Association of udder traits with single nucleotide polymorphisms in crossbred Bos indicus–Bos taurus cows1,2

Recent evidence suggests that several cattle breeds may be more resistant to infection with the zoonotic pathogen Mycobacterium bovis than others. Our data presented here suggests that the response to mycobacterial antigens varies in macrophages generated from Brown Swiss (BS) and Holstein Frisian (HF) cattle, two breeds belonging to the Bos taurus family. Whole genome sequencing of the Brown Swiss genome identified several potential candidate genes, in particular Toll-like Receptor-2 (TLR2) a pattern recognition receptor (PRR) that has previously been described to be involved in mycobacterial recognition. Further investigation revealed single nucleotide polymorphisms (SNP) in TLR2 that were identified between DNA isolated from cells of BS and HF cows. Interestingly, one specific SNP, H326Q, showed a different genotype frequency in two cattle subspecies, Bos taurus and Bos indicus. Cloning of the TLR2 gene and subsequent gene-reporter and chemokine assays revealed that this SNP, present in BS and Bos indicus breeds, resulted in a significantly higher response to mycobacterial antigens as well as tri-acylated lipopeptide ligands in general. Comparing wild-type and H326Q containing TLR2 responses, wild-type bovine TLR2 response showed clear, diminished mycobacterial antigen responses compared to human TLR2, however bovine TLR2 responses containing H326Q were found to be partially recovered compared to human TLR2. The creation of human:bovine TLR2 chimeras increased the response to mycobacterial antigens compared to the full-length bovine TLR2, but significantly reduced the response compared to the full-length human TLR2. Thus, our data, not only present evidence that TLR2 is a major PRR in the mammalian species-specific response to mycobacterial antigens, but furthermore, that there are clear differences between the response seen in different cattle breeds, which may contribute to their enhanced or reduced susceptibility to mycobacterial infection.

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Identification and annotation of breed-specific single nucleotide polymorphisms in Bos taurus genomes

PLoS ONE ◽

10.1371/journal.pone.0198419 ◽

2018 ◽

Vol 13 (6) ◽

pp. e0198419 ◽

Cited By ~ 2

Author(s):

Bartosz Czech ◽

Magdalena Frąszczak ◽

Magda Mielczarek ◽

Joanna Szyda

Keyword(s):

Single Nucleotide Polymorphisms ◽

Bos Taurus ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle1,2

Journal of Animal Science ◽

10.2527/2005.83113x ◽

2005 ◽

Vol 83 (1) ◽

pp. 13-19 ◽

Cited By ~ 90

Author(s):

E. Casas ◽

S. N. White ◽

D. G. Riley ◽

T. P. L. Smith ◽

R. A. Brenneman ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Bos Indicus ◽

Carcass Composition ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle

Meat Science ◽

10.1016/j.meatsci.2010.02.025 ◽

2010 ◽

Vol 85 (3) ◽

pp. 515-518 ◽

Cited By ~ 41

Author(s):

L. Pannier ◽

A.M. Mullen ◽

R.M. Hamill ◽

P.C. Stapleton ◽

T. Sweeney

Keyword(s):

Single Nucleotide Polymorphisms ◽

Association Analysis ◽

Bos Taurus ◽

Intramuscular Fat ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

Frontiers in Immunology ◽

10.3389/fimmu.2021.764390 ◽

2021 ◽

Vol 12 ◽

Author(s):

Marie-Christine Bartens ◽

Amanda J. Gibson ◽

Graham J. Etherington ◽

Federica Di Palma ◽

Angela Holder ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Bos Indicus ◽

Full Length ◽

Nucleotide Polymorphisms ◽

Wild Type ◽

Single Nucleotide ◽

Brown Swiss ◽

Cattle Breeds ◽

Species Specific ◽

Mycobacterial Antigens

Recent evidence suggests that several cattle breeds may be more resistant to infection with the zoonotic pathogen Mycobacterium bovis. Our data presented here suggests that the response to mycobacterial antigens varies in macrophages generated from Brown Swiss (BS) and Holstein Friesian (HF) cattle, two breeds belonging to the Bos taurus family. Whole genome sequencing of the Brown Swiss genome identified several potential candidate genes, in particular Toll-like Receptor-2 (TLR2), a pattern recognition receptor (PRR) that has previously been described to be involved in mycobacterial recognition. Further investigation revealed single nucleotide polymorphisms (SNP) in TLR2 that were identified between DNA isolated from cells of BS and HF cows. Interestingly, one specific SNP, H326Q, showed a different genotype frequency in two cattle subspecies, Bos (B.) taurus and Bos indicus. Cloning of the TLR2 gene and subsequent gene-reporter and chemokine assays revealed that this SNP, present in BS and Bos indicus breeds, resulted in a significantly higher response to mycobacterial antigens as well as tri-acylated lipopeptide ligands in general. Comparing wild-type and H326Q containing TLR2 responses, wild-type bovine TLR2 response showed clear, diminished mycobacterial antigen responses compared to human TLR2, however bovine TLR2 responses containing H326Q were found to be partially recovered compared to human TLR2. The creation of human:bovine TLR2 chimeras increased the response to mycobacterial antigens compared to the full-length bovine TLR2, but significantly reduced the response compared to the full-length human TLR2. Thus, our data, not only present evidence that TLR2 is a major PRR in the mammalian species-specific response to mycobacterial antigens, but furthermore, that there are clear differences between the response seen in different cattle breeds, which may contribute to their enhanced or reduced susceptibility to mycobacterial infection.

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Effect of single nucleotide polymorphisms ofCAPN1andCASTgenes on meat traits in Nellore beef cattle (Bos indicus) and in their crosses withBos taurus

Animal Genetics ◽

10.1111/j.1365-2052.2009.01859.x ◽

2009 ◽

Vol 40 (4) ◽

pp. 456-462 ◽

Cited By ~ 39

Author(s):

R. A. Curi ◽

L. A. L. Chardulo ◽

M. C. Mason ◽

M. D. B. Arrigoni ◽

A. C. Silveira ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Beef Cattle ◽

Bos Indicus ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Analysis of population differentiation in North Eurasian cattle (Bos taurus) using single nucleotide polymorphisms in three genes associated with production traits

Animal Genetics ◽

10.1111/j.1365-2052.2006.01479.x ◽

2006 ◽

Vol 37 (4) ◽

pp. 390-392 ◽

Cited By ~ 12

Author(s):

M. H. Li ◽

T. Adamowicz ◽

M. Switonski ◽

I. Ammosov ◽

Z. Ivanova ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Population Differentiation ◽

Bos Taurus ◽

Nucleotide Polymorphisms ◽

Production Traits ◽

Single Nucleotide

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Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2012000200019 ◽

2012 ◽

Vol 47 (2) ◽

pp. 294-301 ◽

Cited By ~ 6

Author(s):

Rogério Abdallah Curi ◽

Monique Marcondes Krauskopf ◽

Janaína Conte Hadlich ◽

Marina Rufino Salinas Fortes ◽

Dianne Margaret Vankan ◽

...

Keyword(s):

Allele Frequency ◽

Multiple Testing ◽

Bos Taurus ◽

Bos Indicus ◽

Total Sample ◽

Candidate Snps ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Fragmentation Index ◽

Allele Substitution

The objective of this work was to evaluate the effects of single-nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non-informative in Nelore animals. In crossbred animals, the IGF1 C allele was associated with greater REA. However, this relation was not significant after Bonferroni correction for multiple testing. The A allele of the MSTN polymorphism was absent in Nelore cattle and was only found in two crossbred animals. The polymorphisms of MYOD1 and MYF5 were little informative in Nelore animals with G allele frequency of 0.097 and A allele frequency of 0.031, respectively. These markers show no association with the analyzed traits in the total sample of evaluated animals.

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