Positive experience of transplantation of a multicomponent composite containing allogeneic mesenchymal stem cells after resection of the rabbit bladder wall (description of one experiment).

There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marrow mesenchymal stem cells (BM-MSCs) were transfected into immortalized upregulated SDF-1-engineered BM-MSCs (imMSCs/eSDF-1+) or immortalized normal SDF-1-engineered BM-MSCs (imMSCs/eSDF-1−). NB rats induced by bilateral pelvic nerve (PN) transection were treated with imMSCs/eSDF-1+, imMSCs/eSDF-1−, or sham. After a 4-week treatment, the bladder function was assessed by cystometry and voiding pattern analysis. The PN and bladder tissues were evaluated via immunostaining and western blotting analysis. We found that imMSCs/eSDF-1+ expressed higher levels of SDF-1 in vitro and in vivo. The treatment of imMSCs/eSDF-1+ improved NB and evidently stimulated the recovery of bladder wall in NB rats. The recovery of injured nerve was more effective in the NB+imMSCs/eSDF-1+ group than in other groups. High SDF-1 expression improved the levels of vascular endothelial growth factor and basic fibroblast growth factor. Apoptosis was decreased after imMSCs injection, and was detected rarely in the NB+imMSCs/eSDF-1+ group. Injection of imMSCs boosted the expression of neuronal nitric oxide synthase, p-AKT, and p-ERK in the NB+imMSCs/eSDF-1+ group than in other groups. Our findings demonstrated that overexpression of SDF-1 induced additional MSC homing to the injured tissue, which improved the NB by accelerating the restoration of injured nerve in a rat model.

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Bladder wall injection of mesenchymal stem cells ameliorates bladder inflammation, overactivity and nociception in a chemically induced interstitial cystitis-like rat model

International Urogynecology Journal ◽

10.1007/s00192-018-3631-5 ◽

2018 ◽

Vol 30 (5) ◽

pp. 845-846

Author(s):

Dina El-Hamamsy

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Interstitial Cystitis ◽

Rat Model ◽

Bladder Wall ◽

Bladder Inflammation ◽

Chemically Induced ◽

Wall Injection

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Bladder wall injection of mesenchymal stem cells ameliorates bladder inflammation, overactivity, and nociception in a chemically induced interstitial cystitis-like rat model

International Urogynecology Journal ◽

10.1007/s00192-018-3592-8 ◽

2018 ◽

Vol 29 (11) ◽

pp. 1615-1622 ◽

Cited By ~ 4

Author(s):

Akira Furuta ◽

Tokunori Yamamoto ◽

Taro Igarashi ◽

Yasuyuki Suzuki ◽

Shin Egawa ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Interstitial Cystitis ◽

Rat Model ◽

Bladder Wall ◽

Bladder Inflammation ◽

Chemically Induced ◽

Wall Injection

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N48 TISSUE ENGINEERING OF THE URINARY BLADDER: TROPHIC EFFECTS OF MESENCHYMAL STEM CELLS IN RECONSTRUCTION OF THE RAT BLADDER WALL

European Urology Supplements ◽

10.1016/s1569-9056(10)61250-x ◽

2010 ◽

Vol 9 (6) ◽

pp. 546 ◽

Cited By ~ 1

Author(s):

M. Pokrywczynska ◽

A. Jundzill ◽

M. Bodnar ◽

J. Adamowicz ◽

A. Marszalek ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Urinary Bladder ◽

Bladder Wall ◽

Trophic Effects ◽

Rat Bladder

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485 Improvement of bladder fibrosis using direct transplantation of mesenchymal stem cells into bladder wall in rats with spinal cord injury

European Urology Supplements ◽

10.1016/s1569-9056(14)60478-4 ◽

2014 ◽

Vol 13 (1) ◽

pp. e485

Author(s):

Y.S. Song ◽

H.J. Lee ◽

J. An ◽

S.W. Doo ◽

J.H. Kim ◽

...

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Spinal Cord Injury ◽

Mesenchymal Stem Cells ◽

Bladder Wall ◽

Cord Injury

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Do Mesenchymal Stem Cells Modulate the Milieu of Reconstructed Bladder Wall?

Archivum Immunologiae et Therapiae Experimentalis ◽

10.1007/s00005-013-0249-7 ◽

2013 ◽

Vol 61 (6) ◽

pp. 483-493 ◽

Cited By ~ 21

Author(s):

Marta Pokrywczynska ◽

Arkadiusz Jundzill ◽

Magdalena Bodnar ◽

Jan Adamowicz ◽

Jakub Tworkiewicz ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bladder Wall

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Improved bladder contractility after transplantation of human mesenchymal stem cells overexpressing hepatocyte growth factor into underactive bladder from bladder outlet obstruction models of rats

PLoS ONE ◽

10.1371/journal.pone.0261402 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0261402

Author(s):

Jae Heon Kim ◽

Hee Jo Yang ◽

Sung Sik Choi ◽

Seung U. Kim ◽

Hong J. Lee ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Growth Factor ◽

Bladder Outlet Obstruction ◽

Bladder Wall ◽

Outlet Obstruction ◽

Human Mesenchymal Stem Cells ◽

Collagen Deposition ◽

Underactive Bladder ◽

Hepatocyte Growth

Introduction An underactive bladder can lead to difficulty in voiding that causes incomplete emptying of the bladder, suggesting the need for a new strategy to increase bladder contractility in such patients. This study was performed to investigate whether human mesenchymal stem cells (hMSCs) were capable of restoring bladder contractility in rats with underactive bladder due to bladder outlet obstruction (BOO) and enhancing their effects by overexpressing hepatocyte growth factor (HGF) in hMSCs. Materials and methods The hMSCs were transplanted into the bladder wall of rats. Fifty female Sprague-Dawley rats at six weeks of age were divided into five groups: group 1: control; group 2: sham intervention; group 3: eight-week BOO; group 4: BOO rats transplanted with hMSCs; and group 5: BOO rats transplanted with hMSCs overexpressing HGF. Two weeks after the onset of BOO in groups 4 and 5, hMSCs were injected into the bladder wall. Cystometry evaluation was followed by Masson’s trichrome staining of bladder tissues. Realtime PCR and immunohistochemical staining were performed to determine for hypoxia, apoptosis, and angiogenesis. Results Collagen deposition of bladder increased in BOO but decreased after transplantation of hMSCs. The increased inter-contraction interval and residual urine volume after BOO was reversed after hMSCs transplantation. The decreased maximal voiding pressure after BOO was restored by hMSCs treatment. The mRNA expression of bladder collagen1 and TGF-β1 increased in BOO but decreased after hMSCs transplantation. The decrease in vWF-positive cells in the bladder following BOO was increased after hMSCs transplantation. Caspase 3 and TUNEL-positive apoptosis of bladder cells increased in BOO but decreased after transplantation of hMSCs. These effects were enhanced by overexpressing HGF in hMSCs. Conclusion Transplantation of hMSCs into bladder wall increased the number of micro-vessels, decreased collagen deposition and apoptosis of detrusor muscle, and improved bladder underactivity. The effects were enhanced by overexpressing HGF in hMSCs. Our findings suggest that the restoration of underactive bladder using hMSCs may be used to rectify micturition disorders in patients following resolution of BOO. Further studies are needed before hMSCs can be used in clinical applications.

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