scholarly journals Isolation of Endophytic Fungi from Douglas-Fir (Pseudotsuga menziesii) Foliage with Bioprospecting Potential for Natural Pharmaceuticals

2019 ◽  
Vol 3 (01) ◽  
1966 ◽  
Vol 44 (4) ◽  
pp. 413-420 ◽  
Author(s):  
W. J. Bloomberg

Segments taken from the roots and shoots of healthy 1-year-old seedlings of Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) collected over a 2-year period from two British Columbia forest nurseries, were surface-sterilized, and incubated on various media. Fungi grew from segments of over 80% of the seedlings, the most frequent being Fusarium oxysporum Schlecht., F. redolens Wr., Mycelium radicis atrovirens, Cylindrocarpon didymum (Hart.) Wollenw., and C. radicicola Wollenw. Fungi were isolated more frequently from the shoot than from the root segments. Isolations from segments with the bark intact were more frequent than from segments that had the bark removed. Seedlings collected in the winter produced more fungi than those collected during the summer. Segments from diseased seedlings and seedlings grown under sterile conditions produced mycofloras which were different from each other and from that of healthy nursery-grown seedlings. Without exception, fungi grew from surface-sterilized seed placed on malt agar and samples from different seedlots had different floras. Stained sections clearly showed hyphae and chlamydospores well within the tissue of roots, shoots, and seed coat.


Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 744-748 ◽  
Author(s):  
Jerry E. Weiland ◽  
Bryan R. Beck ◽  
Anne Davis

Pythium species are common soilborne oomycetes that occur in forest nursery soils throughout the United States. Numerous species have been described from nursery soils. However, with the exception of P. aphanidermatum, P. irregulare, P. sylvaticum, and P. ultimum, little is known about the potential for other Pythium species found in nursery soils to cause damping-off of tree seedlings. A greenhouse study was conducted to evaluate the pathogenicity and virulence of 44 Pythium isolates representing 16 species that were originally recovered from soil at three forest nurseries in Washington and Oregon. Seeds of Douglas-fir (Pseudotsuga menziesii) were planted into soil infested with each of the isolates. Seedling survival, the number of surviving seedlings with necrotic root lesions, and taproot length were evaluated 4 weeks later. Responses of Douglas-fir to inoculation varied significantly depending on Pythium species and isolate. Eight species (P. dissotocum, P. irregulare, P. aff. macrosporum, P. mamillatum, P. aff. oopapillum, P. rostratifingens, P. sylvaticum, and P. ultimum var. ultimum) significantly reduced the number of surviving seedlings compared to the noninoculated treatment. However, all Pythium species caused a greater percentage of seedlings to develop root lesions (total mean 40%) than was observed from noninoculated seedlings (17%). Taproot length varied little among Pythium treatments and was not a useful character for evaluating pathogenicity. Results confirm the ability of P. irregulare, P. mamillatum, and P. ultimum var. ultimum to cause damping-off of Douglas-fir seedlings, and are indicative that other species such as P. dissotocum, P. aff. macrosporum, P. aff. oopapillum, P. rostratifingens, and P. sylvaticum may also be responsible for seedling loss.


1998 ◽  
Vol 28 (8) ◽  
pp. 1198-1206 ◽  
Author(s):  
Paige E Axelrood ◽  
William K Chapman ◽  
Keith A Seifert ◽  
David B Trotter ◽  
Gwen Shrimpton

Poor performance of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) plantations established in 1987 has occurred in southwestern British Columbia. Affected sites were planted with 1-year-old container stock that exhibited some root dieback in the nursery. A study was initiated in 1991 to assess Cylindrocarpon and Fusarium root infection in planted and naturally regenerating (natural) Douglas-fir seedlings from seven affected plantations. Percentages of seedlings harboring Cylindrocarpon spp.and percent root colonization were significantly greater for planted seedlings compared with natural seedlings. A significant linear trend in Cylindrocarpon root colonization was observed for planted seedlings with colonization levels being highest for roots closest to the remnants of the root plug and decreasing at distances greater than 10cm from that region. This trend in Cylindrocarpon colonization was not observed for natural seedlings. Cylindrocarpon destructans (Zins.) Scholten var. destructans and C.cylindroides Wollenw. var. cylindroides were the only species isolated from planted and natural conifer seedlings. For most sites, percentage of seedlings harboring Fusarium spp.and percent Fusarium root colonization were less than for Cylindrocarpon. Recovery of Fusarium spp.from seedlings and root colonization levels were not significantly different for planted and natural seedlings from all sites.


Author(s):  

Abstract A new distribution map is provided for Contarinia pseudotsugae Condrashoff (Diptera: Cecidomyiidae). Hosts: Douglas fir (Pseudotsuga menziesii). Information is given on the geographical distribution in Europe (Belgium, France, Germany and Netherlands) and North America (Canada, British Columbia, USA, California, Idaho, Michigan, Montana, Oregon, Pennsylvania and Washington).


Author(s):  

Abstract A new distribution map is provided for Phytophthora pseudotsugae Hamm & Hansen. Host: douglas fir (Pseudotsuga menziesii). Information is given on the geographical distribution in NORTH AMERICA, USA, OR, WA.


Author(s):  

Abstract A new distribution map is provided for Rhabdocline pseudotsugae Syd. Hosts: Douglas fir (Pseudotsuga menziesii). Information is given on the geographical distribution in EUROPE, Belgium, Britain, Czechoslovakia, Denmark, France, Germany, Irish Republic, Netherlands, Norway, Poland, Romania, Sweden, Switzerland, Yugoslavia, NORTH AMERICA, Canada (Alberta, British Columbia), USA.


Author(s):  

Abstract A new distribution map is provided for Dendroctonus pseudotsugae Hopkins Coleoptera: Scolytidae Hosts: Mainly Douglas fir (Pseudotsuga menziesii), also other Pseudotsuga spp. Information is given on the geographical distribution in NORTH AMERICA, Canada, Alberta, British Columbia, Mexico, USA, Arizona, California, Colorado, Idaho, Montana, Nevada, New Mexico, Oregon, Texas, Utah, Washington, Wyoming.


2019 ◽  
Vol 433 ◽  
pp. 105-110
Author(s):  
Matthew E. Hane ◽  
Andrew J. Kroll ◽  
Aaron Springford ◽  
Jack Giovanini ◽  
Mike Rochelle ◽  
...  

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