scholarly journals 5 / Polymerase chain reaction (PCR) diagnosis and identification of mucormycosis in patients with suspected invasive fungal infection

2018 ◽  
Author(s):  
T. Shokohi
Keyword(s):  
Polymerase Chain Reaction ◽  
Fungal Infection ◽  
Invasive Fungal Infection ◽  
Chain Reaction ◽  
Pcr Diagnosis ◽  
Polymerase Chain

scholarly journals Optimization of Polymerase Chain Reaction for the Detection of Borrelia Burgdorferi in Biologic Specimens

1993 ◽  
Vol 5 (4) ◽  
pp. 548-554 ◽  
Author(s):  
Abigail C. Kaufman ◽  
Craig E. Greene ◽  
Royal A. McGraw
Keyword(s):  
Polymerase Chain Reaction ◽  
Borrelia Burgdorferi ◽  
Base Pair ◽  
Ethylenediaminetetraacetic Acid ◽  
Chromosomal Dna ◽  
Chain Reaction ◽  
Clinical Specimens ◽  
Pcr Diagnosis ◽  
Blood And Urine Samples ◽  
Polymerase Chain

This study describes the use of a newly constructed set of primers that amplifies an U-base pair (bp) segment of Borrelia burgdorferi chromosomal DNA. This 85-bp product is not produced when other Borrelia species, Leptospira, or other bacteria are subjected to polymerase chain reaction (PCR). We also describe a rapid method of optimizing the amplification of B. burgdorferi DNA from canine ethylenediaminetetraacetic acid-treated blood and urine samples that circumvents some of the problems encountered due to low number of spirochetes in clinical specimens and that removes inhibiting substances, which improves the PCR diagnosis of canine Lyme borreliosis.

The contribution of PCR testing to influenza and pertussis notifications in Australia

2015 ◽  
Vol 144 (2) ◽  
pp. 306-314 ◽  
Author(s):  
M. C. KACZMAREK ◽  
R. S. WARE ◽  
S. B. LAMBERT
Keyword(s):  
Polymerase Chain Reaction ◽  
Surveillance System ◽  
Diagnostic Methods ◽  
Chain Reaction ◽  
Notifiable Diseases ◽  
Pcr Diagnosis ◽  
Polymerase Chain

SUMMARYInfluenza and pertussis are the two most common vaccine-preventable infections notified in Australia. We assessed the role of polymerase chain reaction (PCR) diagnosis in influenza and pertussis cases notified to the Australian National Notifiable Diseases Surveillance System (NNDSS). There were a total of 2 10 786 notified influenza cases (2001–2013) and 2 55 866 notified pertussis cases (1991–2013). After 1 January 2007, the majority of influenza and pertussis notifications were PCR-based (80·5% and 59·6%, respectively). Before 31 December 2006, PCR-based notifications were limited (29·1% and 11·7%, respectively). By 2013, PCR-based notifications had largely replaced all other diagnostic methods, with the exception of serology-based notifications in pertussis cases in adults aged ⩾25 years.

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