Rhodnius pallescens microsatellite markers for population genetic analysis in Rhodnius ecuadoriensis: preliminary assessment

Rhodnius ecuadoriensis Lent & León (Hemiptera: Reduviidae) es el prinicipal vector de la enfermedad de Chagas en Ecuador, donde la estructura genética de sus poblaciones es poco conocida. Nosotros probamos seis Repeticiones Cortas en Tamdem (RCT) de R. pallescens Barber en poblaciones selváticas y domésticas de R. ecuadoriensis. Dos microsatelites fueron monomórficos, dos dieron resultados ambiguos y dos fueron polimórficos (16 y 19 alelos) y fueron utilizados para análisis. Los resultados de las frecuencias alélicas, AMOVA y los pruebas Bayesianas para genética favorecen la teoría de la existencia de una sola población. Estos resultados preliminares sugieren que las poblaciones selváticas y domésticas d R. ecuadoriensis intercambian frecuentemente migrantes. Por consiguiente el control de la Enfermedad de Chagas requiere vigilancia entomológica continua en la costa del Ecuador.

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Rhodnius pallescens microsatellite markers for population genetic analysis in Rhodnius ecuadoriensis: preliminary assessment

Revista Ecuatoriana de Medicina y Ciencias Biológicas ◽

10.26807/remcb.v40i2.763 ◽

2019 ◽

Vol 40 (2) ◽

Author(s):

Mario J. Grijalva

Keyword(s):

Genetic Analysis ◽

Microsatellite Markers ◽

Population Genetic ◽

Preliminary Assessment ◽

Population Genetic Analysis ◽

Rhodnius Ecuadoriensis ◽

Rhodnius Pallescens

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Population Genetic Analysis among Five Indian Population Groups Using Six Microsatellite Markers

Human Biology ◽

10.1353/hub.2003.0032 ◽

2003 ◽

Vol 75 (2) ◽

pp. 189-203 ◽

Cited By ~ 1

Author(s):

Anu Ghosh ◽

Birajalaxmi Das ◽

M Seshadri

Keyword(s):

Genetic Analysis ◽

Microsatellite Markers ◽

Population Genetic ◽

Indian Population ◽

Population Genetic Analysis ◽

Population Groups

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The development of 10 novel polymorphic microsatellite markers through next generation sequencing and a preliminary population genetic analysis for the endangered Glenelg spiny crayfish, Euastacus bispinosus

Molecular Biology Reports ◽

10.1007/s11033-013-2531-5 ◽

2013 ◽

Vol 40 (7) ◽

pp. 4415-4419 ◽

Cited By ~ 10

Author(s):

Adam D. Miller ◽

Anthony Van Rooyen ◽

Oisín F. Sweeney ◽

Nick S. Whiterod ◽

Andrew R. Weeks

Keyword(s):

Next Generation Sequencing ◽

Genetic Analysis ◽

Microsatellite Markers ◽

Population Genetic ◽

Next Generation ◽

Population Genetic Analysis ◽

Polymorphic Microsatellite ◽

Generation Sequencing

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Population genetic analysis and conservation strategies for redtail shrimp Fenneropenaeus penicillatus using ten microsatellite markers

Genetics and Molecular Research ◽

10.4238/gmr16019068 ◽

2017 ◽

Vol 16 (1) ◽

Cited By ~ 2

Author(s):

Y.Y. Cao ◽

J.B. Shangguan ◽

Z.B. Li

Keyword(s):

Genetic Analysis ◽

Microsatellite Markers ◽

Population Genetic ◽

Conservation Strategies ◽

Population Genetic Analysis

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Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210921 ◽

2020 ◽

Vol 21 (9) ◽

Author(s):

Yash Munnalal Gupta ◽

SUPATCHAREE TANASARNPAIBOON ◽

KITTISAK BUDDHACHAT ◽

SURIN PEYACHOKNAGUL ◽

PHATTHARAPORN INTHIM ◽

...

Keyword(s):

Genetic Variation ◽

Genetic Analysis ◽

Microsatellite Markers ◽

Population Genetic ◽

Acheta Domesticus ◽

House Cricket ◽

Population Genetic Analysis ◽

Closely Related Species ◽

Genetic Studies ◽

Future Population

Abstract. Gupta YM, Tanasarnpaiboon S, Buddhachat K, Peyachoknagul S, Inthim P, Homchan S. 2020. Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Biodiversitas 21: 4094-4099. The house cricket, Acheta domesticus, is one of the species of crickets commonly found in Thailand. Insect breeders in Thailand prefer to breed house cricket as food due to its better taste and popularity among local people. Moreover, largescale breeding industries also breed house cricket to produce cricket-based edible products. Insect breeding industry is growing rapidly and requires primary precaution for sustainable production. To facilitate breeding system to maintain genetic variation in the captive population, we have sequenced the whole genome of A. domesticus to search for simple sequence repeats (SSRs) in order to develop polymorphic microsatellite markers for preliminary population genetic analysis. A total of 112,157 SSRs with primer pairs were identified in our analysis. Of these, 91 were randomly selected to check for amplification of microsatellite polymorphisms. From these, nine microsatellites were used to check genetic variation in forty-five individuals of A. domesticus from the Phitsanulok population (Thailand). These microsatellite markers also showed cross-amplification with other three species of edible crickets, specifically Gryllus bimaculatus, Gryllus testaceus, and Brachytrupes portentosus. The microsatellite markers presented herein will facilitate future population genetic analysis of A. domesticus populations. Moreover, the transferability of these makers would also enable researchers to conduct genetic studies for other closely related species.

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