Embryo development after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes

2016 ◽  
Author(s):  
Ioannis Sfontouris
Keyword(s):  
Continuous Culture ◽  
Embryo Development ◽  
Randomized Study ◽  
Single Step ◽  
Prospective Randomized Study ◽  
Sibling Oocytes

scholarly journals Blastocyst utilization rates after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes

2017 ◽  
Vol 34 (10) ◽  
pp. 1377-1383 ◽  
Author(s):  
Ioannis A. Sfontouris ◽  
Efstratios M. Kolibianakis ◽  
George T. Lainas ◽  
Christos A. Venetis ◽  
George K. Petsas ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Randomized Study ◽  
Single Step ◽  
Prospective Randomized Study ◽  
Sibling Oocytes

Autologous embryo–cumulus cells co-culture and blastocyst transfer in repeated implantation failures: a collaborative prospective randomized study

Zygote ◽  
2011 ◽  
Vol 20 (2) ◽  
pp. 173-180 ◽  
Author(s):  
M. Benkhalifa ◽  
A. Demirol ◽  
T. Sari ◽  
E. Balashova ◽  
M. Tsouroupaki ◽  
...  
Keyword(s):  
Embryo Development ◽  
Randomized Study ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Prospective Randomized Study ◽  
Human Embryos ◽  
Feeder Cells ◽  
Positive Role ◽  
Significant Difference

SummaryIn repeated implantation failure, the co-culture of human embryos with somatic cells has been reported to promote the improvement of embryos quality, implantation and pregnancy rate. It was reported that feeder cells can be more beneficial to the oocyte and embryo by detoxifying the culture medium and supporting embryo development via different pathways. In this study, 432 patients, each with a minimum of three repeated implantation failures, were accepted for a prospective randomized study with or without autologous cumulus cell embryo co-culture and transfer at day 3 or day 5–6. We also investigated the expression of leukaemia inhibitor factor (LIF) and platelet activating factor receptor (PAF-R) on day 3 confluent cumulus cells. The statistic analysis of the data showed significant difference of implantation and clinical pregnancy rates between classical culture and day 3 compared with co-culture and day 5–6 transfer. The molecular analysis showed that cumulus cells express the LIF and the PAF-R genes and confirmed the possible positive role of growth factors and cytokines in early embryo development. Embryo co-culture systems with autologous cells can be beneficial in routine in vitro fertilization for embryo selection and implantation improvement. More molecular investigations need to be done to improve elucidation of the complex dialogue between the embryo and feeder cells prior to implantation and to understand the involved biological function and molecular process during embryo development.

scholarly journals OUTCOMES OF OOCYTES ICSI’D WITH SPERM PROCESSED WITH EITHER DENSITY GRADIENT PREPERATION OR THE ZYMOT PREPERATION DEVICE; A PROSPECTIVE RANDOMIZED STUDY UTILIZING SIBLING OOCYTES

2021 ◽  
Vol 116 (3) ◽  
pp. e42
Author(s):  
Tyl H. Taylor ◽  
Jennifer L. Patrick ◽  
Jessica Nicole Manns ◽  
Joe Whelan ◽  
Allison Clayborne ◽  
...  
Keyword(s):  
Density Gradient ◽  
Randomized Study ◽  
Prospective Randomized Study ◽  
Sibling Oocytes

1374: A Two Years Follow-Up, Prospective Randomized Study on Cystocele Repair with or without Pelvicol Implant

2007 ◽  
Vol 177 (4S) ◽  
pp. 453-453 ◽  
Author(s):  
Ervin Kocjancic ◽  
Simone Crivellaro ◽  
Fabio Bernasconi ◽  
Fabio Magatti ◽  
Bruno Frea ◽  
...  
Keyword(s):  
Randomized Study ◽  
Prospective Randomized Study

532: Intravesical Resiniferatoxin Versus Botulinum-A Toxin Injections in Patients with Neurogenic Detrusor Overactivity: A Prospective Randomized Study

2004 ◽  
Vol 171 (4S) ◽  
pp. 142-142 ◽  
Author(s):  
Antonella Giannantoni ◽  
Savino M. Di Stasi ◽  
Robert L. Stephen ◽  
Gerardo Pizzirusso ◽  
Ettore Mearini ◽  
...  
Keyword(s):  
Detrusor Overactivity ◽  
Randomized Study ◽  
Prospective Randomized Study ◽  
Neurogenic Detrusor Overactivity ◽  
Botulinum A Toxin
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