Laser Doppler Flowmetry and Transcutaneously Measured Carbon Dioxide Tension for Observing Changes of Skin Blood Flow in Fingers

Introduction. Neuroretinoprotective activity of melatonin is the basis for further comprehensive assessment of possible mechanisms of its protective action in order to reasonable implementation of the drug in ophthalmic practice as a remedy with neuroretinoprotective action for the treatment of traumatic lesions of eye.The aim of the study – to learn Laser Doppler Flowmetry method to investigate the effect of melatonin and reference drugs (citicoline, corvitin and thiotriazoline) on completeness of recovery of blood flow in the vessels of the ciliary body in dynamic of its contusion as a possible mechanism of their neuroretinoprotective action.Research Methods. Model of eye contusion is a blank shot into the center of the cornea closely with carbon dioxide under pressure. Therapy is separate intravenous administration of drugs (melatonin 10 mg / kg, corvitin 10 mg / kg citicoline 250 mg / kg and thiotriazoline 100 mg / kg) twice a day during 7 days, the first application 1 h after injury. Effect of drugs on microcirculation in the eye ciliary body in this condition was studied with using Laser Doppler Flowmetry module BIOPAC (USA).Results and Discussion. Therapeutic application of all test substances amortized rapid deterioration of blood supply to the eye. Melatonin is the leader among the selected drugs for the ability to improve the circulation in the ciliary body of eye during the first week after its contusion.Conclusion. Restoration of eye perfusion caused by contusion on the background of application of melatonin, citicoline, corvitin and thiotriazoline is one of the leading mechanisms of neuroretinoprotective action of these drugs.

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Evaluation of skin blood flow by laser Doppler flowmetry

Clinics in Dermatology ◽

10.1016/0738-081x(95)00080-y ◽

1995 ◽

Vol 13 (4) ◽

pp. 337-347 ◽

Cited By ~ 31

Author(s):

Hee Chul Eun

Keyword(s):

Blood Flow ◽

Laser Doppler Flowmetry ◽

Skin Blood Flow ◽

Laser Doppler ◽

Doppler Flowmetry

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Cutaneous blood flow in the pigeon Columba livia: its possible relevance to cutaneous water evaporation

Journal of Experimental Biology ◽

10.1242/jeb.205.17.2627 ◽

2002 ◽

Vol 205 (17) ◽

pp. 2627-2636 ◽

Cited By ~ 3

Author(s):

E. Ophir ◽

Y. Arieli ◽

J. Marder ◽

M. Horowitz

Keyword(s):

Blood Flow ◽

Laser Doppler Flowmetry ◽

Skin Blood Flow ◽

Heat Exposure ◽

Columba Livia ◽

Laser Doppler ◽

Arterial Blood Flow ◽

Water Evaporation ◽

Venous Blood Flow ◽

Doppler Flowmetry

SUMMARY The heat-acclimated rock pigeon is thought to use cutaneous water evaporation (CWE) as the `preferred' route for heat dissipation, and this mechanism is controlled by adrenergic signaling. In the present study, we tested the hypothesis that adjustments in skin blood flow are a crucial component of this adaptation. Skin blood flow was measured by laser Doppler flowmetry and by ultrasonic flowmetry in heat-acclimated (HAc) and non-acclimated (NAc) pigeons. Skin blood flow, CWE and rectal and skin temperatures were measured under heat exposure(Ta=50°C) or following propranolol (1.3 mg kg-1) or clonidine (80 μg kg-1) administration. Using laser Doppler flowmetry, we found a significant increase (1.3-fold) in skin blood flow in the dorsal skin of HAc pigeons following propanolol administration. In contrast, a significant decrease (0.7-fold) was observed in NAc birds. Injection of clonidine resulted in a significant decrease in skin blood flow in both HAc and NAc pigeons (0.4- and 0.5-fold, respectively). Heat exposure increased blood perfusion in both groups (2.5- and 1.8-fold,respectively). Using ultrasonic flowmetry, we showed that both propanolol and clonidine increase the arterial blood flow (Qa) in HAc pigeons, while venous blood flow (Qv) decreases. In contrast, no significant changes were found in NAc pigeons. As shown by the effect of clonidine, augmentation of skin blood flow is not a prerequisite for CWE, but normally coincides with a greater difference in arterial—venous pressure. Possible regulatory mechanisms are discussed.

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