Simultaneous, quantitative measurement of local blood flow and glucose utilization in tissue samples in normal and injured feline brain

Brain Injury ◽  
1988 ◽  
Vol 2 (4) ◽  
pp. 291-303 ◽  
Author(s):  
Douglas S. Dewitt ◽  
Xiao-Quan Yuan ◽  
Donald P. Becker ◽  
Ronald L. Hayes
Keyword(s):  
Blood Flow ◽  
Quantitative Measurement ◽  
Glucose Utilization ◽  
Local Blood Flow ◽  
Tissue Samples

Quantitative measurement of local blood flow with heat clearance

1975 ◽  
Vol 70 (5) ◽  
pp. 547-567 ◽  
Author(s):  
W. Müller-Schauenburg ◽  
H. Apfel ◽  
H. Benzing ◽  
E. Betz
Keyword(s):  
Blood Flow ◽  
Quantitative Measurement ◽  
Local Blood Flow ◽  
Heat Clearance

scholarly journals Effect of Apomorphine on the Relationship between Local Cerebral Glucose Utilization and Local Cerebral Blood Flow (with an Appendix on its Statistical Analysis)

1982 ◽  
Vol 2 (4) ◽  
pp. 487-499 ◽  
Author(s):  
James McCulloch ◽  
Paul A. T. Kelly ◽  
Ian Ford
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Anterior Cingulate ◽  
Cerebellar Hemisphere ◽  
Tissue Blood Flow ◽  
Local Blood Flow ◽  
Local Cerebral Blood Flow ◽  
Major Mechanism ◽  
The Relationship

The alterations in local glucose utilization and local blood flow in 36 discrete regions of the central nervous system (CNS) that occur following the intravenous administration of the putative dopaminergic agonist, apomorphine (0.5 mg/kg), have been measured using the quantitative autoradiographic 14C-2-deoxyglucose and 14C-iodoantipyrine techniques. In eight of the regions examined (frontal and sensory-motor cortices, ventral thalamus, caudate nucleus, globus pallidus, substantia nigra, subthalamic nucleus, and posterior cerebellar hemisphere), significant elevations of local cerebral blood flow (CBF) were observed following apomorphine administration. In these eight regions, proportionately similar, significant elevations in local glucose utilization were observed following apomorphine. In two of the regions investigated (anterior cingulate cortex and lateral habenular nucleus), significant reductions in both local blood flow and glucose utilization were observed following apomorphine administration. In the majority of regions examined (26 of the total 36), apomorphine did not alter significantly either blood flow or glucose use. Using a statistical approach, described in detail in an appendix, the relationship between local rates of glucose utilization and local levels of tissue blood flow was analyzed. A relationship between local CBF and local glucose utilization was found following apomorphine, and the nature of this relationship was indistinguishable from that observed in control animals. In no region of the CNS was a significant deviation from the normal CBF–glucose use relationship demonstrated following apomorphine administration. These results point to the greater importance of the effects of apomorphine upon tissue metabolic activity, rather than its direct vascular action, as being the major mechanism underlying the observed alterations in local CBF. The statistical methods provide a rigorous analytical approach to the analysis of alterations in the relationship, both locally and globally, of blood supply to glucose utilization.

scholarly journals Transient Middle Cerebral Artery Occlusion by Intraluminal Suture: II. Neurological Deficits, and Pixel-Based Correlation of Histopathology with Local Blood Flow and Glucose Utilization

1997 ◽  
Vol 17 (12) ◽  
pp. 1281-1290 ◽  
Author(s):  
Weizhao Zhao ◽  
Ludmila Belayev ◽  
Myron D. Ginsberg
Keyword(s):  
Blood Flow ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Glucose Utilization ◽  
Artery Occlusion ◽  
Data Sets ◽  
Local Blood Flow ◽  
Postural Reflex ◽  
Cerebral Artery Occlusion

We conducted a pixel-based analysis of the acute hemodynamic and metabolic determinants of infarctive histopathology in a reproducible model of temporary (2-hour) middle cerebral artery occlusion (MCAO) produced in rats by an intraluminal suture. Three-dimensional averaged image data sets of local cerebral blood flow (LCBF) and glucose utilization (LCMRglc) acquired in the companion study ( Belayev et al., 1997 ) either at the end of a 2-hour period of MCAO or after 1 hour of recirculation were comapped (using digitized atlas-templates) with data sets depicting the frequency of histological infarction in a matched animal group (n = 8) in which 2 hours of MCAO was followed by 3-day survival, sequential neurobehavioral examinations, and perfusion-fixation and paraffin-embedding of brains for light-microscopic analysis. All rats developed marked postural-reflex and forelimb-placing deficits at 60 minutes of MCAO, signifying high-grade ischemia. Tactile placing deficits persisted during the 72-hour observation period while visual placing and postural-reflex abnormalities variably improved. Comapping of LCBF and histopathology showed that in those pixels destined to undergo infarction, LCBF measured at 2 hours of MCAO showed a sharp distributional peak centered at 0.14 mL/g/min. In 70% of pixels destined to infarct, LCBF at 2 hours of MCAO was 0.24 mL/g/min or below, and in 89% LCBF was below 0.47 mL/g/min (the upper limits of the ischemic core and penumbra, respectively, as defined in the companion study [ Belayev et al., 1997 ]). Local cerebral glucose utilization measured at ~1 hour after 2 hours of MCAO was distributed bimodally in the previously ischemic hemisphere. The major peak, at 22 μmol/100 g/min, coincided exactly with the distribution peak of pixels destined to undergo infarction, while in pixels with a zero probability of infarction, LCMRglc was higher by 12 to 13 μmol/100 g/min. These results indicate that local blood flow at 2 hours of MCAO is a robust predictor of eventual infarction. Pixels with ischemic-core levels of LCBF (0% to 20% of control) have a 96% probability of infarction, while the fate of the penumbra is more heterogeneous: below LCBF of 0.35 mL/g/min, the probability of infarction is 92%, while approximately 20% pixels in the upper-penumbral LCBF range (30% to 40% of control) escape infarction. Our data strongly support the view that the likelihood of infarction within the ischemic penumbra is highly influenced by very subtle differences in early perfusion.

Blood flow and high-energy phosphates in microregions of left ventricular subendocardium

1981 ◽  
Vol 240 (5) ◽  
pp. H804-H810 ◽  
Author(s):  
H. D. Kleinert ◽  
H. R. Weiss
Keyword(s):  
Blood Flow ◽  
Linear Relationship ◽  
Tissue Perfusion ◽  
High Energy ◽  
Left Ventricular ◽  
Significant Loss ◽  
Tissue Blood Flow ◽  
High Energy Phosphates ◽  
Tissue Samples ◽  
Heterogeneous Distribution

Blood flow and high-energy phosphate (HEP) content were determined simultaneously in multiple microregions of left ventricular subendocardium in 29 normal anesthetized open-chest rabbits by use of a new micromethod to determine whether a direct linear relationship existed between these parameters. Tissue samples weighed 1-2 mg. ATP and creatine phosphate (CP) content were quantitated in quick-frozen hearts by fluorometry at sites where tissue perfusion was measured by H2 clearance by use of bare-tipped platinum electrodes. A series of validation studies were conducted to ensure that 1) no significant damage to the tissue surrounding the electrode occurred during the period of experimentation and 2) no significant loss of biochemical constituents had occurred due to labile processes during freezing or storage of the tissue. Blood flow, ATP, and CP values averaged 79.1 +/- 24.1 (SD) ml.min-1.100 g-1, 4.9 +/- 1.3 mumol/g tissue, and 8.0 +/- 3.0 mumol/g tissue, respectively, and are similar to those reported in studies using larger tissue samples. Correlation between the heterogeneous distribution of tissue perfusion and HEP revealed no direct linear relationship between these parameters in the normal unstressed rabbit subendocardium.

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