PI3K signaling Pathway gene expression analysis in cumulus cells by Real-Time PCR

2016 ◽  
Author(s):  
Paolo Giovanni Artini
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Signaling Pathway ◽  
Real Time Pcr ◽  
Gene Expression Analysis ◽  
Cumulus Cells ◽  
Pi3k Signaling ◽  
Pathway Gene ◽  
Pi3k Signaling Pathway ◽  
Signaling Pathway Gene

Gene expression analysis of antimony resistance in Leishmania tropica using quantitative real-time PCR focused on genes involved in trypanothione metabolism and drug transport

2018 ◽  
Vol 311 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Mehdi Mohebali ◽  
Elham Kazemirad ◽  
Homa Hajjaran ◽  
Elaheh Kazemirad ◽  
Mohammad Ali Oshaghi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Drug Transport ◽  
Gene Expression Analysis ◽  
Leishmania Tropica ◽  
Quantitative Real Time Pcr ◽  
Antimony Resistance

scholarly journals Embryonic stem cell microenvironment enhances proliferation of human retinal pigment epithelium cells by activating the PI3K signaling pathway

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiahui Liu ◽  
Liu Yang ◽  
Xiaoran Wang ◽  
Shoubi Wang ◽  
Zheqian Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Signaling Pathway ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Embryonic Stem ◽  
Age Related Macular Degeneration ◽  
Pi3k Signaling ◽  
Age Related ◽  
Pi3k Signaling Pathway

Abstract Background Retinal pigment epithelium (RPE) replacement has been proposed as an efficacious treatment for age-related macular degeneration (AMD), which is the primary cause of vision loss in the elderly worldwide. The embryonic stem cell (ESC) microenvironment has been demonstrated to enable mature cells to gain a powerful proliferative ability and even enhance the stem/progenitor phenotype via activation of the phosphoinositide 3-kinase (PI3K) signaling pathway. As the PI3K signaling pathway plays a pivotal role in proliferation and homeostasis of RPE, we hypothesize that the stemness and proliferative capability of RPE can be enhanced by the ESC microenvironment via activation of the PI3K signaling pathway. Methods To investigate whether the ESC microenvironment improves the stem cell phenotype and proliferation properties of human RPE (hRPE) cells by regulating the PI3K signaling pathway, primary hRPE cells were cocultured with either ESCs or human corneal epithelial cells (CECs) for 72 h, after which their proliferation, apoptosis, cell cycle progression, and colony formation were assayed to evaluate changes in their biological characteristics. Gene expression was detected by real-time PCR and protein levels were determined by western blotting or immunofluorescence. LY294002, an antagonist of the PI3K signaling pathway, was used to further confirm the mechanism involved. Results In comparison to hRPE cells cultured alone, hRPE cells cocultured with ESCs had an increased proliferative capacity, reduced apoptotic rate, and higher colony-forming efficiency. The expression of the stem cell-associated marker KLF4 and the differentiation marker CRALBP increased and decreased, respectively, in hRPE cells isolated from the ESC coculture. Furthermore, PI3K pathway-related genes were significantly upregulated in hRPE cells after exposure to ESCs. LY294002 reversed the pro-proliferative effect of ESCs on hRPE cells. In contrast, CECs did not share the ability of ESCs to influence the biological behavior and gene expression of hRPE cells. Conclusions Our findings indicate that the ESC microenvironment enhances stemness and proliferation of hRPE cells, partially via activation of the PI3K signaling pathway. This study may have a significant impact and clinical implication on cell therapy in regenerative medicine, specifically for age-related macular degeneration.

How to do successful gene expression analysis using real-time PCR

Methods ◽  
2010 ◽  
Vol 50 (4) ◽  
pp. 227-230 ◽  
Author(s):  
Stefaan Derveaux ◽  
Jo Vandesompele ◽  
Jan Hellemans
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Gene Expression Analysis

Comparative Gene Expression Analysis of Bovine Nuclear-Transferred Embryos with Different Developmental Potential by cDNA Microarray and Real-Time PCR to Determine Genes That Might Reflect Calf Normality

2007 ◽  
Vol 9 (4) ◽  
pp. 495-511 ◽  
Author(s):  
Yoko Kato ◽  
Xiangping Li ◽  
Dasari Amarnath ◽  
Koichi Ushizawa ◽  
Kazuyoshi Hashizume ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Cdna Microarray ◽  
Real Time Pcr ◽  
Gene Expression Analysis ◽  
Developmental Potential

TGFbeta-BMP signaling pathway gene expression in osteoporotic postmenopausal women by DNA microarrays

Bone ◽  
2011 ◽  
Vol 48 ◽  
pp. S159
Author(s):  
T. Koromila ◽  
Z. Dailiana ◽  
S. Samara ◽  
C. Chassanidis ◽  
V. Aleporou - Marinou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Postmenopausal Women ◽  
Signaling Pathway ◽  
Dna Microarrays ◽  
Bmp Signaling ◽  
Pathway Gene ◽  
Signaling Pathway Gene
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