Effect of coconut oil on Anopheles gambiae sensu lato (Diptera: Culicidae) larvae tolerance in malaria vector control in Dogbo district in south-western Benin, West Africa

The use of chemical insecticides causes important damages to environment and human health and there is a need to search for alternative solutions. This study aims to investigate on the effect of coconut oil on Anopheles gambiae sensu lato larvae tolerance in malaria vector control in Dogbo district in south-western Benin, West Africa. Larvae of Anopheles gambiae s.l. mosquitoes were collected from breeding sites using the dipping method in May 2020 during the rainy season in Dogbo district. A batch of 25 larvae of fourth instar were exposed to a mixture of coconut oil with distilled water saturated with oxygen containing in each of five glass jars or test cups of same dimensions contained each 48 ml distilled water saturated with oxygen plus 2 ml of coconut oil and one control jar containing no trace of coconut oil. Larval mortality was recorded after 24 hours, 48 hours and 72hours exposure. The results show that the use of coconut oil causes full-grown Anopheles larvae to die by suffocation. After the application of this mixture, the larvae of four instars cannot breathe. The use of coconut oil is effective method for disturbing the siphonal respiration of mosquito larvae. Coconut oil is effective method for mosquito larvae control.

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Effect of aqueous extract of lemon (Citrus limon) on Anopheles gambiae sensu lato (Diptera: Culicidae) larvae tolerance in malaria vector control in Dogbo district in south-western Republic of Benin, West Africa

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2021.17.2.0292 ◽

2021 ◽

Vol 17 (2) ◽

pp. 001-007

Author(s):

Nazaire Aïzoun ◽

Kourouma Koura ◽

Arlette Adjatin

Keyword(s):

West Africa ◽

Anopheles Gambiae ◽

Aqueous Extract ◽

Larval Mortality ◽

Citrus Limon ◽

Mosquito Larvae ◽

Distilled Water ◽

Anopheles Gambiae S.L ◽

Republic Of Benin ◽

Water Saturated

The use of chemical insecticides causes important damages to environment and human health and there is a need to search for alternative solutions. This study aims to investigate on the effect of aqueous extract of lemon in Anopheles gambiae s.l. larvae tolerance in couffo department in south-western Republic of Benin, West Africa. Larvae of Anopheles gambiae s.l mosquitoes were collected from breeding sites using the dipping method in July 2020 during the rainy season in Dogbo district. A batch of 15 larvae of fourth instar were exposed to a mixture of aqueous extract of lemon with distilled water saturated with oxygen containing in each of five glass jars or test cups of same dimensions contained each 48 ml distilled water saturated with oxygen plus 2 ml of aqueous extract of lemon (Citrus limon) and one control jar containing no trace of aqueous extract of lemon. Larval mortality was recorded after 24hours, 48hours and 72hours exposure. The results show that the use of aqueous extract of lemon causes full-grown Anopheles larvae to die by suffocation. After the application of this mixture, the larvae of four instars cannot breathe. The use of aqueous extract of lemon is effective method for disturbing the siphonal respiration of mosquito larvae. Aqueous extract of lemon is effective method for mosquito larvae control.

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Bendiocarb resistance in Anopheles gambiae s.l. populations from Atacora department in Benin, West Africa: a threat for malaria vector control

Parasites & Vectors ◽

10.1186/1756-3305-6-192 ◽

2013 ◽

Vol 6 (1) ◽

Cited By ~ 38

Author(s):

Rock Aïkpon ◽

Fiacre Agossa ◽

Razaki Ossè ◽

Olivier Oussou ◽

Nazaire Aïzoun ◽

...

Keyword(s):

West Africa ◽

Vector Control ◽

Anopheles Gambiae ◽

Malaria Vector ◽

Malaria Vector Control ◽

Anopheles Gambiae S.L ◽

Bendiocarb Resistance

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Insecticide resistance in Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) and Anopheles gambiae Giles (Diptera: Culicidae) could compromise the sustainability of malaria vector control strategies in West Africa

Acta Tropica ◽

10.1016/j.actatropica.2013.06.004 ◽

2013 ◽

Vol 128 (1) ◽

pp. 7-17 ◽

Cited By ~ 22

Author(s):

Olivier Gnankiné ◽

Imael H.N. Bassolé ◽

Fabrice Chandre ◽

Isabelle Glitho ◽

Martin Akogbeto ◽

...

Keyword(s):

West Africa ◽

Insecticide Resistance ◽

Vector Control ◽

Anopheles Gambiae ◽

Bemisia Tabaci ◽

Malaria Vector ◽

Control Strategies ◽

Malaria Vector Control ◽

Anopheles Gambiae Giles

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An island within an island: genetic differentiation of Anopheles gambiae in São Tomé, West Africa, and its relevance to malaria vector control

Heredity ◽

10.1038/sj.hdy.6800348 ◽

2003 ◽

Vol 91 (4) ◽

pp. 407-414 ◽

Cited By ~ 22

Author(s):

J Pinto ◽

M J Donnelly ◽

C A Sousa ◽

J Malta-Vacas ◽

V Gil ◽

...

Keyword(s):

Genetic Differentiation ◽

West Africa ◽

Vector Control ◽

Anopheles Gambiae ◽

Malaria Vector ◽

Malaria Vector Control

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Systematic identification of plausible pathways to potential harm via problem formulation for investigational releases of a population suppression gene drive to control the human malaria vector Anopheles gambiae in West Africa

Malaria Journal ◽

10.1186/s12936-021-03674-6 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

John B. Connolly ◽

John D. Mumford ◽

Silke Fuchs ◽

Geoff Turner ◽

Camilla Beech ◽

...

Keyword(s):

West Africa ◽

Vector Control ◽

Anopheles Gambiae ◽

Malaria Vector ◽

Problem Formulation ◽

Malaria Vector Control ◽

Mosquito Vector ◽

Gene Drive ◽

Potential Harm ◽

Population Suppression

Abstract Background Population suppression gene drive has been proposed as a strategy for malaria vector control. A CRISPR-Cas9-based transgene homing at the doublesex locus (dsxFCRISPRh) has recently been shown to increase rapidly in frequency in, and suppress, caged laboratory populations of the malaria mosquito vector Anopheles gambiae. Here, problem formulation, an initial step in environmental risk assessment (ERA), was performed for simulated field releases of the dsxFCRISPRh transgene in West Africa. Methods Building on consultative workshops in Africa that previously identified relevant environmental and health protection goals for ERA of gene drive in malaria vector control, 8 potentially harmful effects from these simulated releases were identified. These were stratified into 46 plausible pathways describing the causal chain of events that would be required for potential harms to occur. Risk hypotheses to interrogate critical steps in each pathway, and an analysis plan involving experiments, modelling and literature review to test each of those risk hypotheses, were developed. Results Most potential harms involved increased human (n = 13) or animal (n = 13) disease transmission, emphasizing the importance to subsequent stages of ERA of data on vectorial capacity comparing transgenics to non-transgenics. Although some of the pathways (n = 14) were based on known anatomical alterations in dsxFCRISPRh homozygotes, many could also be applicable to field releases of a range of other transgenic strains of mosquito (n = 18). In addition to population suppression of target organisms being an accepted outcome for existing vector control programmes, these investigations also revealed that the efficacy of population suppression caused by the dsxFCRISPRh transgene should itself directly affect most pathways (n = 35). Conclusions Modelling will play an essential role in subsequent stages of ERA by clarifying the dynamics of this relationship between population suppression and reduction in exposure to specific potential harms. This analysis represents a comprehensive identification of plausible pathways to potential harm using problem formulation for a specific gene drive transgene and organism, and a transparent communication tool that could inform future regulatory studies, guide subsequent stages of ERA, and stimulate further, broader engagement on the use of population suppression gene drive to control malaria vectors in West Africa.

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