scholarly journals Perinatal Al-Quran Sound to Novel Object Recognition Memory and Hippocampal Cell Count

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Tryando Bhatara ◽  
Achadiyani Achadiyani ◽  
Uni Gamayani ◽  
Herry Herman
Keyword(s):  
Object Recognition ◽  
Cell Survival ◽  
Wistar Rats ◽  
Hippocampal Formation ◽  
Stress Factors ◽  
Cell Counting ◽  
Novel Object Recognition ◽  
Novel Object ◽  
Hematoxylin Eosin

Al-Quran sound as auditory stimulation may influence the development of systems related to memory. This study aimed to investigate the effect of Al-Quran acoustic stimulation to novel object recognition (NOR) memory and amount of hippocampal formation cells at postnatal day (PND) 21 Wistar rats. This study was conducted in September 2016 to January 2017. Adult Wistar rats divided into intervention and control groups were bred at Faculty of Medicine Universitas Islam Bandung. Rat pups in the intervention group were exposed to Quranic sound from postcoital day 0 to the PND 20. Rats of PND 20 was involved in the NOR test by documenting the value of the duration of exploration of the familiar and novel object. The rats’ brains were extracted and processed at Faculty of Medicine Universitas Padjadjaran for cell counting of hippocampal formation stained with hematoxylin-eosin. The results displayed a higher value of D1 (exploration time difference) and a total number of hippocampal formation cells in the Al-Quran groups. These results can be related to the role of the Quranic voice in suggesting higher learning aspects, activating neurogenesis or cell survival transcription factors. However, there was no difference in discrimination index (DI) value between groups which could be indicating inadequate habituation period, interval, testing age, or stress factors. Numerous limitations from this field of research suggest that the biological role of sound stimulation is still in its early stages of development. In conclusion, exposure to perinatal Al-Quran sound may serve as stimulation which enhances learning, memory, neurogenesis or cell survival of hippocampal formation. SUARA AL-QURAN PERINATAL PADA MEMORI NOVEL OBJECT RECOGNITION DAN JUMLAH SEL HIPOKAMPUSSuara Al-Quran sebagai stimulasi pendengaran diperkirakan dapat memengaruhi perkembangan sistem tubuh terkait memori. Penelitian ini bertujuan mengamati pengaruh stimulasi suara Al-Quran terhadap memori novel object recognition (NOR) dan jumlah sel formasi hipokampus pada tikus Wistar 21 hari setelah lahir (postnatal day/PND). Penelitian ini dilaksanakan pada September 2016 sampai Januari 2017. Tikus Wistar dewasa yang dibagi dalam kelompok perlakuan dan kontrol dibiakkan di Fakultas Kedokteran Universitas Islam Bandung. Anak tikus Wistar dipaparkan dengan suara Al-Quran sejak hari postcoital 0 sampai anak tikus lahir dan berumur 20 hari setelah lahir (PND 20). Tikus PND 20 dilibatkan dalam tes NOR dengan dokumentasi nilai durasi eksplorasi objek lama dan baru. Otak tikus diproses di Fakultas Kedokteran Universitas Padjadjaran untuk penghitungan jumlah sel formasi hipokampus dengan pewarnaan hematoxylin-eosin. Hasil penelitian ini menunjukkan nilai D1 (perbedaan durasi eksplorasi objek lama–baru) dan jumlah sel formasi hipokampus lebih tinggi pada kelompok perlakuan dengan Al-Quran. Hasil tersebut dapat terkait dengan peran suara Al-Quran dalam aktivasi faktor pertumbuhan atau transkripsi. Namun, tidak terdapat perbedaan bermakna pada nilai indeks diskriminasi antarkelompok yang dapat terkait dengan faktor stres atau kurangnya periode habituasi atau periode uji. Berbagai keterbatasan penelitian ini serta riset di bidang stimulasi embriologi mengindikasikan bahwa peran biologis suara Al-Quran masih harus diteliti lebih lanjut. Simpulan, paparan suara Al-Quran perinatal dapat berlaku sebagai stimulasi yang meningkatkan pembelajaran, memori, neurogenesis atau ketahanan sel formasi hipokampus.

scholarly journals Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway

2020 ◽  
Author(s):  
Fan Ye ◽  
Shiming Tian ◽  
Huimin Hu ◽  
Zhengwen Yu
Keyword(s):  
Object Recognition ◽  
Treatment Efficacy ◽  
Memory Function ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Novel Object Recognition ◽  
Quantitative Real Time Pcr ◽  
Novel Object ◽  
Underlying Mechanisms

Abstract Background: The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored. Methods: Quantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats. Results: Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.Conclusion: In summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.

scholarly journals Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway

2020 ◽  
Author(s):  
Fan Ye ◽  
Shiming Tian ◽  
Huimin Hu ◽  
Zhengwen Yu
Keyword(s):  
Object Recognition ◽  
Treatment Efficacy ◽  
Memory Function ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Novel Object Recognition ◽  
Quantitative Real Time Pcr ◽  
Novel Object ◽  
Underlying Mechanisms

Abstract Background: The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored. Methods: Quantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats. Results: Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats. Conclusion: In summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.

The role of apelin-13 in novel object recognition memory

Peptides ◽  
2014 ◽  
Vol 62 ◽  
pp. 155-158 ◽  
Author(s):  
Ren-wen Han ◽  
Hong-jiao Xu ◽  
Rui-san Zhang ◽  
Rui Wang
Keyword(s):  
Object Recognition ◽  
Recognition Memory ◽  
Novel Object Recognition ◽  
Novel Object ◽  
Object Recognition Memory

scholarly journals The Role of Gut Microbiota in Chronic Itch-Evoked Novel Object Recognition-Related Cognitive Dysfunction in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Yujuan Li ◽  
Wencui Zhang ◽  
Tainning Sun ◽  
Baowen Liu ◽  
Anne Manyande ◽  
...  
Keyword(s):  
Object Recognition ◽  
Gut Microbiota ◽  
Cognitive Dysfunction ◽  
Rrna Gene ◽  
Novel Object Recognition ◽  
Microbiota Composition ◽  
Object Recognition Test ◽  
Novel Object ◽  
Chronic Itch

The high incidence of patients with chronic itch highlights the importance of fundamental research. Recent advances in the interface of gut microbiota have shed new light into exploring this phenomenon. However, it is unknown whether gut microbiota plays a role in chronic itch in rodents with or without cognitive dysfunction. In this study, the role of gut microbiota in diphenylcyclopropenone (DCP)-evoked chronic itch was investigated in mice and hierarchical cluster analysis of novel object recognition test (ORT) results were used to classify DCP-evoked itch model in mice with or without cognitive dysfunction (CD)-like phenotype and 16S ribosomal RNA (rRNA) gene sequencing was used to compare gut bacterial composition between CD (Susceptible) and Non-CD phenotypes (Unsusceptible) in chronic itch mice. Results showed that the microbiota composition was significantly altered by DCP-evoked chronic itch and chronic itch induced novel object recognition-related CD. However, abnormal gut microbiota composition induced by chronic itch may not be correlated with novel object recognition-related CD.

Novel object recognition memory in REM sleep-deprived rats: Role of the cannabinoid CB1 receptor

2020 ◽  
Vol 381 ◽  
pp. 112311 ◽  
Author(s):  
Kaveh Shahveisi ◽  
Vahid Farnia ◽  
Habibolah Khazaie ◽  
Hamed Ghazvini ◽  
Masoumeh Nozari ◽  
...  
Keyword(s):  
Object Recognition ◽  
Recognition Memory ◽  
Rem Sleep ◽  
Cb1 Receptor ◽  
Novel Object Recognition ◽  
Cannabinoid Cb1 Receptor ◽  
Novel Object ◽  
Object Recognition Memory

scholarly journals Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway

2020 ◽  
Author(s):  
Fan Ye ◽  
Shiming Tian ◽  
Huimin Hu ◽  
Zhengwen Yu
Keyword(s):  
Object Recognition ◽  
Treatment Efficacy ◽  
Memory Function ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Novel Object Recognition ◽  
Quantitative Real Time Pcr ◽  
Novel Object ◽  
Underlying Mechanisms

Abstract Background: The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored.Methods: Quantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.Results: Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.Conclusion: In summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.

Sign in / Sign up

Export Citation Format

Share Document