Bacterial colonization and antibiotic resistance in patients with atopic dermatitis

Background. The aim of this study was to assess the antibiotic resistance of S. aureus isolated from the skin of atopic dermatitis (AD) children. Materials and methods. 256 AD children were included in the study in 2014-2016. Microbiological examination of skin scraping and determination of antibiotics sensitivity were performed. Results. Staphylococcus aureus was isolated in 173 (67,6%) of 256 patients. Resistance to erythromycin was established in 64,6 and 74,6%, respectively. Retrospective analyses showed that S. aureus became more sensitive to ampicillin, ceftriaxone, ciprofloxacin but more resistant to erythromycin. Conclusion. Ceftriaxone and cefoxitin but not erythromycin and ampicillin are the drugs of choice for the treatment of atopic dermatitis complicated by secondary infection in children.

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Identifying Genetic Determinants of Atopic Dermatitis and Bacterial Colonization Using Whole Genome Sequencing

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2014.12.1903 ◽

2015 ◽

Vol 135 (2) ◽

pp. AB391 ◽

Cited By ~ 2

Author(s):

Rasika A. Mathias ◽

Sameer Chavan ◽

Kruthika R. Iyer ◽

Nicholas M. Rafaels ◽

Meher Boorgula ◽

...

Keyword(s):

Atopic Dermatitis ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Bacterial Colonization ◽

Whole Genome ◽

Genetic Determinants

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In Vitro Evaluation of Gentamicin or Vancomycin Containing Bone Graft Substitute in the Prevention of Orthopedic Implant-Related Infections

International Journal of Molecular Sciences ◽

10.3390/ijms21239250 ◽

2020 ◽

Vol 21 (23) ◽

pp. 9250

Author(s):

Alessandro Bidossi ◽

Marta Bottagisio ◽

Nicola Logoluso ◽

Elena De Vecchi

Keyword(s):

Antibiotic Resistance ◽

Bone Graft ◽

Calcium Sulfate ◽

Prolonged Exposure ◽

Bacterial Colonization ◽

Bone Graft Substitute ◽

Bacterial Strains ◽

Bone Graft Substitutes ◽

Biofilm Development

Antibiotic-loaded bone graft substitutes are attractive clinical options and have been used for years either for prophylaxis or therapy for periprosthetic and fracture-related infections. Calcium sulfate and hydroxyapatite can be combined in an injectable and moldable bone graft substitute that provides dead space management with local release of high concentrations of antibiotics in a one-stage approach. With the aim to test preventive strategies against bone infections, a commercial hydroxyapatite/calcium sulfate bone graft substitute containing either gentamicin or vancomycin was tested against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa, harboring different resistance determinants. The prevention of bacterial colonization and biofilm development by selected microorganisms was investigated along with the capability of the eluted antibiotics to select for antibiotic resistance. The addition of antibiotics drastically affected the ability of the selected strains to adhere to the tested compound. Furthermore, both the antibiotics eluted by the bone graft substitutes were able to negatively impair the biofilm maturation of all the staphylococcal strains. As expected, P. aeruginosa was significantly affected only by the gentamicin containing bone graft substitutes. Finally, the prolonged exposure to antibiotic-containing sulfate/hydroxyapatite discs did not lead to any stable or transient adaptations in either of the tested bacterial strains. No signs of the development of antibiotic resistance were found, which confirms the safety of this strategy for the prevention of infection in orthopedic surgery.

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Prevalence of Staphylococcus aureus and antibiotic resistance in children with atopic dermatitis in Arar, Saudi Arabia

Journal of Dermatology & Dermatologic Surgery ◽

10.1016/j.jssdds.2013.11.001 ◽

2014 ◽

Vol 18 (1-2) ◽

pp. 22-26 ◽

Cited By ~ 1

Author(s):

Dhaifallah A. Alenizi

Keyword(s):

Staphylococcus Aureus ◽

Atopic Dermatitis ◽

Saudi Arabia ◽

Antibiotic Resistance

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Pearls and pitfalls of bathing in atopic dermatitis

Allergy and Asthma Proceedings ◽

10.2500/aap.2019.40.4210 ◽

2019 ◽

Vol 40 (3) ◽

pp. 204-206

Author(s):

Pasquale Comberiati ◽

Luca Pecoraro ◽

Roberta Pigozzi ◽

Diego Peroni ◽

Giorgio Piacentini

Keyword(s):

Atopic Dermatitis ◽

American Academy ◽

Maintenance Treatment ◽

Bacterial Colonization ◽

Skin Care ◽

Bath Water ◽

Nonpharmacologic Intervention ◽

Term Maintenance ◽

Review Current

The latest guidelines of the American Academy of Dermatology suggest bathing as an advantageous nonpharmacologic intervention for regular skin care of patients with atopic dermatitis. Regular bathing with water can hydrate the skin, remove scale, crust, irritants, and allergens, enhance penetration of topical agents, and potentially reduce bacterial colonization. However, it is still unclear whether the addition of oils, emollients, and other additives to bath water may produce further benefits for the management of atopic dermatitis. This article will review current pearls and pitfalls of adding salts and nonirritating, nonsensitizing substances to water baths for the long-term maintenance treatment of atopic dermatitis in children.

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Bactericidal and Biocompatible Properties of Plasma Chemical Oxidized Titanium (TiOB®) with Antimicrobial Surface Functionalization

Materials ◽

10.3390/ma12060866 ◽

2019 ◽

Vol 12 (6) ◽

pp. 866 ◽

Cited By ~ 3

Author(s):

Stefan Kranz ◽

André Guellmar ◽

Andrea Voelpel ◽

Tobias Lesser ◽

Silke Tonndorf-Martini ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibiotic Resistance ◽

Surface Functionalization ◽

Bacterial Colonization ◽

Positive Control ◽

Plasma Chemical ◽

Functionalized Surfaces ◽

Bactericidal Properties ◽

Major Interest ◽

Bactericidal Effects

Coating of plasma chemical oxidized titanium (TiOB®) with gentamicin-tannic acid (TiOB® gta) has proven to be efficient in preventing bacterial colonization of implants. However, in times of increasing antibiotic resistance, the development of alternative antimicrobial functionalization strategies is of major interest. Therefore, the aim of the present study is to evaluate the antibacterial and biocompatible properties of TiOB® functionalized with silver nanoparticles (TiOB® SiOx Ag) and ionic zinc (TiOB® Zn). Antibacterial efficiency was determined by agar diffusion and proliferation test on Staphylocuccus aureus. Cytocompatibility was analyzed by direct cultivation of MC3T3-E1 cells on top of the functionalized surfaces for 2 and 4 d. All functionalized surfaces showed significant bactericidal effects expressed by extended lag phases (TiOB® gta for 5 h, TiOB® SiOx Ag for 8 h, TiOB® Zn for 10 h). While TiOB® gta (positive control) and TiOB® Zn remained bactericidal for 48 h, TiOB® SiOx Ag was active for only 4 h. After direct cultivation for 4 d, viable MC3T3-E1 cells were found on all surfaces tested with the highest biocompatibility recorded for TiOB® SiOx Ag. The present study revealed that functionalization of TiOB® with ionic zinc shows bactericidal properties that are comparable to those of a gentamicin-containing coating.

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