In Vitro Metabolism of Formononetin and Biochanin a in Bovine Rumen Fluid

The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was isolated and then amplified in vitro through the PCR method. Determination of nucleotide sequence of 16S rDNA was performed with sequencing method. The result of nucleotide sequence was than compared with GenBank database. The BLAST was then applied to identify the phylogenetic tree. Based on the biochemical characterization and nucleotide sequences, that isolate was identified as Lactobacillus rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp TG15 showed viability bacteria in MRSA as control as much as 1.1 x 108 CFU/ml, mean while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 x 106 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 x 107 CFU/ml). Index antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus subsp TG15 and that isolate has ability as the probiotic candidate. Keywords: L.rhamnosus subsp TG15; survival on acidity; bile salts; S.aureus and E.coli

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In Vitro Inactivation of Escherichia coli O157:H7 in Bovine Rumen Fluid by Caprylic Acid

Journal of Food Protection ◽

10.4315/0362-028x-67.5.884 ◽

2004 ◽

Vol 67 (5) ◽

pp. 884-888 ◽

Cited By ~ 26

Author(s):

THIRUNAVUKKARASU ANNAMALAI ◽

MANOJ KUMAR MOHAN NAIR ◽

PATRICK MAREK ◽

PRADEEP VASUDEVAN ◽

DAVID SCHREIBER ◽

...

Keyword(s):

Escherichia Coli ◽

Caprylic Acid ◽

Rumen Fluid ◽

Bacterial Load ◽

Anaerobic Bacteria ◽

Inhibitory Effect ◽

Escherichia Coli O157 ◽

E Coli ◽

Bovine Rumen

The antibacterial effect of caprylic acid (35 and 50 mM) on Escherichia coli O157:H7 and total anaerobic bacteria at 39° C in rumen fluid (pH 5.6 and 6.8) from 12 beef cattle was investigated. The treatments containing caprylic acid at both pHs significantly reduced (P < 0.05) the population of E. coli O157:H7 compared with that in the control samples. At pH 5.6, both levels of caprylic acid killed E. coli O157:H7 rapidly, reducing the pathogen population to undetectable levels at 1 min of incubation (a more than 6.0-log CFU/ml reduction). In buffered rumen fluid at pH 6.8, 50 mM caprylic acid reduced the E. coli O157:H7 population to undetectable levels at 1 min of incubation, whereas 35 mM caprylic acid reduced the pathogen by approximately 3.0 and 5.0 log CFU/ml at 8 and 24 h of incubation, respectively. At both pHs, caprylic acid had a significantly lesser (P < 0.05) and minimal inhibitory effect on the population of total anaerobic bacteria in rumen compared with that on E. coli O157:H7. At 24 h of incubation, caprylic acid (35 and 50 mM) reduced the population of total anaerobic bacteria by approximately 2.0 log CFU/ml at pH 5.6, whereas at pH 6.8, caprylic acid (35 mM) did not have any significant (P > 0.05) inhibitory effect on total bacterial load. Results of this study revealed that caprylic acid was effective in inactivating E. coli O157:H7 in bovine rumen fluid, thereby justifying its potential as a preslaughter dietary supplement for reducing pathogen carriage in cattle.

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Acetylation/deacetylation reactions of T-2, acetyl T-2, HT-2, and acetyl HT-2 toxins in bovine rumen fluid in vitro

Journal of Agricultural and Food Chemistry ◽

10.1021/jf00075a018 ◽

1987 ◽

Vol 35 (3) ◽

pp. 354-358 ◽

Cited By ~ 12

Author(s):

Craig E. Munger ◽

G. Wayne Ivie ◽

Ronald J. Christopher ◽

Bruce D. Hammock ◽

Timothy D. Phillips

Keyword(s):

Rumen Fluid ◽

Bovine Rumen

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Action of Rumen Fluid on Pesticides, In Vitro Destruction of Some Organophosphate Pesticides by Bovine Rumen Fluid

Journal of Agricultural and Food Chemistry ◽

10.1021/jf60081a007 ◽

1957 ◽

Vol 5 (11) ◽

pp. 859-863 ◽

Cited By ~ 44

Author(s):

J. W. Cook

Keyword(s):

Rumen Fluid ◽

Organophosphate Pesticides ◽

Bovine Rumen

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Comprehensive Study of the in Vivo and in Vitro Metabolism of Dietary Isoflavone Biochanin A Based on UHPLC-Q-TOF-MS/MS

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.9b05776 ◽

2019 ◽

Vol 67 (45) ◽

pp. 12481-12495 ◽

Cited By ~ 1

Author(s):

Jintuo Yin ◽

Xiaowei Zhang ◽

Yuqian Zhang ◽

Yinling Ma ◽

Luya Li ◽

...

Keyword(s):

In Vitro Metabolism ◽

Biochanin A ◽

Tof Ms ◽

Comprehensive Study

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METABOLISM OF ALIPHATIC NITRO COMPOUNDS IN BOVINE RUMEN FLUID

Canadian Journal of Animal Science ◽

10.4141/cjas80-041 ◽

1980 ◽

Vol 60 (2) ◽

pp. 319-325 ◽

Cited By ~ 24

Author(s):

W. MAJAK ◽

L. J. CLARK

Keyword(s):

Nitro Compound ◽

Clearance Rate ◽

Rumen Fluid ◽

Nitro Compounds ◽

Aliphatic Nitro Compounds ◽

Bovine Rumen ◽

Nitropropionic Acid ◽

3 Nitropropionic Acid

Metabolic studies with rumen fluid were conducted to determine rates of disappearance for 3-nitropropanol (3NPOH) and 3-nitropropionic acid (NPA), forage constituents which can be toxic to ruminants and non-ruminants. The in vitro rate of disappearance for NPA was substantially greater (P < 0.01) than for 3NPOH. The nitro compounds were metabolized most effectively when the inoculum originated from cattle fed orchardgrass soilage as compared with orchardgrass hay, alfalfa soilage or alfalfa hay. The in vivo ruminai clearance rate was substantially greater (P < 0.05) for 3NPHO than for 2-nitropropanol, a nitro compound which was not metabolized in vitro and this would be predicted if metabolism in the rumen contributed to the rate of 3NPOH disappearance.

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