First isolation of methicillin-resistant Staphylococcus aureus from pigs’ clinical samples in Serbia

Methicillin-resistant Staphylococcus aureus is a highly important human pathogen that is also a significant concern in veterinary medicine. Despite the high prevalence of colonization, clinical infections with methicillin-resistant Staphylococcus aureus appear to be rare in pigs. Methicillin-resistant Staphylococcus aureus was isolated from a sow with endometritis and her five piglets with dermatitis originating from a Serbian farm. Identification of the strains was done by automated system and confirmed by polymerase chain reaction for mecA and nuc genes. Detection of Staphylococcal Cassette Chromosome mec type was performed by multiplex polymerase chain reaction. Antimicrobial susceptibility testing on erythromycin, clindamycin, gentamicin, kanamycin, tobramycin, ciprofloxacin, tetracycline, trimethoprim/sulfamethoxazole and vancomycin was done by disc diffusion method. Six isolated strains from the infected sow and her piglets showed resistance only to tetracycline beside resistance to all beta-lactam antibiotics. In the tested methicillin-resistant Staphylococcus aureus isolates, Staphylococcal Cassette Chromosome mec type V was present. To our knowledge, this finding is the first documented detection of methicillin-resistant Staphylococcus aureus from pigs’ clinical samples in Serbia. The results of our study indicate the emergence of methicillin-resistant Staphylococcus aureus in a pig farm in Serbia highlighting the threat of this antibiotic-resistant microorganism as a pathogen causing both animal and human infections.

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IDENTIFICATION OF SCCMEC TYPE IN ISOLATE OF METHICILLIN RESISTANT Staphylococcus aureus (MRSA) IN JAMBI BY USING POLYMERASE CHAIN REACTION (PCR)

Indonesian Journal of Tropical and Infectious Disease ◽

10.20473/ijtid.v8i2.12691 ◽

2020 ◽

Vol 8 (2) ◽

pp. 77

Author(s):

Humaryanto Humaryanto ◽

Hanina Hanina ◽

Lipinwati Lipinwati ◽

Charles Apul Simanjuntak

Keyword(s):

Staphylococcus Aureus ◽

Polymerase Chain Reaction ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sccmec Type ◽

Diffusion Method ◽

Clinical Samples ◽

Chain Reaction ◽

Methicillin Resistant ◽

Type Iii ◽

Polymerase Chain

Staphylococcal cassette chromosome mec (SCCmec) is one of the mobile genetic elements of Methicillin-Resistant Staphylococcus aureus (MRSA) that carries many resistance genes and allows SCCmec to move from one bacterium to another. Twelve types of SCCmec have been identified throughout the world. Identification of SCCmec type is needed to determine the pattern of MRSA resistance in a particular region. This study aimed to identify the type of SCCmec MRSA from clinical samples. Specifically, this study was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences of Jambi University in June 2018-February 2019. Culture was carried out on 100 clinical specimens of festering wound swabs from inpatients at hopitals in Jambi City. A total of 32 samples of Staphytect plus test positive were tested using Cefoxitin disc diffusion method and MecA Polymerase Chain Reaction (PCR). There were 14 samples identified as MRSA isolates, namely twelve samples (85.72%) of SCCmec type III, one sample (7.14%) of SCCmec type II, and one sample (7.14%) of SCCmec type IVb. The results were different from previous studies where all MRSA isolates (100%) in Indonesia were SCCmec type III, although most SCCmec types were still dominated by SCCmec type III. This study concludes that there has been a shift in the content of SCCmec in MRSA isolate originating from hospitals in Jambi city.

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