In vitro production of basement membrane collagen by a clonal line of mouse lens epithelial cells

1981 ◽  
Vol 1 (5) ◽  
pp. 267-273 ◽  
Author(s):  
David H. Rohrbach ◽  
Paul Russell ◽  
Robert L. Church
1978 ◽  
Vol 115 (1) ◽  
pp. 127-142 ◽  
Author(s):  
Maryvonne Laurent ◽  
Marie-Odile Lonchampt ◽  
François Regnault ◽  
Jacqueline Tassin ◽  
Yves Courtois

1976 ◽  
Vol 251 (3) ◽  
pp. 730-733 ◽  
Author(s):  
N A Kefalides ◽  
J D Cameron ◽  
E A Tomichek ◽  
M Yanoff

Cell ◽  
1980 ◽  
Vol 22 (3) ◽  
pp. 719-726 ◽  
Author(s):  
Victor P. Terranova ◽  
David H. Rohrbach ◽  
George R. Martin

Development ◽  
1987 ◽  
Vol 99 (1) ◽  
pp. 25-32
Author(s):  
A.L. Muggleton-Harris ◽  
N. Higbee

The morphological and cellular changes that occur with differentiation and development of a lentoid structure from cultured mouse lens epithelial cells have been found to be dependent on the presence of lens capsule in association with the cells. The development of the ‘lentoid body’ is a multiphase process involving cell replication, synthesis of mucosubstances and a basement collagen membrane, cell aggregation and differentiation. Stage-specific synthesis of lens proteins confirms that the genes regulating normal differentiation in vivo are operating in the in vitro system. The hydrated collagen gel studies described in this report demonstrate that the cuboidal morphology and apical-basal polarity of the lens epithelial cells are dependent on their relationship with the lens capsule. Following a replicative phase the cells assume a mesenchyme-like morphology and migrate into the gel. Trypsinized cells freed from the lens capsule replicate but form colonies on the surface of the gel. The implications of these results are discussed with respect to previous observations made on normal lens development and the abnormalities associated with the congenital cataractous embryonic lens.


2020 ◽  
Vol 142 ◽  
pp. 103179
Author(s):  
Thanapol Nongbua ◽  
Yongzhi Guo ◽  
Theodoros Ntallaris ◽  
Marie Rubér ◽  
Heriberto Rodriguez-Martinez ◽  
...  

Author(s):  
Martin Clynes ◽  
Margaret Dooley ◽  
Breda Carey ◽  
Anne Godfrev ◽  
Angela O'Toole ◽  
...  

1980 ◽  
Vol 9 (4) ◽  
pp. 359-366 ◽  
Author(s):  
S. Garbisa ◽  
K. Kniska ◽  
K. Tryggvason ◽  
C. Foltz ◽  
L.A. Liotta

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