scholarly journals Model-guided design of mammalian genetic programs

2021 ◽  
Vol 7 (8) ◽  
pp. eabe9375
Author(s):  
J. J. Muldoon ◽  
V. Kandula ◽  
M. Hong ◽  
P. S. Donahue ◽  
J. D. Boucher ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Computational Models ◽  
Biological Complexity ◽  
Genetic Circuits ◽  
Cellular Functions ◽  
High Performing ◽  
Design Objective ◽  
Complex Functions ◽  
Mammalian Genetic ◽  
Analog Processing

Genetically engineering cells to perform customizable functions is an emerging frontier with numerous technological and translational applications. However, it remains challenging to systematically engineer mammalian cells to execute complex functions. To address this need, we developed a method enabling accurate genetic program design using high-performing genetic parts and predictive computational models. We built multifunctional proteins integrating both transcriptional and posttranslational control, validated models for describing these mechanisms, implemented digital and analog processing, and effectively linked genetic circuits with sensors for multi-input evaluations. The functional modularity and compositional versatility of these parts enable one to satisfy a given design objective via multiple synonymous programs. Our approach empowers bioengineers to predictively design mammalian cellular functions that perform as expected even at high levels of biological complexity.

Cultivating biological and cultural diversity: urban farming in Casamance, Senegal

Africa ◽  
1996 ◽  
Vol 66 (1) ◽  
pp. 104-121 ◽  
Author(s):  
Olga F. Linares
Keyword(s):  
Urban Agriculture ◽  
Primary Productivity ◽  
Urban Poor ◽  
West African ◽  
Lower Income ◽  
Biological Complexity ◽  
Urban Farming ◽  
Household Economies ◽  
City Environment ◽  
The City

AbstractAt the present time, urban agriculture—that is, the growing of food crops in backyard gardens, unused city spaces and peripheral zones—is an economically viable alternative for many African migrants. Although previously ‘invisible’ to most developers and economists, urban farming is now recognised as playing a crucial subsistence role in the household economies of lower-income people living in major West African cities. But the practice does more than feed the urban poor. Using the example of Ziguinchor in Casamance, Senegal, it is argued that growing crops in peri-urban and intra-urban zones, on otherwise neglected or half-built-up land, also protects and enriches the city environment while increasing the primary productivity of the inhabitants. Directly, or in more subtle ways, the practice strengthens bonds of friendship, and promotes inter-ethnic co-operation while at the same time helping to maintain biological complexity in interesting and previously unexplored ways. City farming may provide a context through which the urban poor can relate to debates about biodiversity.

scholarly journals Harnessing the biological complexity of Big Data from LINCS gene expression signatures

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0201937 ◽  
Author(s):  
Aliyu Musa ◽  
Shailesh Tripathi ◽  
Meenakshisundaram Kandhavelu ◽  
Matthias Dehmer ◽  
Frank Emmert-Streib
Keyword(s):  
Gene Expression ◽  
Big Data ◽  
Biological Complexity ◽  
Gene Expression Signatures

scholarly journals Synthetic protocell biology: from reproduction to computation

2007 ◽  
Vol 362 (1486) ◽  
pp. 1727-1739 ◽  
Author(s):  
Ricard V Solé ◽  
Andreea Munteanu ◽  
Carlos Rodriguez-Caso ◽  
Javier Macía
Keyword(s):  
Current Knowledge ◽  
Building Blocks ◽  
Biochemical Networks ◽  
Cellular Structures ◽  
Biological Complexity ◽  
Artificial Cell ◽  
Future Challenges ◽  
Cooperative Dynamics ◽  
The Origin Of Life ◽  
Review Current

Cells are the building blocks of biological complexity. They are complex systems sustained by the coordinated cooperative dynamics of several biochemical networks. Their replication, adaptation and computational features emerge as a consequence of appropriate molecular feedbacks that somehow define what life is. As the last decades have brought the transition from the description-driven biology to the synthesis-driven biology, one great challenge shared by both the fields of bioengineering and the origin of life is to find the appropriate conditions under which living cellular structures can effectively emerge and persist. Here, we review current knowledge (both theoretical and experimental) on possible scenarios of artificial cell design and their future challenges.

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