Antioxidants in IVF and culture media significantly improve human embryo development: A prospective randomised multi-centre trial

2018 ◽  
Author(s):  
Atsumi Yoshida
Keyword(s):  
Embryo Development ◽  
Human Embryo ◽  
Culture Media ◽  
Human Embryo Development

Improvement in early human embryo development using new formulation sequential stage-specific culture media

2002 ◽  
Vol 78 (6) ◽  
pp. 1254-1260 ◽  
Author(s):  
Simon Cooke ◽  
Patrick Quinn ◽  
Lee Kime ◽  
Cheryl Ayres ◽  
John P.P Tyler ◽  
...  
Keyword(s):  
Embryo Development ◽  
Human Embryo ◽  
Culture Media ◽  
Human Embryo Development ◽  
Specific Culture ◽  
New Formulation ◽  
Early Human

scholarly journals Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

2012 ◽  
Vol 29 (9) ◽  
pp. 891-900 ◽  
Author(s):  
Haydar Nadir Ciray ◽  
Turan Aksoy ◽  
Cihan Goktas ◽  
Bilgen Ozturk ◽  
Mustafa Bahceci
Keyword(s):  
Embryo Development ◽  
Human Embryo ◽  
Culture Media ◽  
Time Lapse ◽  
Human Embryo Development ◽  
Single Versus

scholarly journals MiR-191-5p Is Upregulated in Culture Media of Implanted Human Embryo on Day Three of Development

2020 ◽  
Author(s):  
Ricardo Josue Acuña-González ◽  
Fela Vanesa Morales-Hernández ◽  
Jorge Skiold López-Canales ◽  
Jair Lozano-Cuenca ◽  
Mauricio Osorio-Caballero ◽  
...  
Keyword(s):  
Embryo Development ◽  
Human Embryo ◽  
Culture Medium ◽  
Culture Media ◽  
Expression Profiles ◽  
Uterine Cavity ◽  
Common Criteria ◽  
Significant Difference ◽  
Human Embryo Development

Abstract Background: Morphologic features are the most common criteria for selecting human embryo to be transferred to the receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. The aim of this study was to quantify the expression profile of hsa-miR-21-3p, -24-1-5p, -191-5p, and -372-5p on day 3 of culture media from in vitro fertilization (IVF) embryo that were implanted or failed to be implanted in patients (n=25 pregnant and 25, non-pregnant patients). Methods: Fifty patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. On day 3 of development, embryos were transferred to women, and the culture medium was collected from implanted embryos (n=25, pregnant patients) and non-implanted embryos (n=25, non-pregnant). In the culture medium, RNA was isolated using TRIzol reagent. MiRNA expression was detected through RT-PCR with specific primers. Expression bands were quantified using an optic density.Results: The expression profiles were compared between pregnant and non-pregnant patients revealing a significant 5.2-fold greater expression of hsa-miR-191-5p in the former group (p ≤0.001) and a significantly higher expression of hsa-miR-24-1-5p (p =0.043) in the latter. No significant difference was found between the two groups in regard hsa-miR-21-3p or hsa-miR-372-5p (p =0.41). Conclusions: According to the results, has-miR-191-5p could possibly be a possible biomarker of adequate human embryo development. This miRNA modulated IGF2BP-1 and IGF2R, which are associated with the implantation window. On the other hand, hsa-miR-24-1-5p may be related to a poor prognosis of human embryo development.

scholarly journals Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Maria Vera-Rodriguez ◽  
Shawn L. Chavez ◽  
Carmen Rubio ◽  
Renee A. Reijo Pera ◽  
Carlos Simon
Keyword(s):  
Prediction Model ◽  
Single Cell ◽  
Embryo Development ◽  
Human Embryo ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Human Embryo Development ◽  
Early Human

scholarly journals The effect of modification of the embryo culture environment on human embryo development

2018 ◽  
Vol 110 (4) ◽  
pp. e365-e366
Author(s):  
Y. Kida ◽  
S. Yamada ◽  
N. Kawakita ◽  
T. Yoshimura ◽  
N. Fukunaga ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Human Embryo ◽  
Human Embryo Development ◽  
Culture Environment

Fertilization and early embryology: CD44 is expressed throughout pre-implantation human embryo development

1995 ◽  
Vol 10 (2) ◽  
pp. 425-430 ◽  
Author(s):  
S. Campbell ◽  
H.R. Swann ◽  
J.D. Aplin ◽  
M.W. Seif ◽  
S.J. Kimber ◽  
...  
Keyword(s):  
Embryo Development ◽  
Human Embryo ◽  
Human Embryo Development
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