The concentration of dissolved oxygen (DO) and organics in biological aerated filters (BAFs) changes with the height. Due to the different microbiologic property distributions in different heights, biomass and bioactivity also vary with the height. By monitoring biomembranes at different heights in a laboratory simulation reactor, we found that owing to the decrease of organic substance concentration, biomass decreased with the change of height; bioactivity showed V shaped changes with the height, and due to the influence of intercepted suspended substance, bioactivity in the middle and lower levels were the lowest. Observation on the biomembrane biofacies showed that biomembrane became thinner along with the flow direction, i.e. from 700μm to 100μm, and the colour became lighter. Biofacies changed from lower level to higher level, forming a complete biosystem.