scholarly journals Development and Validation of Visible Spectrophotometric Methods for the Analysis of Etravirine: Application to Tablet Dosage Forms

2020 ◽  
Vol 4 (7) ◽  
pp. 03-11
Author(s):  
Murali Dadi ◽  
Indra Sen Singh ◽  
G Purna Chandra Rao
Keyword(s):  
Dosage Forms ◽  
Spectrophotometric Methods ◽  
Development And Validation ◽  
Tablet Dosage

scholarly journals Development and Validation of Spectrophotometric Methods for the Estimation of Mesalamine in Tablet Dosage Forms

2010 ◽  
Vol 2 (3) ◽  
pp. 284-288 ◽  
Author(s):  
K.M. Patel ◽  
C.N. Patel ◽  
B. Panigrahi ◽  
A.S. Parikh ◽  
H.N. Patel
Keyword(s):  
Dosage Forms ◽  
Spectrophotometric Methods ◽  
Development And Validation ◽  
Tablet Dosage

scholarly journals Development and Validation of Spectrophotometric Methods for the Quantification of Solifenacin Succinate: Application to Tablet Dosage Forms

2013 ◽  
Vol 15 ◽  
pp. 167-182
Author(s):  
Burla Sunitha Venkata Seshamamba ◽  
Peruri Veera Venkata Satyanarayana ◽  
Chandra Bala Sekaran
Keyword(s):  
Ammonium Thiocyanate ◽  
Dosage Forms ◽  
Ion Pair ◽  
Ammonium Molybdate ◽  
Ternary Complexes ◽  
Bromocresol Green ◽  
Spectrophotometric Methods ◽  
Solifenacin Succinate ◽  
Development And Validation ◽  
Tablet Dosage

Four sensitive, precise and accurate spectrophotometric methods for the estimation of solifenacin succinate (SFS) have been developed. Method A describes the interaction of SFS with potassium permanganate in alkaline medium to give green colored manganate ion with absorption maxima at 610 nm. Methods B and C are based on the formation of ternary complexes of SFS with, copper (II)/eosin (method B) and ammonium molybdate/ammonium thiocyanate (method C), respectively which are extracted into chloroform and have absorption maxima at 545 nm (method B) and 465 nm (method C). Method D was based on the formation of yellow colored ion-pair complex between bromocresol green and SFS in dichloromethane medium with absorption maxima at 415 nm. Regression analysis of Beer's law plot showed good correlation in the concentration range of 5-50, 2.5-50, 10-100 and 2-20 μg/mL for methods A, B, C and D, respectively. Different variables affecting the reaction were studied and optimized. The proposed methods were applied successfully for the analysis of SFS in tablets dosage forms. No interference was observed from common pharmaceutical excipients.

scholarly journals The development and validation of visible spectrophotometric methods for simvastatin determination in pure and the tablet dosage forms

2008 ◽  
Vol 14 (3) ◽  
pp. 205-210 ◽  
Author(s):  
Kanakapura Basavaiah ◽  
Kalsang Tharpa
Keyword(s):  
Acid Medium ◽  
Standard Addition ◽  
Dosage Forms ◽  
Spectrophotometric Methods ◽  
Red Color ◽  
Assay Precision ◽  
Development And Validation ◽  
Tablet Dosage

Two simple and sensitive spectrophotometric methods have been developed for the determination of simvastatin (SMT) in pure form and in tablets using insitu generated bromine, and p-phenylenediamine (PPDA) or o-dianisidine (ODA) as reagents. The methods are based on the bromination of SMT by a measured excess of in situ bromine in acid medium followed by the determination of unreacted bromine by reacting with PPDA and measuring the resulting red color at 510 nm (method A) or reacting with ODA and measuring the absorbance at 470 nm (method B). The conditions for the assay have been optimized. Beer's law is obeyed over the concentration ranges 20-120 and 2-12 ?g/ml for method A and method B, respectively. The calculated molar absorbtivities are 2.24?103 and 1.91?104 dm3 mol-1 cm-1 for the method A and the method B, respectively; 0.1868 and 0.0115 ?g/cm2 being the corresponding Sandell sensitivities. The LOD and LOQ for method A are found to be 2.96 and 8.97 ?g/ml, and the respective values for method B are 0.14 and 0.42 ?g/ml. The intra-day and inter-day precision and accuracies were checked. The assay precision was less than 5 % CV and the accuracy was 97.38-103.4 %. The methods were used for the determination of SMT in tablets. No interference from the excipients added to tablets was found. The accuracy and validity of the methods were further ascertained by recovery studies via the standard addition technique.

Method Development and Validation of Spectrophotometric Methods for The Estimation of Rizatriptan Benzoate in Pure and Pharmaceutical Dosage Form

2021 ◽  
pp. 6003-6006
Author(s):  
Pushpa Latha E. ◽  
Sailaja B.
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Form ◽  
Rizatriptan Benzoate ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

Analytical UV derivative spectrophotometric method was developed and validated to quantify Rizatriptan Benzoate in pure drug and tablet dosage form. Based on the spectrophotometric characteristics of Rizatriptan Benzoate, a signal of zero (225nm), first (216nm), second (237nm), third (233nm), fourth (231nm) order derivative spectra were found to be adequate for quantification. The methods obeyed Beer's law in the concentration range of (0.1-360µg/ml) with square correlation coefficient (r2) of 0.999. The mean percentage recovery was found to be 100.01 ± 0.075. As per ICH guidelines the results of the analysis were validated in terms of linearity, precision, accuracy, limit of detection and limit of quantification, and were found to be satisfactory.

scholarly journals Uv Spectrophotometric Methods for Determination of Sofosbuvir in Pure Form and Pharmaceutical Dosage Forms in Presence of Its Alkaline Degradate

2020 ◽  
pp. 12-25
Author(s):  
Zeinab Adel Nasr ◽  
Noha S. Said ◽  
Sawsan A. Abdel-Razeq
Keyword(s):  
Good Accuracy ◽  
Dosage Forms ◽  
Difference Spectra ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods ◽  
Good Linearity ◽  
Ratio Difference ◽  
Accuracy And Precision ◽  
Tablet Dosage

Aims: Two spectrophotometric methods were developed and validated for the determination of sofosbuvir in presence of its alkaline degradate. Study Design: Ratio difference and ratio derivative methods were assisted for determination of sofosbuvir in presence of its alkaline degradate, laboratory-prepared mixtures and in tablet dosage forms. Place and Duration of Study: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy (Girls), Al - Azhar University, between December 2019 and January 2020. Methodology: Two analytical methods were achieved and validated for the quantitative determination of Sofosbuvir in presence of its alkaline degradate. The first method was ratio difference (RD) method, where the UV absorption spectra of different concentrations of sofosbuvir were divided by the spectrum of a certain concentration (15 µg mL-1) as a devisor of its alkaline degradate to get the ratio difference spectra. Afterwards, the peak amplitudes difference between 253.7 and 243.5 nm were measured. The second method was the ratio derivative (1DR) method, where the first derivative of the ratio spectra (1DR) was obtained and its amplitude was measured at 247 and 268 nm. Good linearity was obtained over the concentration range of 3-15 µg mL-1 for the proposed methods. The proposed procedures were adopted for the selective determination of intact Sofosbuvir in presence of up to 80% of its degradation product. Sofosbuvir was exposed to different conditions as alkaline, acidic and oxidative degradation. Results: The proposed methods were developed and validated with good linearity range of 3-15 µg mL-1 for both methods, and also with good accuracy and precision. And the obtained results were statistically compared to those obtained by the reported method. Conclusion: Sofosbuvir was successfully determined by the proposed ratio difference and ratio derivative methods in bulk powder, laboratory prepared mixtures and tablet dosage form with good accuracy and precision. The methods were validated according to ICH guidelines. The results obtained were compared with those of the reported method and were found to be in good agreement.

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