Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench

2019 ◽  
Vol 45 (6) ◽  
pp. 503-506
Author(s):  
E. N. Barsukova ◽  
A. G. Klykov ◽  
E. L. Chaikina
Author(s):  
E. N. Barsukova ◽  
A. G. Klykov ◽  
E. L. Chikina

The article presents results of usage of the tissue culture method on buckwheat (Fagopyrum esculentum Moench) together with the selective factor increased concentrations of heavy metal ions in the nutrient medium. On the basis of Izumrud (Russia), Kitavase (Japan), hybrid Izumrud x Kitavase there were developed regenerant lines tolerant to copper and zinc. Negative action effect of copper ions was observed from the second generation in the second posterity of the plants-regenerants, developed from the callus, in the form of the chlorophyll-free mutants. Positive effect of the heavy metals was observed on increasing of rutin content of the plants-regenerants in vitro. Selective evaluation in the field conditions of the samples tolerant to heavy metal let to choose from them the most adaptive to monsoon conditions in Primorskykrai, which combine valuable traits of big grain (weight of 1000 grains 36-38 g), high seed productivity (1,9-3,2 g) and high rutin content in aboveground mass of plants (2,8-4,3 %).


Microbiology ◽  
1982 ◽  
Vol 128 (11) ◽  
pp. 2817-2820
Author(s):  
J. A. Levy ◽  
P. E. Sumner ◽  
L. E. Hooser

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.


1990 ◽  
Vol 98 (1136) ◽  
pp. 404-407 ◽  
Author(s):  
Motohiro TORIYAMA ◽  
Sukezo KAWAMURA ◽  
Yukari KAWAMOTO ◽  
Takahiro SUZUKI ◽  
Yoshiyuki YOKOGAWA ◽  
...  

2008 ◽  
Vol 20 (2) ◽  
pp. 215-220
Author(s):  
JIANG Jinhui ◽  
◽  
ZHOU Changfang ◽  
AN Shuqing ◽  
GUAN Baohua ◽  
...  

1935 ◽  
Vol 31 (2) ◽  
pp. 271-272
Author(s):  
G. Barg

The author has shown in a series of experiments that the tissue culture method is quite applicable for the study of anaerobic bacteria and their toxins, especially those of them that are significantly weakened by filtration.


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