scholarly journals Polarization-interference mapping of networks in diffusal polycristaline biological tissues

Objectives: Development and experimental testing of the complex of Stokes-polarimetry and interferometry methods using algorithms for digital holographic reconstruction of the amplitude-phase structure of object fields for obtaining interrelationships between 3D distributions of depolarization maps and peculiarities of the polycrystalline structure of histological sections of biological tissues of different morphological structures and physiological state. Materials and methods: The basis of the 3D Müller-matrix mapping method is the use of a reference wave of laser radiation, which is superimposed on a polarization-non-uniform image of the biological layer in the scheme of the optical interferometer. Results: In the process of comparative analysis of the map of depolarization of biological tissues with different geometric scales of the morphological structure, we found different rates of growth of the degree of depolarization.

2019 ◽  
Vol 35 (4) ◽  
pp. 877-891 ◽  
Author(s):  
L. Trifonyuk ◽  
A. Sdobnov ◽  
W. Baranowski ◽  
V. Ushenko ◽  
O. Olar ◽  
...  

AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility of experimental data. This is due to azimuthal dependence of polarization and ellipticity values of most matrix elements to sample orientation in respect to incidence light polarization. Current study aims to generalize the methods of laser polarimetry for diagnosis of partially depolarizing optically anisotropic biological tissues. A method of differential Mueller matrix mapping for reconstruction of linear and circular birefringence and dichroism parameter distributions of partially depolarizing layers of biological tissues of different morphological structure is introduced and practically implemented. The coordinate distributions of the value of the first-order differential matrix elements of histological sections of brain tissue with spatially structured, optically anisotropic fibrillar network, as well as of parenchymatous tissue of the rectum wall with an “islet” polycrystalline structure are determined. Within the statistical analysis of polarization reproduced distributions of the averaged parameters of phase and amplitude anisotropy, the significant sensitivity of the statistical moments of the third and fourth orders to changes in the polycrystalline structure of partially depolarizing layers of biological tissue is observed. The differentiation of female reproductive sphere connective tissue is realized with excellent accuracy. The differential Mueller matrix mapping method for reconstruction of distributions of linear and circular birefringence and dichroism parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. Differential diagnostics of changes in the phase (good balanced accuracy) and amplitude (excellent balanced accuracy) of the anisotropy of the partially depolarizing layers of the vagina wall tissue with prolapse of the genitals is realized. The maximum diagnostic efficiency of the first-order differential matrix method was demonstrated in comparison with the traditional methods of polarization and Mueller matrix mapping of histological sections of light-scattering biological tissues.


2019 ◽  
Vol 35 (1) ◽  
pp. 36-44
Author(s):  
O.V. Dubolazov ◽  
◽  
M.Yu. Sakhnovsky ◽  
O.V. Olar ◽  
P.M. Grigorishin ◽  
...  

2021 ◽  
Author(s):  
N.V. Kovalenko ◽  
K.V. Sovin ◽  
O.A. Ryabushkin

Problem formulating. The vital processes of biological tissues are closely related to their electrical properties. An important task is to create a physical and mathematical model that will link the electrical properties of tissues to their physiological state. Goal. Construction of a model of biological tissue electrical properties based on the equations of ion electrodiffusion. Result. The paper presents the model of biological tissue electrical properties based on the ion electrodiffusion equations, and compares the simulation results with the experimental results presented in the literature. Practical meaning. The presented model can be used to describe processes occurring in tissue at the level of concentration and conductivity of ions in individual cells and cell membranes. In particular, the process of tissue degradation during laser radiation heating can be described.


2000 ◽  
Author(s):  
Ray W. Ogden ◽  
Christian A. J. Schulze-Bauer

Abstract In this paper we present some new data from extension-inflation tests on a human iliac artery and then, on the basis of the nonlinear theory of elasticity, we examine a possible model to represent this data. The model considers the artery initially as a thick-walled circular cylindrical tube which may consist of two or more concentric layers. In order to take some account of the architecture (morphological structure), each layer of the material is regarded as consisting of two families of mechanically equivalent helical fibers symmetrically disposed with respect to the cylinder axis. The resulting material properties are then orthotropic in each layer. General formulas for the pressure and the axial load in the symmetric inflation of an extended tube are obtained. The starting point is the unloaded circular cylindrical configuration, but (in general unknown) residual stresses are included in the formulation. The model is illustrated by specializing firstly to the case of a single layer so that the consequences of the hypothesis of uniform circumferential stress in the physiological state can be examined theoretically. This enables the required residual stresses to be calculated explicitly. Secondly, the equations are specialized for the membrane approximation in order to show how certain important characteristics of the experimental data can be replicated using a relatively simple anisotropic membrane model.


2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Oleg V. Angelsky ◽  
Yuriy A. Ushenko

To characterize the degree of consistency of parameters of the optically uniaxial birefringent liquid crystals (protein fibrils) nets of biological tissues a new parameter-complex degree of mutual anisotropy is suggested. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues is developed. It is shown that statistic approach to the analysis of complex degree of mutual anisotropy distributions of biological tissues of various morphological and physiological states and optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state in comparison with investigations of complex degree of mutual polarization of the corresponding laser images.


2019 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Alexander Ushenko ◽  
Anton Sdobnov ◽  
Alexander Dubolazov ◽  
Marta Grytsiuk ◽  
Yuriy Ushenko ◽  
...  

2015 ◽  
Vol 29 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Ákos Kertész ◽  
Zuzana Hlaváčová ◽  
Eszter Vozáry ◽  
Lenka Staroňová

Abstract Electrical properties of food materials can give information about the inner structure and physiological state of biological tissues. Generally, the process of drying of fruits and vegetables is followed by weight loss. The aim of this study was to measure the impedance spectra of carrot slices during drying and to correlate impedance parameters to moisture content in different drying periods. Cylindrical slices were cut out from the carrot root along the axis. The slices were dried in a Venticell 111 air oven at 50°C. The weight of the slices was measured with a Denver SI-603 electronic analytical and precision balance. The weighing of the samples was performed every 30 min at the beginning of drying and every 60 min after the process. The moisture content of the samples was calculated on wet basis. The magnitude and phase angle of electrical impedance of the slices were measured with HP 4284A and 4285A precision LCR meters in the frequency range from 30 Hz to 1 MHz and from 75 kHz to 30 MHz, respectively, at voltage 1 V. The impedance measurement was performed after weighting. The change in the magnitude of impedance during drying showed a good correlation with the change in the moisture content.


Sign in / Sign up

Export Citation Format

Share Document