Ultrastructural evaluation of oocytes during fluorosis in rat ovarian follicles

Fluoride is a well-known environmental pollutant and its effect on human health has long been of interest to biomedical researchers. Various studies have shown that fluoride causes adverse effects on the fertility. Wistar albino female rats weighing 150-200 g were randomly divided into six rats in each group. The rats in experimental groups treated with 300 and 600 mg NaF/kg bw/day by oral gavage for 40 days. The present investigation focuses on the ovary of rat treated with sodium fluoride and its amelioration by curcumin. The results revealed that the sodium fluoride exposure to female rats treated with 300 mg NaF/kg bw/day, the surface epithelium had less number of ruffles and blebs of the plasma membrane. There was abrasion of ovarian surface epithelium. In rats treated with 600 mg/kg bw/day NaF, the cuboidal shape of the surface epithelium were changed into elongated appearance. The cells were devoid of microvilli, blebs and ruffles. After administration of curcumin, many follicles in different stages of development were visible. The ovarian surface epithelium showed normal surface epithelium with improvement in the shape of cuboidal cells.

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Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From proliferating cell nuclear antigen and caspase-3 perspectives

Veterinarski glasnik ◽

10.2298/vetgl180320007r ◽

2018 ◽

Vol 72 (2) ◽

pp. 80-89

Author(s):

Anita Radovanovic ◽

Milica Kovacevic-Filipovic ◽

Ivan Milosevic ◽

Tijana Luzajic ◽

Stefan Velickovic ◽

...

Keyword(s):

Proliferating Cell Nuclear Antigen ◽

Caspase 3 ◽

Ovarian Surface Epithelium ◽

Female Rats ◽

Nuclear Antigen ◽

Surface Epithelium ◽

Ovarian Surface ◽

Cell Height ◽

The Impact ◽

Proliferating Cell

Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodeling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period on morphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer.

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Faculty Opinions recommendation of β-catenin/Tcf-signaling appears to establish the murine ovarian surface epithelium (OSE) and remains active in selected postnatal OSE cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717447891.792902851 ◽

2012 ◽

Author(s):

Albert Ricken

Keyword(s):

Ovarian Surface Epithelium ◽

Surface Epithelium ◽

Ovarian Surface

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Induction of Proliferation in the Primate Ovarian Surface Epithelium In Vivo

Obstetrical & Gynecological Survey ◽

10.1097/01.ogx.0000312153.85442.13 ◽

2008 ◽

Vol 63 (5) ◽

pp. 307-308

Author(s):

Jay W. Wright ◽

Tanja Pejovic ◽

John Fanton ◽

Richard L. Stouffer

Keyword(s):

Ovarian Surface Epithelium ◽

Surface Epithelium ◽

Ovarian Surface

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Efficient introduction of genes into human ovarian surface epithelium

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-997-0120-4 ◽

1997 ◽

Vol 33 (9) ◽

pp. 665-667

Author(s):

Earnest H. Leung ◽

Barry R. Davies ◽

Peter C. K. Leung ◽

George T. C. Chen ◽

Calvin D. Roskelley ◽

...

Keyword(s):

Ovarian Surface Epithelium ◽

Surface Epithelium ◽

Ovarian Surface

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Isolation and Characterization of Mesenchymal Stem Cells from Canine Ovarian Surface Epithelium

Indian Journal of Animal Research ◽

10.18805/ijar.b-4494 ◽

2021 ◽

Author(s):

Ajeet Kumar Jha ◽

Anirban Mandal ◽

Kalyani Ray ◽

Shyamal Kanti Guha

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stromal Vascular Fraction ◽

Ovarian Surface Epithelium ◽

Surface Epithelium ◽

High Plasticity ◽

Rt Pcr ◽

Differentiation Potential ◽

Ovarian Surface ◽

Isolation And Characterization

Background: Few studies have confirmed the presence of ovarian tissue stem cells indicating the capacity for differentiation. Based on this fact, it was hypothesized that mesenchymal stem cells (MSC) were found in ovarian surface epithelium (OSE) of canines that could easily be isolated. Methods: Both left and right ovaries were minced and digested using collagenase to obtain a stromal vascular fraction (SVF). MSCs were characterized using RT-PCR. To ascertain the trilineage differentiation potential, MSCs were stained with respective stain for osteocytes, chondrocytes and adipocytes. Result: We observed elongated, spindle-shaped and fibroblast like appearance of cells after 72 h of initial culture. Expression of MSC specific surface markers were observed through RT-PCR. Using Stem Pro® differentiation medium, OSE were differentiated into osteogenic, chondrogenic and adipogenic lineages and were found to be potential source for isolation, characterization and differentiation of MSCs. Canine (OSE) is easily accessible, multipotent and has high plasticity, holding promise for applications in regenerative medicine.

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