N48 TISSUE ENGINEERING OF THE URINARY BLADDER: TROPHIC EFFECTS OF MESENCHYMAL STEM CELLS IN RECONSTRUCTION OF THE RAT BLADDER WALL

2010 ◽  
Vol 9 (6) ◽  
pp. 546 ◽  
Author(s):  
M. Pokrywczynska ◽  
A. Jundzill ◽  
M. Bodnar ◽  
J. Adamowicz ◽  
A. Marszalek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Trophic Effects ◽  
Rat Bladder

664 TISSUE ENGINEERING OF THE URINARY BLADDER: TROPHIC EFFECTS OF MESENCHYMAL STEM CELLS IN RECONSTRUCTION OF THE RAT BLADDER WALL

2011 ◽  
Vol 10 (2) ◽  
pp. 214
Author(s):  
M. Pokrywczynska ◽  
A. Jundzill ◽  
M. Bodnar ◽  
J. Adamowicz ◽  
A. Marszalek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Trophic Effects ◽  
Rat Bladder

scholarly journals Tissue Engineering of Rat Bladder Using Marrow-Derived Mesenchymal Stem Cells and Bladder Acellular Matrix

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e111966 ◽  
Author(s):  
Daniel L. Coutu ◽  
Wally Mahfouz ◽  
Oleg Loutochin ◽  
Jacques Galipeau ◽  
Jacques Corcos
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Acellular Matrix ◽  
Rat Bladder

scholarly journals Tissue Engineering of the Urinary Bladder: Current Concepts and Future Perspectives

2011 ◽  
Vol 11 ◽  
pp. 1479-1488 ◽  
Author(s):  
Vladimir Petrovic ◽  
Jablan Stankovic ◽  
Vladisav Stefanovic
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Normal Structure ◽  
Frequent Relapses ◽  
Tissue Engineering Approach ◽  
Urinary Bladder Wall ◽  
Bladder Regeneration

There are many conditions that can affect the normal structure of the urinary bladder wall and lead to the inadequate evacuation of urine or even disable urine excretion. In these cases, the essential task is to restore the function of the urinary bladder, most often through surgical intervention. Some of the disorders, such as bladder acontractility, bladder cancer, and inflammatory disease, represent a great challenge in practice due to the number of complications that can occur after the intervention and due to frequent relapses. The use of tissue engineering strategies that include the use of stem cells and artificially created scaffolds could give solutions for treatment of many disorders of the urinary bladder and transplantation therapies in the future. Although the research in this field is still in its infancy, there are some promising results that raise hope that the tissue engineering approach could offer long-term solutions for many issues in regenerative urology. This review summarizes the current achievements and perspectives in the use of stem cells and tissue engineering techniques in the field of urinary bladder regeneration.

861 Use of bone marrow derived mesenchymal stem cells for autologous tissue engineering of urinary bladder muscularis

2004 ◽  
Vol 3 (2) ◽  
pp. 159 ◽  
Author(s):  
D. Schultheiss ◽  
A. Gabouev ◽  
A. Pilatz ◽  
J. Schanz ◽  
H. Mertsching ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Autologous Tissue

scholarly journals Bone Marrow-Derived Mesenchymal Stem Cells: Current and Future Applications in the Urinary Bladder

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Beth A. Drzewiecki ◽  
John C. Thomas ◽  
Stacy T. Tanaka
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Tissue Regeneration ◽  
Current Literature ◽  
Multiple Organ ◽  
Adult Tissue ◽  
Organ Systems

Mesenchymal stem cells can be isolated from almost any adult tissue. In this paper we focus on bone marrow-derived mesenchymal stem cells which have captured the interest of researchers since their introduction because of the promising potential of tissue regeneration and repair. They are known for their ability to self-renew and differentiate into diverse lineages while maintaining low immunogenicity. The exact mechanisms behind how these cells work still remain unclear, and there is a continuing shift in the paradigms that support them. There has been extensive research in multiple organ systems; however, the genitorurinary system has been vastly underrepresented. This article discusses the background behind bone marrow-derived mesenchymal stem cells and they are currently being applied to the urinary bladder in the realm of tissue engineering. We also postulate on their future applications based on the current literature in other organ systems.

Mesenchymal Stem Cells Differentiation: Mitochondria Matter in Osteogenesis or Adipogenesis Direction

2020 ◽  
Vol 15 (7) ◽  
pp. 602-606
Author(s):  
Kun Ji ◽  
Ling Ding ◽  
Xi Chen ◽  
Yun Dai ◽  
Fangfang Sun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Potential ◽  
Mesodermal Cell ◽  
Cell Lineages ◽  
Current Review ◽  
Differentiation Factors ◽  
The Relationship ◽  
Msc Differentiation

Mesenchymal Stem Cells (MSCs) exhibit enormous therapeutic potential because of their indispensable regenerative, reparative, angiogenic, anti-apoptotic, and immunosuppressive properties. MSCs can best differentiate into mesodermal cell lineages, including osteoblasts, adipocytes, muscle cells, endothelial cells and chondrocytes. Specific differentiation of MSCs could be induced through limited conditions. In addition to the relevant differentiation factors, drastic changes also occur in the microenvironment to conduct it in an optimal manner for particular differentiation. Recent evidence suggests that the mitochondria participate in the regulating of direction and process of MSCs differentiation. Therefore, our current review focuses on how mitochondria participate in both osteogenesis and adipogenesis of MSC differentiation. Besides that, in our current review, we try to provide a further understanding of the relationship between the behavior of mitochondria and the direction of MSC differentiation, which could optimize current cellular culturing protocols for further facilitating tissue engineering by adjusting specific conditions of stem cells.

The Effects of Mesenchymal Stem Cells in Craniofacial Tissue Engineering

2014 ◽  
Vol 9 (3) ◽  
pp. 280-289 ◽  
Author(s):  
Lin Zhang ◽  
Ge Feng ◽  
Xing Wei ◽  
Lan Huang ◽  
Aishu Ren ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Craniofacial Tissue

scholarly journals Fluid Flow Mechanical Stimulation-Assisted Cartridge Device for the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 927
Author(s):  
Ki-Taek Lim ◽  
Dinesh-K. Patel ◽  
Sayan-Deb Dutta ◽  
Keya Ganguly
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Flow ◽  
Osteogenic Differentiation ◽  
Flow Condition ◽  
Cultured Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Static Conditions

Human mesenchymal stem cells (hMSCs) have the potential to differentiate into different types of mesodermal tissues. In vitro proliferation and differentiation of hMSCs are necessary for bone regeneration in tissue engineering. The present study aimed to design and develop a fluid flow mechanically-assisted cartridge device to enhance the osteogenic differentiation of hMSCs. We used the fluorescence-activated cell-sorting method to analyze the multipotent properties of hMSCs and found that the cultured cells retained their stemness potential. We also evaluated the cell viabilities of the cultured cells via water-soluble tetrazolium salt 1 (WST-1) assay under different rates of flow (0.035, 0.21, and 0.35 mL/min) and static conditions and found that the cell growth rate was approximately 12% higher in the 0.035 mL/min flow condition than the other conditions. Moreover, the cultured cells were healthy and adhered properly to the culture substrate. Enhanced mineralization and alkaline phosphatase activity were also observed under different perfusion conditions compared to the static conditions, indicating that the applied conditions play important roles in the proliferation and differentiation of hMSCs. Furthermore, we determined the expression levels of osteogenesis-related genes, including the runt-related protein 2 (Runx2), collagen type I (Col1), osteopontin (OPN), and osteocalcin (OCN), under various perfusion vis-à-vis static conditions and found that they were significantly affected by the applied conditions. Furthermore, the fluorescence intensities of OCN and OPN osteogenic gene markers were found to be enhanced in the 0.035 mL/min flow condition compared to the control, indicating that it was a suitable condition for osteogenic differentiation. Taken together, the findings of this study reveal that the developed cartridge device promotes the proliferation and differentiation of hMSCs and can potentially be used in the field of tissue engineering.

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