scholarly journals Comparative toxicity of abamectin and nano-derived form on land snail, Helix aspersa in attributing to cytotoxicity and biochemical alterations

2021 ◽  
Vol 10 (1) ◽  
pp. 296-311
Author(s):  
Khaled Yassin Abdel-Halim ◽  
Safaa Ramadan Osman ◽  
Heba Mohamed El-Danasoury ◽  
Gihan Fathy Aly
Keyword(s):  
Digestive Gland ◽  
Land Snail ◽  
Helix Aspersa ◽  
Land Snails ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Biochemical Alterations ◽  
Nano Emulsion ◽  
Snail Helix Aspersa

The comparative toxic effect of Vertimec® 1.8% EC, Fast Max Super® 8.4% SC and nano-derived form of abamectin (ABM) (1% nano-emulsion) as a dermal contact for 48 h against land snail, Helix aspersa was evaluated at laboratorial trail. Acute toxicity values (LD50) were 6.45, 11.97, and 45.95 µg snail-1 for nano-derived form of ABM, Fast Max Super® and Vertimec®, respectively. Nano-derived form exhibited the highest toxic effects (1.86 and 7.12-folds), respect to Fast Max Super® and Vertimec®. Sublethal doses: 1/10 and 1/100 LD50s of the examined compounds were applied to evaluate some biochemical alterations e.g. acetylcholinesterase (AChE), malondialdhyde (MDA), lactate dehydrogenase (LDH), glutathione-S-transferase (GST), acid phosphatase (ACP), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), respectively, in haemolymph and digestive glands homogenates. The all treatments significantly decreased AChE activity in ganglia homogenate, respect to control group (untreated). All treatments exhibited MDA level and LDH activity greater than the control in both haemolymph and digestive gland. This concept recognizes the cytotoxic effect of ABM on gastropods. Significant declines in GST, ACP, and ALP activities were exhibited in homogenate of digestive gland for the all treatments. However, AST/ALT activities exhibited increase greater than untreated group. These findings may explain the role of these doses of ABM for dysfunction in organs of H. aspersa. Thus, prepared nano-emulsion was more potent toxic on land snails. However, H. aspersa is considered a useful tool to assess ecotoxicological impact of pesticides.

Role of nociceptin/orphanin FQ and the pseudopeptide [Phe1Ψ(CH2NH)Gly2]-nociceptin(1–13)-NH2 and their interaction with classic opioids in the modulation of thermonociception in the land snail Helix aspersa

2008 ◽  
Vol 581 (1-2) ◽  
pp. 77-85 ◽  
Author(s):  
Carolina Miller-Pérez ◽  
Eduardo Sánchez-Islas ◽  
Francisco Pellicer ◽  
Gabriela Rodríguez-Manzo ◽  
Silvia L. Cruz ◽  
...  
Keyword(s):  
Land Snail ◽  
Helix Aspersa ◽  
Orphanin Fq ◽  
Snail Helix Aspersa

scholarly journals Acute effects of tartrazine (E102) exposure on behavior, and histology of land snail Helix aspersa biological model

2020 ◽  
pp. 43-51
Author(s):  
Smina AÏT HAMLET ◽  
Samira BENSOLTANE ◽  
Houria BERREBBAH
Keyword(s):  
Land Snail ◽  
Helix Aspersa ◽  
Biological Model ◽  
Control Group ◽  
Inhibitory Effects ◽  
Histological Changes ◽  
Biomarkers Of Exposure ◽  
Tissue Alterations ◽  
Snail Helix Aspersa ◽  
Experimental Group

Introduction. Tartrazine is an azofood additive widely used in the pharmaceu-cal, cosmetic, food, and other industries. Objective. To assess the effects of tartrazineon the attitude to move and to eat, and histological changes in digestive, and renal sys-tem, after an acute treatment on a biological model the snail Helix aspersa. Material and Methods. Snails were exposed orally, and by contact to flour contaminated with this dye for 24 hours. Adult snails were divided into two groups of 20 animals each. The control group fed flour alone. The experimental group was administered orally with flour treated with tartrazine (200mg/g flour). Results. The observation of behaviour revealed inhibitory effects of tartrazine on locomotion, and nutrition of snails. Histolo-gical examination of intestines, hepatopancreas, and kidney of treated animals showed altered tissue structures, with advanced degeneration of digestive tubules, connective tissue, and smooth muscle fibers of the walls surrounding the three organs, in response to tartrazine, compared to controls. Conclusion. This study suggests that acute exposure to tartrazine may lead to disorders in locomotor, and nutritional behavior, in addition to tissue alterations. Therefore, this study on behavior, supported by histological changes in H. Aspersa can be used as potential biomarkers of exposure to other azodyes.

The nociceptin/orphanin FQ-like opioid peptide in nervous periesophageal ganglia of land snail Helix aspersa

2013 ◽  
Vol 1505 ◽  
pp. 22-46 ◽  
Author(s):  
Martha León-Olea ◽  
Carolina Miller-Pérez ◽  
Eduardo Sánchez-Islas ◽  
José Mendoza-Sotelo ◽  
René Garduño-Gutiérrez ◽  
...  
Keyword(s):  
Land Snail ◽  
Opioid Peptide ◽  
Helix Aspersa ◽  
Orphanin Fq ◽  
Snail Helix Aspersa

Antioxidant defenses and metabolic depression in a pulmonate land snail

1995 ◽  
Vol 268 (6) ◽  
pp. R1386-R1393 ◽  
Author(s):  
M. Hermes-Lima ◽  
K. B. Storey
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Thiobarbituric Acid ◽  
Land Snail ◽  
Land Snails ◽  
Antioxidant Defenses ◽  
Glutathione S Transferase ◽  
Hypometabolic State

During arousal from estivation oxygen consumption by land snails (Otala lactea) increases severalfold. To determine whether snails prepared for an accompanying rise in the rates of oxyradical generation by altering their antioxidant defense mechanisms, changes in the activities of antioxidant enzymes and lipid peroxidation products were quantified in foot and hepatopancreas of control, 30-day estivating, and aroused snails. Compared with controls, estivating O. lactea showed significant increases in the activities of foot muscle superoxide dismutase (SOD) (increasing by 56-67%), catalase (51-72%), and glutathione S-transferase (79-108%), whereas, in hepatopancreas, SOD (57-78%) and glutathione peroxidase (93-144%) increased. Within 40 min after arousal began, hepatopancreas glutathione peroxidase activity had returned to control values, but SOD showed a further 70% increase in activity but then returned to control levels by 80 min. Estivation had no effect on total glutathione (GSH + 2 GSSG) concentrations in tissues, but GSSG content had increased about twofold in both organs of 30-day dormant snails. Lipid peoxidation (quantified as thiobarbituric acid reactive substances) was significantly enhanced at the onset of arousal from dormancy, indicating that oxidative stress and tissue damage occurred at this time. The data suggest that antioxidant defenses in snail organs are increased while snails are in the hypometabolic state as a preparation for oxidative stress during arousal.

Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa

2005 ◽  
Vol 39 (12) ◽  
pp. 1620-1628 ◽  
Author(s):  
F REGOLI ◽  
S GORBI ◽  
N MACHELLA ◽  
S TEDESCO ◽  
M BENEDETTI ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Land Snail ◽  
Helix Aspersa ◽  
Extremely Low Frequency ◽  
Snail Helix Aspersa
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