Melphan As Anticancer And New Insights Of Drug As Parenteral Dosage Form Against SARS-CoV-2 By Using Development And Validation

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 1437-1446
Author(s):  
Vijayalakshmi Kancharla ◽  
Kumar Abbaraju V D N ◽  
Andrews B S A ◽  
Nagaraju Dasari ◽  
Nagu Korupolu
Keyword(s):  
Hplc Method ◽  
High Dose ◽  
Isopropyl Ester ◽  
Stressed Condition ◽  
Chromatography Analysis ◽  
Induction Regimen ◽  
Uv Visible ◽  
Development And Validation ◽  
Better Than

A validated HPLC method was developed for the determination of Melphan (MPA) in pharmaceutical formulation. LC 20AT pump and UV-Visible detector with flexible wavelength programme and Rheodyne injector are used in this present problem. Phenomenex Synergi C18, 250 mm × 4.6 mm, 4µm or equivalent is used for this chromatography analysis. Column used in this measurement is Phenomenex Synergi C18,250mm×4.6mm,4µm or equivalent. Specificity (stressed condition) is confirmed by no co-elution of diluent peaks, Blank as diluent, excipients peaks, Monohydroxy MPA, Dihydroxy MPA, Isopropyl ester and dimer impurity were not interfere with MPA Peak along with each other. Obtained results are 1.2 and 270103 respectively. The method developed is simple and is better than the methods reported in the literature. With the help of different studies, therefore, we recommend pursuing an induction regimen of up to six cycles in all patients to postpone treatment with high-dose MPA. By using various studies, we recommend pursuing an induction regimen of up to six cycles in all patients to postpone treatment with high-dose MPA for SARS-CoV-2.

scholarly journals Development and Validation of RP-HPLC Method for Estimation of Amlodipine Besylate and Celecoxib in Pharmaceutical Formulation

2020 ◽  
Vol 10 (6) ◽  
pp. 31-36
Author(s):  
P Nagamani ◽  
SY Manjunath ◽  
T Hemant Kumar
Keyword(s):  
High Performance ◽  
Pharmaceutical Products ◽  
Hplc Method ◽  
Amlodipine Besylate ◽  
Linear Calibration ◽  
Rp Hplc ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage

A simple, precise, accurate, and rapid reverse phase-high performance liquid chromatography (RP-HPLC) method with UV-Visible detector has been developed and subsequently validated for the simultaneous determination of amlodipine besylate(AML) and celecoxib(CEL) in their combined tablet dosage form. The separation was based on the use of a Flowrosil C18   analytical column (250 × 4.6 mm, i.d., 5 µm). The mobile phase consisted of a mixture of 80 volumes of acetonitrile and 20 volumes of water. The chromatography was performed by isocratic elution at a flow rate of 1 mL/min. Analytes were detected at 250 nm, with linear calibration curves at concentration ranges of 2-12 µg/ml and 50-300 µg/ml for AML and CEL respectively. The retention time of AML and CEL were 1.98 and 3.15 min respectively. The recoveries obtained were 99.46‒101.36% for AML, 99.57‒101.42% and 99.96–100.87 % for CEL. The method was validated according to International conference of harmonisation guidelines in terms of accuracy, precision, specificity, robustness, limits of detection and quantitation, and other aspects of analytical validation. The developed method was applied successfully for HPLC analysis of commercial pharmaceutical products including AML and CEL. Keywords: Amlodipine besylate; Celecoxib; RP-HPLC.

scholarly journals Development and Validation of Rapid and Sensitive HPLC Method for the Determination of Methotrexate in Human Serum

2010 ◽  
Vol 2 (1) ◽  
pp. 8-13 ◽  
Author(s):  
M Nagulu ◽  
V Uday Kiran ◽  
Y Narsimha Reddy ◽  
D Rama Krishna
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Hplc Method ◽  
High Dose ◽  
Chromatography Method ◽  
Quantitation Limit ◽  
High Doses ◽  
Liquid Chromatography Method ◽  
Development And Validation

Methotrexate competitively inhibits dihydrofolic acid reductase and thereby inhibits DNA synthesis and cellular replication.This study describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate [MTX] in serum.samples were collected from adult cancer patients receiving high dose MTX at Mahathma Gandhi Memorial hospital (Warangal,AP.India) at various time intervals after the end of each infusion. Serum was deproteinized with trichloroacetic acid and the supernatant was injected into a 250×4.6 mm octadecylsilane column. Mobile phase was made of TRIS-phosphate buffer (pH 5.7): methanol: acetonitrile (70:20:10) with a flow rate of 1ml/min. Ultraviolet detection was done at 313 nm and at ambient temperature. Para aminoacetophenone was used as internal standard. Methotrexate and internal standard retention times were 4.6 and 9.5 minutes, respectively. Results showed that reproducibility (precision) of method within a day was 2.6 to 6 percent and between days was 5.5 to 9.5 percent. The recovery of the method was between 61.5 and 72.7 percent. The quantitation limit of the method for methotrexate was 0.1μM. This method is suitable for quantitation of methotrexate after infusion of high doses of this drug and has good accuracy, precision and quantitation limit. Key Words: Methotrexate; HPLC; Serum Concentration. DOI: 10.3329/sjps.v2i1.1693Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 8-13

scholarly journals DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR DETERMINATION OF ERDAFITINIB IN MARKETED FORMULATIONS

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
M. Maithani ◽  
D. Dwivedi ◽  
D. Hatwal ◽  
P. Bansal
Keyword(s):  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Sodium Acetate Buffer ◽  
Rp Hplc ◽  
The Mean ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage

A simple and precise RP-HPLC method for the estimation of Erdafitinib in tablet dosage form was developed and validated. The chromatographic separation of the drug was done with a Hypersil™ ODS C18 Column (150 mm × 4.6 mm i.d., particle size 5 μ) using 20mM sodium acetate buffer (pH 4. ±0.02), methanol and acetonitrile (60:10:30 v/v/v) as a mobile phase. The instrument was set at flow rate of 1.0 mLmin-1 at ambient temperature and the wavelength of UV-visible detector at 310nm. The method showed excellent linearity over a range of 5-35 μgmL-1 for the drug. The correlation coefficient for Erdafitinib was noted to be 0.9999. The mean recovery values were found to be 99.77% and 100.88%. The results suggest that the proposed method could be suitable for quantitative determination of Erdafitinib in pharmaceutical preparations and also for quality control in bulk manufacturing. The F-test and t-test at 95% confidence level were applied on data for statistical analysis.

QUANTITATIVE DETERMINATION OF CURCUMIN IN TURMERIC POWDER AND TURMERIC–HONEY BALL PILLS BY HPLC

2018 ◽  
Vol 8 (4) ◽  
pp. 42-47
Author(s):  
Tien Nguyen Huu ◽  
Tram Le Thi Bao ◽  
Ngoc Nguyen Thi Nhu ◽  
Thang Phan Phuoc ◽  
Khan Nguyen Viet
Keyword(s):  
Acetic Acid ◽  
Gastric Mucosa ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Curcuma Longa ◽  
Biological Marker ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Precision And Accuracy

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC

Development and Validation of a Rapid UV-HPLC Method for the Determination of Anidulafungin in Perfusion Solution

2017 ◽  
Vol 13 (3) ◽  
pp. 314-320
Author(s):  
Gabriel Estan-Cerezo ◽  
Francisco Jose Rodriguez-Lucena ◽  
Ana Cristina Murcia-López ◽  
Carmen Matoses Chirivella ◽  
Vanesa Escudero-Ortiz ◽  
...  
Keyword(s):  
Hplc Method ◽  
Perfusion Solution ◽  
Development And Validation
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