Fed-batch cultures of recombinant Escherichia coli with inhibitory substance concentration monitoring

1988 ◽  
Vol 66 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Norio Shimizu ◽  
Shinichi Fukuzono ◽  
Kiyoshi Fujimori ◽  
Nabuko Nishimura ◽  
Yoji Odawara
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Inhibitory Substance ◽  
Batch Cultures ◽  
Substance Concentration

Production of Thermophilic Protease (Aqualysin I) by Fed-Batch Cultures of Recombinant Escherichia coli

1992 ◽  
pp. 192-194
Author(s):  
Shinji Sakamoto ◽  
Kazumasa Tsuji ◽  
Kyoko Uehara ◽  
Michiko Aoki ◽  
Ichiro Terada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Batch Cultures

Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

2009 ◽  
Vol 14 (5) ◽  
pp. 559-564 ◽  
Author(s):  
Jung Hun Kim ◽  
Seon-Won Kim ◽  
Do Quynh Anh Nguyen ◽  
He Li ◽  
Sung Bae Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mevalonate Pathway ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Batch Cultures ◽  
Β Carotene

Production of α-Amylase in Fed-Batch Cultures of vgb+ and vgb- Recombinant Escherichia coli: Some Observations

1999 ◽  
Vol 15 (4) ◽  
pp. 640-645 ◽  
Author(s):  
N. Enayati ◽  
C. Tari ◽  
S.J. Parulekar ◽  
B.C. Stark ◽  
D.A. Webster
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Batch Cultures

scholarly journals Elimination of d-Lactate Synthesis Increases Poly(3-Hydroxybutyrate) and Ethanol Synthesis from Glycerol and Affects Cofactor Distribution in Recombinant Escherichia coli

2010 ◽  
Vol 76 (22) ◽  
pp. 7400-7406 ◽  
Author(s):  
Pablo I. Nikel ◽  
Andrea M. Giordano ◽  
Alejandra de Almeida ◽  
Manuel S. Godoy ◽  
M. Julia Pettinari
Keyword(s):  
Escherichia Coli ◽  
Reducing Power ◽  
Carbon Partitioning ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Acetate Production ◽  
Batch Cultures ◽  
Metabolic Products ◽  
Ethanol Synthesis

ABSTRACT The effect of eliminating d-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g·liter−1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g·g−1, the highest reported using this substrate.

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