scholarly journals Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) of Ethanol Extract of God's Crown (Phaleria macrocarpa) Leaves, Peel, and Fruit Flesh

2020 ◽  
Vol 31 (2) ◽  
pp. 81
Author(s):  
Rani Afifah Nur Hestiyani ◽  
Tri Okmawati Handini
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Methicillin Resistant ◽  
Fruit Flesh ◽  
Phaleria Macrocarpa ◽  
Antibiotic Control

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that becomes the main concern since it is a multidrug-resistant organism and causes high morbidity and mortality. This study aimed to investigate the antibacterial activity of ethanolic extract of Phaleria macrocarpa leaves, peel, and fruit flesh on MRSA. This study was an experimental laboratory study with a post-test only control group design to assess the antibacterial activity of ethanolic extract from leaves, peel, and fruit flesh of Phaleria macrocarpa against MRSA ATCC 43300 and MRSA clinical isolates using a disk diffusion method. Extracts from the leaves, peel, and flesh of Phaleria macrocarpa had potential as an antibacterial agent against MRSA ATCC 43300 at a concentration of 40%, although not yet equivalent to antibiotic control. The ethanol extract of Phaleria macrocarpa peel at a concentration of 30-40% had potential as an antibacterial agent against MRSA clinical isolates, although not yet equivalent to antibiotic control. Further research needs to be performed so that Phaleria macrocarpa extract can be a potential source of subsequent antibacterial development against MRSA.

Synthesis and antibacterial activity evaluation of C-5 side chain modified analogues of FYL-66, a potential agent against methicillin-resistant Staphylococcus aureus

MedChemComm ◽  
2015 ◽  
Vol 6 (6) ◽  
pp. 1156-1172 ◽  
Author(s):  
Xiaoyan Yang ◽  
Zicheng Li ◽  
Zhenling Wang ◽  
Zitai Sang ◽  
Haiyue Long ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Animal Models ◽  
Antibacterial Agent ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Biological Activities ◽  
Side Chain ◽  
Methicillin Resistant ◽  
Activity Evaluation

Monofluoro- (39), difluoro- (40) and trifluoro- (41) substituted analogues showed different biological activities, and 40 is a potent antibacterial agent in animal models.

scholarly journals In vitro Activity of Rambutan Binjai (Nephelium lappaceum) Peel Extract from Indonesia to Methicillin-Resistant Staphylococcus aureus (MRSA)

2018 ◽  
Vol 17 (2) ◽  
pp. 197-203
Author(s):  
Tina Rostinawati ◽  
Ami Tjitraresmi ◽  
Myra Vania Wisnuputri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Nephelium Lappaceum ◽  
New Compounds ◽  
Peel Extract

Methicillin-resistant Staphylococcus aureus (MRSA) is the most common bacteria causing nosocomial infections with high levels of resistance to available antibiotics. So, it is necessary to search for new compounds to solve this problem. Various studies showed antibacterial activity of rambutan peel but for Rambutan Binjai peel extract that are from Indonesia has never been studied against the MRSA. This study aims to determine the antibacterial activity, the value of minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) using agar diffusion method. The concentration of rambutan peel ethanol extract at as much as 62.5 mg/ml showed the inhibitory diameter i.e 21.3 ± 2.4 mm. MIC and MBC were in the same range, which was between 0.98 (mg/ml) to 1.95 (mg/ml). The activity strength of tetracycline against the extract was at 1:50. This revealed that Rambutan Binjai peel extract had great potency as antibacterial agent to MRSA. Dhaka Univ. J. Pharm. Sci. 17(2): 197-203, 2018 (December)

scholarly journals Antibacterial activity testing of ethanolic extract of aloe vera leaf and gel against methicillin-resistant Staphylococcus aureus

2014 ◽  
Vol 26 (2) ◽  
Author(s):  
Tias Eka Pratiwi ◽  
Warta Dewi ◽  
Eddy Prijono
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Test Method ◽  
Methicillin Resistant ◽  
Aloe Vera Gel ◽  
Significant Difference

Introduction: The interest on Aloe vera based herbal products is fast growing during recent years, particularly in medication regarding their antibacterial properties which had a lot of active components such as saponin and anthraquinone. Methicillin-resistant Staphylococcus aureus has emerged as one of the most important human pathogens causing nosocomial infections and became the first priority as a consequence of their resistancy. The purpose of this study is to determine the minimum levels of ethanol extract of leaves and Aloe Vera gel that can inhibit MRSA and find out whether there are differences in antibacterial power between of ethanol extract of leaves and Aloe Vera gel. Methods: Ten isolates of MRSA were investigated for their sensitivity to Aloe vera leaf and gel extract using the serial dilution method by doing two times repetition and statistically analyzed according to t-test method. Result: The result shows that the Minimum Inhibitory Concentration of Aloe vera leaf and gel extract each located at a concentration of 25% and 12,5%. There is a significant difference in antibacterial power between the ethanol extract of leaves and Aloe Vera gel in inhibiting MRSA with MIC values of 25% and 12.5% Conclusion: There was a difference between Aloe vera leaf and gel extract antibacterial activity, where the gel extract is more effective than leaf extract.

IDENTIFICATION OF CHEMICAL COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF 96% ETHANOL EXTRACT FROM MORINGA OLEIFERA LAM. LEAVES AGAINST MRSA (METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS)

2021 ◽  
pp. 111-114
Author(s):  
NURSANNA IRAWATY SINAGA ◽  
MUHAMMAD HANAFI ◽  
NOVI YANTIH
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Methicillin Resistant ◽  
Activity Test ◽  
Moringa Oleifera Lam

Objective: Infection with antibiotic-resistant organisms, requiring the selection of the right drug to fight these organisms. Moringa oleifera Lam. leaves as natural ingredients have MRSA (Methicillin-Resistant Staphylococcus aureus) antibacterial activity. The purpose of this study is to identify the chemical compounds and to determine the antibacterial activity from the 96% ethanol extract of Moringa oleifera Lam. leaves against MRSA bacteria. Methods: The Moringa oleifera Lam. leaves were extracted by maceration using 96% (v/v) ethanol. Mass spectrometry was performed on an LC-MS/MS Xevo, G2-XS QTof (Waters MS Technologies) to identified chemical compounds from the extract. The ionization type is ESI. The method of the antibacterial activity test was using agar paper disc diffusion. Antibacterial activity was based on the diameter of the bacterial inhibition zone. Results: The result of the antibacterial activity test for 96% ethanol extract of Moringa oleifera Lam. leaves a concentration of 10, 20, and 40 % each has inhibition diameter was 10, 13, and 15.5 mm, and for linezolid as a positive control at 30µg has diameter inhibition was 20 mm, ethanol 96% as a negative control was 0 mm. The 96% ethanol extract of Moringa oleifera Lam. leaves contains 13-hydroxy-9,11-hexadecadienoic acid, 3-tert-butyl-4-methoxyphenol, bistortaside, daturametelin H, digiprolactone, ephedradine C, kaempferol-3-O-rutinoside,kaempferol-3-O-β-D-glucopyranoside, kaempferol-7-O-α-L rhamnoside, phenyl propionic acid, pyrophaeophorbide A, quercetin, stearidonic acid, stigmastan-3,6-dione. Conclusion: The 96% ethanol extract of Moringa oleifera Lam. leaves contains 14 compounds. The 96% ethanol extract of Moringa oleifera Lam. leaves have activity against MRSA bacteria. The antibacterial effect of the extract increased with an increase in its concentration. The extract exerted a greater antibacterial effect on the concentration 40%.

scholarly journals Antibacterial Activity of Ethanolic Extract of Kitolod (Hippobromalongiflora) Leaf Against Staphylococcus aureus and Salmonella typhi

2020 ◽  
Vol 8 (1) ◽  
pp. 52-54 ◽  
Author(s):  
Simanjuntak Helen Anjelina
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Standard Protocol ◽  
Phytochemical Screening

Objective: The purpose of this study was to determine at what concentration of ethanol extract of kitolod leaves is active against Staphylococcus aureus and Salmonella typhi. Methods: Ethanolic extract of Kitolod leaves was tested for phytochemical screening by using standard protocol. Antibacterial testing was using the diffusion disc method to measure the inhibition zone against the Staphylococcus aureus and Salmonella typhi with various concentration of Kitolod leaves extract (6.25%, 12.5%, 25%, 50%, and 75%). Results: Phytochemical screening showed that ethanolic extract of Kitolod leaves contain alkaloids, flavonoids and saponins. The antibacterial inhibition of ethanol extract of kitolod leaves against Staphylococcus aureus and Salmonella typhi bacteria at a concentration of 75% had a diameter of 11.3 mm and 12.16 mm with a strong category. Conclusions: Kitolod leaf could be use as a novel antibacterial agent.    

scholarly journals THE ANTIBIOFILM ACTIVITY OF HIBISCUS SABDRIFFA L. AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 52 (3) ◽  
pp. 626-631
Author(s):  
Jaddoa & Gharb
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Dependent Manner ◽  
Hibiscus Sabdariffa ◽  
Methicillin Resistant

The current study was aimed to investigate the antibacterial and antibiofilm activity of ethanolic extract of Hibiscus sabdariffa L. calyx against locally isolated methicillin-resistant Staphylococcus aureus (MRSA) at different concentrations were started from 0.078 to 40 mg/ml. The extract was prepared by soaked calyces powder of Hibiscus sabdariffa L. with 80% ethanol in the Soxhlet extraction unit, and then it was aseptically filtered. The antibacterial activity was tested by agar diffusion method and broth microdilution method, this method was used to determine the minimum inhibitory concentration of extract, while the antibiofilm activity was determined by using 96-well polystyrene microtiter plates. The results revealed that the ethanolic extract has antibacterial activity in a concentration-dependent manner, the average diameter zone of inhibition observed against MRSA isolates ranged from 14±0.5 mm to 20±0.5 mm Moreover, at sub-inhibitory concentration, this extract developed an isolate-specific antibiofilm effect and presented highly significant (P< 0.05) variability in biofilm formation before and after addition of ethanolic extract, Mr1, and Mr7 isolates were gave the lowest and highest antibiofilm activity, respectively. In conclusion, the ethanolic extract of H. sabdariffa L. calyx is a promising alternative medication that can be used to treat the infection caused by MRS.

scholarly journals Antibacterial Activity of Bandotan (Ageratum conyzoides L) Leaves Extracts Against Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 3 (4) ◽  
pp. 243-248
Author(s):  
Selvira Anandia Intan Maulidya ◽  
Doni Anshar Nuari ◽  
Shendi Suryana ◽  
Sumia Almarifah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Ageratum Conyzoides ◽  
Methicillin Resistant ◽  
Ethanolic Extracts ◽  
Mic Value

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections throughout the world and can be life-threatening as well. This study aimed to determine the antibacterial activity of Bandotan (Ageratum conyzoides L) leaves ethanolic extract against MRSA's growth. Ageratum conyzoides leaves were extracted by ethanol and screened for their phytochemical constituent. Ethanolic extracts of A. conyzoides leaves were evaluated for their potential antibacterial activity using disc diffusion assay. The minimum inhibitory concentration (MIC) value was determined using the agar dilution method. Phytochemical screening shows that the extracts contain alkaloids, flavonoids, saponins, tannins, and steroids or triterpenoids. Ageratum conyzoides leaves extract shows a 25.1 mm inhibitory zone at 12.5% extract concentration with MIC value equivalents to 4.46 x 10-6 g of gentamicin. This study concludes that A. conyzoides leaves ethanolic extracts have potential antibacterial activity against MRSA.

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