Morphologic status and angulation of meiotic spindle in vitrification-warmed oocytes of donor bank: effects on the ICSI outcomes

2018 ◽  
Author(s):  
Yao Cheng Huang ◽  
Tzu-Hsuan Chuang ◽  
Meng Ju Lee
Keyword(s):  
Meiotic Spindle ◽  
Bank Effects ◽  
Icsi Outcomes

scholarly journals Effects of maternal ageing on ICSI outcomes and embryo development in relation to oocytes morphological characteristics of birefringent structures

Zygote ◽  
2014 ◽  
Vol 23 (4) ◽  
pp. 550-555 ◽  
Author(s):  
Cem Korkmaz ◽  
Yesim Bayoglu Tekin ◽  
Mehmet Sakinci ◽  
Cihangir Mutlu Ercan
Keyword(s):  
Embryo Development ◽  
Morphological Characteristics ◽  
Oocyte Quality ◽  
Fertilization Success ◽  
Meiotic Spindle ◽  
Poor Responders ◽  
Polarization Microscopy ◽  
Quantitative Measurements ◽  
Icsi Outcomes

SummaryThe aim of this study was to determine the morphological characteristics of the older reproductive aged women's oocytes and to reveal the influence of these characteristics on intra-cytoplasmic sperm injection (ICSI) outcomes. The oocytes of women older than 35 years of age were evaluated retrospectively. Non-invasive polarization microscopy (PolScope) examinations of mature oocytes were performed by measurement of meiotic spindles’ length, area and retardance and zona pellucida thickness and retardance. Fertilization and conception competence and the correlation with the birefringent structures were assessed. Two hundred and thirteen mature oocytes from 54 women were evaluated with a PolScope. Length of the meiotic spindle was shown to be related to fertilization success of women with advanced maternal age. In conclusion, the PolScope is a useful device used to identify the oocyte quality. Quantitative measurements of meiotic spindle parameters may be valuable for the selection of high-quality oocytes that have the potential for embryo development in the in vitro fertilization (IVF) laboratory of women older than 35 years of age who are mostly poor responders.

Zona pellucida birefringence and meiotic spindle visualization as predictors of ICSI outcomes. Preliminary results

2010 ◽  
Vol 94 (4) ◽  
pp. S136
Author(s):  
M.I. Riqueros Arévalo ◽  
E. Bonilla Egea ◽  
M. Esbert Algam ◽  
A. Pellicer Martínez ◽  
A. Ballesteros Boluda ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Meiotic Spindle ◽  
Preliminary Results ◽  
Icsi Outcomes

scholarly journals Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and ICSI outcomes

2009 ◽  
Vol 18 (5) ◽  
pp. 681-686 ◽  
Author(s):  
Camila Madaschi ◽  
Tsutomu Aoki ◽  
Daniela Paes de Almeida Ferreira Braga ◽  
Rita de Cássia Sávio Figueira ◽  
Luciana Semião Francisco ◽  
...  
Keyword(s):  
Embryo Development ◽  
Zona Pellucida ◽  
Meiotic Spindle ◽  
Icsi Outcomes

scholarly journals Meiotic spindle presence and oocyte morphology do not predict clinical ICSI outcomes: a study of 967 transferred embryos

2006 ◽  
Vol 13 (5) ◽  
pp. 661-667 ◽  
Author(s):  
S Chamayou ◽  
C Ragolia ◽  
C Alecci ◽  
G Storaci ◽  
E Maglia ◽  
...  
Keyword(s):  
Meiotic Spindle ◽  
Oocyte Morphology ◽  
Icsi Outcomes

scholarly journals Relationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: a meta-analysis

2009 ◽  
Vol 18 (2) ◽  
pp. 235-243 ◽  
Author(s):  
CG Petersen ◽  
JBA Oliveira ◽  
AL Mauri ◽  
FC Massaro ◽  
RLR Baruffi ◽  
...  
Keyword(s):  
Meta Analysis ◽  
Meiotic Spindle ◽  
Human Oocytes ◽  
Icsi Outcomes

Probing the ultrastructure of the mitotic and meiotic spindle in eggs: An expeditious approach based on semi-thin (0.25 μm) serial sections

1983 ◽  
Vol 41 ◽  
pp. 552-555
Author(s):  
Conly L. Rieder ◽  
S. Bowser ◽  
R. Nowogrodzki ◽  
K. Ross ◽  
G. Sluder
Keyword(s):  
Small Sample Size ◽  
Small Sample ◽  
Meiotic Spindle ◽  
Serial Sections ◽  
Mitotic Apparatus ◽  
Thin Sections ◽  
Cell Cycles ◽  
Ultrastructural Studies

Eggs have long been a favorite material for studying the mechanism of karyokinesis in-vivo and in-vitro. They can be obtained in great numbers and, when fertilized, divide synchronously over many cell cycles. However, they are not considered to be a practical system for ultrastructural studies on the mitotic apparatus (MA) for several reasons, the most obvious of which is that sectioning them is a formidable task: over 1000 ultra-thin sections need to be cut from a single 80-100 μm diameter egg and of these sections only a small percentage will contain the area or structure of interest. Thus it is difficult and time consuming to obtain reliable ultrastructural data concerning the MA of eggs; and when it is obtained it is necessarily based on a small sample size.We have recently developed a procedure which will facilitate many studies concerned with the ultrastructure of the MA in eggs. It is based on the availability of biological HVEM's and on the observation that 0.25 μm thick serial sections can be screened at high resolution for content (after mounting on slot grids and staining with uranyl and lead) by phase contrast light microscopy (LM; Figs 1-2).

Quantitative ultrastructural reconstruction of small regions within large volumes by correlative light and high voltage electron microscopy of serial 0.25-0.50 μm sections

1987 ◽  
Vol 45 ◽  
pp. 578-581
Author(s):  
Conly L. Rieder ◽  
Frederick J. Miller ◽  
Edwin Davison ◽  
Samuel S. Bowser ◽  
Kirsten Lewis ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Mitotic Spindle ◽  
Meiotic Spindle ◽  
High Voltage Electron Microscopy ◽  
Male And Female ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Differential Stability ◽  
Sperm Aster

In this abstract we Illustrate how same-section correlative light and high voltage electron microscopy (HVEM) of serial 0.25-0.50-μm sections can answer questions which are difficult to approach by EM of 60-100 nm sections.Starfish (Pisaster and Asterlas) eggs are fertilized at meiosis I when the oocyte contains two maternal centrosomes (e.g., asters) which form the poles of the first meiotic spindle. Immediately after fertilization a sperm aster is assembled in the vicinity of the male pronucleus and persists throughout meiosis. At syngamy the sperm aster splits to form the poles of the first mitotic spindle. During this time the functional and replicative properties of the maternal centrosome, inherited from the last meiotic division, are lost. The basis for this differential stability, of male and female centrosomes in the same cytoplasm, is a mystery.

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