preventative measures by both human and animal health sectors to prevent the transmission of this important foodborne pathogen.


Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2415
Author(s):  
Diane de La Pomelie ◽  
Sabine Leroy ◽  
Régine Talon ◽  
Philippe Ruiz ◽  
Philippe Gatellier ◽  
...  

Escherichia coli O157:H7 is responsible for foodborne poisoning, incriminating contaminated animal food and especially beef meat. This species can survive in the digestive tract, but, up to now, very few studies have considered its survival during the gastrointestinal digestion of meat. The present study aimed to investigate the survival of the pathogenic strain E. coli O157:H7 CM454 during the gastrointestinal digestion of ground beef meat and its interactions with meal components using a semidynamic digestive model. The CM454 strain in meat survived throughout digestion despite acidic pH (pH 2) and the presence of bile salts. The addition of nitrite and ascorbate in the digestion medium led to a decrease in strain survival. During digestion, a release of free iron was observed, which was accentuated in the presence of the CM454 strain. In addition, the strain modified the Fe2+/Fe3+ ratio, in favor of Fe2+ compared to the noninoculated meat sample. In the presence of nitrite, nitroso compounds such as nitrosamines, nitrosothiols, and nitrosylheme were formed. E. coli O157:H7 CM454 had no impact on N-nitrosation but seemed to decrease S-nitrosation and nitrosylation.


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