Evaluation of Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva sylvestris and Capsella bursa-pastoris against Methicillin-Resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that becomes the main concern since it is a multidrug-resistant organism and causes high morbidity and mortality. This study aimed to investigate the antibacterial activity of ethanolic extract of Phaleria macrocarpa leaves, peel, and fruit flesh on MRSA. This study was an experimental laboratory study with a post-test only control group design to assess the antibacterial activity of ethanolic extract from leaves, peel, and fruit flesh of Phaleria macrocarpa against MRSA ATCC 43300 and MRSA clinical isolates using a disk diffusion method. Extracts from the leaves, peel, and flesh of Phaleria macrocarpa had potential as an antibacterial agent against MRSA ATCC 43300 at a concentration of 40%, although not yet equivalent to antibiotic control. The ethanol extract of Phaleria macrocarpa peel at a concentration of 30-40% had potential as an antibacterial agent against MRSA clinical isolates, although not yet equivalent to antibiotic control. Further research needs to be performed so that Phaleria macrocarpa extract can be a potential source of subsequent antibacterial development against MRSA.

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THE ANTIBIOFILM ACTIVITY OF HIBISCUS SABDRIFFA L. AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v52i3.1352 ◽

2021 ◽

Vol 52 (3) ◽

pp. 626-631

Author(s):

Jaddoa & Gharb

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Diffusion Method ◽

Antibiofilm Activity ◽

Dependent Manner ◽

Hibiscus Sabdariffa ◽

Methicillin Resistant

The current study was aimed to investigate the antibacterial and antibiofilm activity of ethanolic extract of Hibiscus sabdariffa L. calyx against locally isolated methicillin-resistant Staphylococcus aureus (MRSA) at different concentrations were started from 0.078 to 40 mg/ml. The extract was prepared by soaked calyces powder of Hibiscus sabdariffa L. with 80% ethanol in the Soxhlet extraction unit, and then it was aseptically filtered. The antibacterial activity was tested by agar diffusion method and broth microdilution method, this method was used to determine the minimum inhibitory concentration of extract, while the antibiofilm activity was determined by using 96-well polystyrene microtiter plates. The results revealed that the ethanolic extract has antibacterial activity in a concentration-dependent manner, the average diameter zone of inhibition observed against MRSA isolates ranged from 14±0.5 mm to 20±0.5 mm Moreover, at sub-inhibitory concentration, this extract developed an isolate-specific antibiofilm effect and presented highly significant (P< 0.05) variability in biofilm formation before and after addition of ethanolic extract, Mr1, and Mr7 isolates were gave the lowest and highest antibiofilm activity, respectively. In conclusion, the ethanolic extract of H. sabdariffa L. calyx is a promising alternative medication that can be used to treat the infection caused by MRS.

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Antibacterial Activity of Bandotan (Ageratum conyzoides L) Leaves Extracts Against Methicillin-Resistant Staphylococcus aureus

Borneo Journal of Pharmacy ◽

10.33084/bjop.v3i4.1552 ◽

2020 ◽

Vol 3 (4) ◽

pp. 243-248

Author(s):

Selvira Anandia Intan Maulidya ◽

Doni Anshar Nuari ◽

Shendi Suryana ◽

Sumia Almarifah

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Ethanolic Extract ◽

Dilution Method ◽

Agar Dilution Method ◽

Ageratum Conyzoides ◽

Methicillin Resistant ◽

Ethanolic Extracts ◽

Mic Value

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections throughout the world and can be life-threatening as well. This study aimed to determine the antibacterial activity of Bandotan (Ageratum conyzoides L) leaves ethanolic extract against MRSA's growth. Ageratum conyzoides leaves were extracted by ethanol and screened for their phytochemical constituent. Ethanolic extracts of A. conyzoides leaves were evaluated for their potential antibacterial activity using disc diffusion assay. The minimum inhibitory concentration (MIC) value was determined using the agar dilution method. Phytochemical screening shows that the extracts contain alkaloids, flavonoids, saponins, tannins, and steroids or triterpenoids. Ageratum conyzoides leaves extract shows a 25.1 mm inhibitory zone at 12.5% extract concentration with MIC value equivalents to 4.46 x 10-6 g of gentamicin. This study concludes that A. conyzoides leaves ethanolic extracts have potential antibacterial activity against MRSA.

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