Production of monoclonal antibodies reactive with ochratoxin A: Enzyme-linked immunosorbent assay for detection of ochratoxin A

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

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Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies

Journal of Virological Methods ◽

10.1016/0166-0934(89)90006-2 ◽

1989 ◽

Vol 24 (1-2) ◽

pp. 43-56 ◽

Cited By ~ 7

Author(s):

Giuseppe Gerna ◽

Antonella Sarasini ◽

Angela Di Matteo ◽

Maurizio Parea ◽

Maria Torseilini ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Immunosorbent Assay ◽

Rapid Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Human Rotavirus

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Simultaneous determination of glycyrrhizin and liquiritin in licorice roots and Kampo medicines by combination enzyme-linked immunosorbent assay using anti-glycyrrhizin and anti-liquiritin monoclonal antibodies

Journal of Immunoassay and Immunochemistry ◽

10.1080/15321819.2016.1260586 ◽

2016 ◽

Vol 38 (3) ◽

pp. 285-298 ◽

Cited By ~ 2

Author(s):

Shunsuke Fujii ◽

Osamu Morinaga ◽

Takuhiro Uto ◽

Shuichi Nomura ◽

Yukihiro Shoyama

Keyword(s):

Monoclonal Antibodies ◽

Simultaneous Determination ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay

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MYCOTOXINS IN BEANS OF PEANUTS GROWN IN TAJIKISTAN

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.201804011 ◽

2018 ◽

Vol 1 (4) ◽

pp. 74-77

Author(s):

Sh. I. Razokov ◽

◽

D. M. Mirzoev ◽

G. P. Kononenko ◽

A. A. Burkin ◽

...

Keyword(s):

Ochratoxin A ◽

Immunosorbent Assay ◽

Cyclopiazonic Acid ◽

Practical Significance ◽

Enzyme Linked Immunosorbent Assay ◽

Test Systems ◽

The Republic ◽

Combined Contamination

The article presents the results of an extensive mycotoxicological examination of 11 samples of peanut beans grown in two regions of the Republic of Tajikistan. The determination of 16 mycotoxins was carried out by indirect competitive enzyme-linked immunosorbent assay using commercial and certified research test systems. It has been established that for peanut beans in this area, a combined contamination by a group of sanitary-significant mycotoxins, including diacetoxyscirpenol, alternariol, ochratoxin A, PR-toxin and cyclopiazonic acid, is characteristic. The prospects of further research and the practical significance of the results are discussed.

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Development of a quantitative and sensitive enzyme-linked immunosorbent assay for ochratoxin A using antibodies from the yolk of the laying hen

Journal of Agricultural and Food Chemistry ◽

10.1021/jf00034a050 ◽

1993 ◽

Vol 41 (10) ◽

pp. 1784-1789 ◽

Cited By ~ 40

Author(s):

J. R. Clarke ◽

R. R. Marquardt ◽

A. Oosterveld ◽

A. A. Frohlich ◽

F. J. Madrid ◽

...

Keyword(s):

Ochratoxin A ◽

Immunosorbent Assay ◽

Laying Hen ◽

Enzyme Linked Immunosorbent Assay

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Mycotoxins as a risk in the grain food

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn0917079m ◽

2009 ◽

pp. 79-86 ◽

Cited By ~ 10

Author(s):

Jovana Matic ◽

Jasna Mastilovic ◽

Ivana Cabarkapa ◽

Anamarija Mandic

Keyword(s):

European Union ◽

Secondary Metabolites ◽

Ochratoxin A ◽

Immunosorbent Assay ◽

Toxic Effects ◽

Enzyme Linked Immunosorbent Assay ◽

The European Union ◽

The Mean ◽

Grain Foods

Mycotoxins are toxic secondary metabolites of fungi that contaminate a large variety of foods and have toxic effects on humans. The best protection against mycotoxins is to monitor their presence in food. This paper shows the screening results of mycotoxins present in 76 samples of different groups of grain foods. Samples of grain food were analyzed for contamination with aflatoxins, ochratoxin A, zearalenone, fumonisins and deoxynivalenol. Analysis were conducted using competitive enzyme-linked immunosorbent assay (ELISA). None of the samples was contaminated with aflatoxins. The most predominant mycotoxin was ochratoxin A with the mean level of 4.84 ? 4.49 ppb in 19.7% of the examined samples. Zearalenone, fumonisins, and deoxynivalenol were found in 9.21, 14.5 and 3.9% of the samples, respectively. Mycotoxin content in the investigated samples was compared with the regulations of Serbia and those of the European Union.

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