scholarly journals Fast and Accurate Quantitative Determination of Prednisolone and Chlorpheniramine in Active Pharmaceutical Ingredients and Parenteral Dosage Forms

2021 ◽  
Vol 5 (11) ◽  
pp. 1922-1926
Keyword(s):  
Quantitative Determination ◽  
Dosage Forms ◽  
Active Pharmaceutical Ingredients ◽  
Pharmaceutical Ingredients

scholarly journals DEVELOPMENT AND VALIDATION OF METHODS FOR QUANTITATIVE DETERMINATION OF ACTIVE PHARMACEUTICAL SUBSTANCES IN NASAL SPRAY

2021 ◽  
Vol 9 (4) ◽  
pp. 266-277
Author(s):  
M. V. Larskiy ◽  
A. E. Pozdnyakova ◽  
Z. D. Khadzhieva ◽  
D. I. Pozdnyakov
Keyword(s):  
Quantitative Determination ◽  
Nasal Spray ◽  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
Dihydrogen Phosphate ◽  
Active Pharmaceutical Ingredients ◽  
Potassium Dihydrogen ◽  
Pharmaceutical Ingredients ◽  
Development And Validation

Intranasal administration of H1-histamine receptor blockers may be a promising approach to the treatment of allergic rhinitis. Earlier, an original composition of a nasal spray containing fexofenadine hydrochloride and ammonium glycyrrhizinate and demonstrating a high level of therapeutic efficacy, was developed.The aim of the study was to develop and validate a method of the quantitative determination of active pharmaceutical ingredients fexofenadine hydrochloride and ammonium glycyrrhizinate in a spray for intranasal administration.Materials and methods. During the development and validation of the method of the fexofenadine hydrochloride and ammonium glycyrrhizinate quantitative determination in a nasal spray, the method of high performance liquid chromatography was used: a Dionex Ultimate 3000 UV chromatograph with a Luna C18 column (2) containing octadecylsilicagel with a 5 μm grain size as a sorbent. The analysis and validation procedures were performed in accordance with the requirements of the State Pharmacopoeia of the Russian Federation, the XIVth edition.Results. The study showed that for the simultaneous quantitative determination of fexofenadine hydrochloride and ammonium glycyrrhizinate, the optimal elution regime is a gradient mode with a mobile phase containing 50 mmol/L potassium dihydrogen phosphate solution with methanol (45:55), which ensured the separation of the components in the 20 minutes interval. The validation procedures showed that the developed methodology correspond to all the criteria of validity in terms of the following indicators: correctness, precision, specificity and linearity in the analytical area.Conclusion. The obtained results indicate the possibility of using the method of high-performance liquid chromatography in a gradient elution mode with a mobile phase of the composition of a 50 mmol/L solution of potassium dihydrogen phosphate with methanol (45:55) for the simultaneous quantitative determination of active pharmaceutical ingredients – fexofenadine hydrochloride and ammonium glycyrrhizinate as parts of a promising nasal spray for the allergic rhinitis treatment.

Simultaneous Determination of Emtricitabine and Tenofovir disoproxil fumarate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography

2021 ◽  
pp. 412-416
Author(s):  
Rajan V. Rele ◽  
Sandip P. Patil
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Tenofovir Disoproxil Fumarate ◽  
High Performance ◽  
Phosphoric Acid Solution ◽  
Active Pharmaceutical Ingredients ◽  
Chromatography Method ◽  
Pharmaceutical Ingredients

A simple, rapid and accurate high performance liquid chromatography method is described for simultaneous determination of emtricitabine and tenofovir disoproxil fumarate from active pharmaceutical ingredients. The separation of drug was achieved on Hypersil BDS C18 (150 x 4.6 mm i.d.) with 5 µ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and methanol (85:15 % v/v). The buffer was mixtures of 0.1 % (v/v) ortho-phosphoric acid solution adjusted the pH 3.5 with tri-ethyl amine. The detection was carried out at wavelength 260 nm. The mixture of buffer of pH 3.5 and methanol (85:15% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze emtricitabine and tenofovir disoproxil fumarate from active pharmaceutical ingredients.

GLC Determination of the Optical Isomers of Amphetamine

1970 ◽  
Vol 53 (1) ◽  
pp. 113-115
Author(s):  
Clyde E Wells
Keyword(s):  
Quantitative Determination ◽  
Calibration Curve ◽  
Collaborative Study ◽  
Isomeric Ratio ◽  
Dosage Forms ◽  
Optical Isomers ◽  
Standard Calibration ◽  
Tablet Dosage

Abstract A method is described for the quantitative determination of the ratio of d- and l-amphetamine stereoisomers by GLC. A derivative formed with N-trifluoroacetyl-(l)-prolyl chloride is chromatographed and the isomeric ratio is read from a standard calibration curve. The method is applicable to crystalline salts of amphetamine and to commercial tablet dosage forms. It is recommended that this method be subjected to collaborative study.

scholarly journals Spectrophotometric Determination of Ritodrine and Isoxsuprine Hydrochlorides Using 4-Aminoantipyrine

2000 ◽  
Vol 83 (6) ◽  
pp. 1440-1445 ◽  
Author(s):  
Hosakere D Revanasiddappa ◽  
Bochhe Gowda Manju
Keyword(s):  
Quantitative Determination ◽  
Rapid Method ◽  
Spectrophotometric Determination ◽  
Dosage Forms ◽  
Oxidizing Agent ◽  
Ritodrine Hydrochloride ◽  
Colored Product

Abstract A simple, accurate, and rapid method for the quantitative determination of ritodrine hydrochloride (RTH) and isoxsuprine hydrochloride (ISH) in both pure and dosage forms, is described. The method is based on the development of pink colored product as a result of the condensation of 4-aminoantipyrine with phenols in the presence of an alkaline oxidizing agent. The resulting products are measured at 510 nm for both drugs, with molar absorptivities of 0.98 × 104 and 1.20 × 104 L/mol·cm for RTH and ISH, respectively. A study of the effect of commonly associated excipients revealed that they did not cause interference.

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