The Sinorhizobium fredii HH103 type III secretion system effector NopC blocks nodulation with Lotus japonicus Gifu

2020 ◽  
Author(s):  
Irene Jiménez-Guerrero ◽  
Jose-Maria Vinardell-González ◽  
Francisco Pérez Montaño ◽  
Carlos Medina
Keyword(s):  
Type Iii Secretion ◽  
Lotus Japonicus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Sinorhizobium Fredii ◽  
Type Iii

scholarly journals The Sinorhizobium fredii HH103 type III secretion system effector NopC blocks nodulation with Lotus japonicus Gifu

2020 ◽  
Vol 71 (19) ◽  
pp. 6043-6056 ◽  
Author(s):  
Irene Jiménez-Guerrero ◽  
Sebastián Acosta-Jurado ◽  
Carlos Medina ◽  
Francisco Javier Ollero ◽  
Cynthia Alias-Villegas ◽  
...  
Keyword(s):  
Host Range ◽  
Type Iii Secretion ◽  
Lotus Japonicus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Infection Thread ◽  
Sinorhizobium Fredii ◽  
Model Legume ◽  
Type Iii ◽  
Infection Threads

Abstract The broad-host-range bacterium Sinorhizobium fredii HH103 cannot nodulate the model legume Lotus japonicus Gifu. This bacterium possesses a type III secretion system (T3SS), a specialized secretion apparatus used to deliver effector proteins (T3Es) into the host cell cytosol to alter host signaling and/or suppress host defence responses to promote infection. However, some of these T3Es are recognized by specific plant receptors and hence trigger a strong defence response to block infection. In rhizobia, T3Es are involved in nodulation efficiency and host-range determination, and in some cases directly activate host symbiosis signalling in a Nod factor-independent manner. In this work, we show that HH103 RifR T3SS mutants, unable to secrete T3Es, gain nodulation with L. japonicus Gifu through infection threads, suggesting that plant recognition of a T3E could block the infection process. To identify the T3E involved, we performed nodulation assays with a collection of mutants that affect secretion of each T3E identified in HH103 RifR so far. The nopC mutant could infect L. japonicus Gifu by infection thread invasion and switch the infection mechanism in Lotus burttii from intercellular infection to infection thread formation. Lotus japonicus gene expression analysis indicated that the infection-blocking event occurs at early stages of the symbiosis.

scholarly journals Translocation of NopP by Sinorhizobium fredii USDA257 into Vigna unguiculata Root Nodules

2010 ◽  
Vol 76 (11) ◽  
pp. 3758-3761 ◽  
Author(s):  
Lisa M. Schechter ◽  
Jeanette Guenther ◽  
Elizabeth A. Olcay ◽  
Sungchan Jang ◽  
Hari B. Krishnan
Keyword(s):  
Vigna Unguiculata ◽  
Type Iii Secretion ◽  
Root Nodules ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Dependent Manner ◽  
Nitrogen Fixing ◽  
Sinorhizobium Fredii ◽  
Type Iii

ABSTRACT Sinorhizobium fredii is a nitrogen-fixing legume symbiont that stimulates the formation of root nodules. S. fredii nodulation of roots is influenced by Nop proteins, which are secreted through a type III secretion system (T3SS). We demonstrate that S. fredii injects NopP into Vigna unguiculata nodules in a T3SS-dependent manner.

Type III Secretion System–Associated Toxins, Proteases, Serotypes, and Antibiotic Resistance ofPseudomonas aeruginosaIsolates Associated with Keratitis

2006 ◽  
Vol 31 (4) ◽  
pp. 297-306 ◽  
Author(s):  
Hua Zhu ◽  
Tim C.R. Conibear ◽  
Rani Bandara ◽  
Yulina Aliwarga ◽  
Fiona Stapleton ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii
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