Measurement of Infrasound Components Contained in the Noise Emitted during a Working Wind Turbine

The research reported in this paper involves the development and refinement of methods applicable to the measurement and analysis of infrasound signals generated by the operation of wind turbines. In particular, the presentation focuses on the use of a new system that is applied for simultaneous recording of acoustic signals in the low-frequency range emitted by wind farms in three independent and identical measurement setups. A comparative analysis of the proposed new system was made with the Brüel & Kjaer measurement, a commonly used methodology, which meets the requirements of the IEC 61400-11 standard. The paper focuses on the results of frequency and time-frequency analysis of infrasound signals recorded throughout the operation of a wind turbine with a rated capacity of 2 MW. The use of a correlated system with three simultaneous measurement systems can be a new and alternative measurement method that will eliminate the drawbacks of previous approaches.

Early Schizophrenia and Bipolar Disorder Patients Display Reduced Neural Prepulse Inhibition

Background: Altered sensorimotor gating has been demonstrated by Prepulse Inhibition (PPI) tests in patients with psychosis. Recent advances in signal processing methods allow assessment of neural PPI through electroencephalogram (EEG) recording during acoustic startle response measures (classic muscular PPI). Simultaneous measurements of muscular (eye-blink) and neural gating phenomena during PPI test may help to better understand sensorial processing dysfunctions in psychosis. In this study, we aimed to assess simultaneously muscular and neural PPI in early bipolar disorder and schizophrenia patients. Method: Participants were recruited from a population-based case-control study of first episode psychosis. PPI was measured using electromyography (EMG) and EEG in pulse alone and prepulse + pulse with intervals of 30, 60, and 120 ms in early bipolar disorder (n = 18) and schizophrenia (n = 11) patients. As control group, 15 socio-economically matched healthy subjects were recruited. All subjects were evaluated with Rating Scale, Hamilton Rating Scale for Depression, and Young Mania Rating Scale questionnaires at recruitment and just before PPI test. Wilcoxon ranked sum tests were used to compare PPI test results between groups. Results: In comparison to healthy participants, neural PPI was significantly reduced in PPI 30 and PPI60 among bipolar and schizophrenia patients, while muscular PPI was reduced in PPI60 and PPI120 intervals only among patients with schizophrenia. Conclusion: The combination of muscular and neural PPI evaluations suggested distinct impairment patterns among schizophrenia and bipolar disorder patients. Simultaneous recording may contribute with novel information in sensory gating investigations.

The role of action potential changes in depolarization-induced failure of excitation contraction coupling in mouse skeletal muscle

Excitation-contraction coupling (ECC) is the process by which electrical excitation of muscle is converted into force generation. Depolarization of skeletal muscle resting potential contributes to failure of ECC in diseases such as periodic paralysis, intensive care unit acquired weakness and possibly fatigue of muscle during vigorous exercise. When extracellular K+ is raised to depolarize the resting potential, failure of ECC occurs suddenly, over a narrow range of resting potentials. Simultaneous imaging of Ca2+ transients and recording of action potentials (APs) demonstrated failure to generate Ca2+ transients when APs peaked at potentials more negative than –30mV. An AP property that closely correlated with failure of the Ca2+ transient was the integral of AP voltage with respect to time. Simultaneous recording of Ca2+ transients and APs with electrodes separated by 1.6mm revealed AP conduction fails when APs peak below –21mV. We hypothesize propagation of APs and generation of Ca2+ transients are governed by distinct AP properties: AP conduction is governed by AP peak, whereas Ca2+ release from the sarcoplasmic reticulum is governed by AP integral. The reason distinct AP properties may govern distinct steps of ECC is the kinetics of the ion channels involved. Na channels, which govern propagation, have rapid kinetics and are insensitive to AP width (and thus AP integral) whereas Ca2+ release is governed by gating charge movement of Cav1.1 channels, which have slower kinetics such that Ca2+ release is sensitive to AP integral. The quantitative relationships established between resting potential, AP properties, AP conduction and Ca2+ transients provide the foundation for future studies of failure of ECC induced by depolarization of the resting potential.

The Complex of Experimental Facilities for the Cosmic Ray Investigation at the Tien Shan Mountain Station

The study describes the experimental complex of the station located in the Tien Shan mountains at an elevation of 3340 m above sea level. The complex consists of detectors of different types scattered across the station area, such as scintillation particles detectors, Cherenkov detectors, radio emission detectors for the measurement of the electron component of extensive air showers (EAS) created by the (1–1000) PeV cosmic ray particles, an ionization calorimeter and neutron detectors for the study of the nuclear-active component of EAS cores, and the underground particle detectors for the detection of cosmic ray muons. The data acquisition system allows the simultaneous recording of parameters from various stand-alone detectors registering an EAS, and storage of the acquired data in the database. As an illustration of research capability, the results of the EAS study are presented here which were obtained during the last few years at the different experimental set-ups constituting the Tien Shan complex.

Automatic Clustering of Students by Level of Situational Interest Based on Their EEG Features

The usage of physiological measures in detecting student’s interest is often said to improve the weakness of psychological measures by decreasing the susceptibility of subjective bias. The existing methods, especially EEG-based, use classification, which needs a predefined class and complex computational to analyze. However, the predefined classes are mostly based on subjective measurement (e.g., questionnaires). This work proposed a new scheme to automatically cluster the students by the level of situational interest (SI) during learning-based lessons on their electroencephalography (EEG) features. The formed clusters are then used as ground truth for classification purposes. A simultaneous recording of EEG was performed on 30 students while attending a lecture in a real classroom. The frontal mean delta and alpha power as well as the frontal alpha asymmetry metric served as the input for k-means and Density-Based Spatial Clustering of Applications with Noise (DBSCAN) clustering algorithms. Using the collected data, 29 models were trained within nine domain classifiers, then the classifiers with the highest performance were selected. We validated all the models through 10-fold cross-validation. The high SI group was clustered to students having lower frontal mean delta and alpha power together with negative Frontal Alpha Asymmetry (FAA). It was found that k-means performed better by giving the maximum performance assessment parameters of 100% in clustering the students into three groups: high SI, medium SI and low SI. The findings show that the DBSCAN had reduced the performance to cluster dataset without the outlier. The findings of this study give a promising option to cluster the students by their SI level, as well as address the drawbacks of the existing methods, which use subjective measures.

Spontaneous Neuronal Oscillations in the Human Insula are Hierarchically Organized Traveling Waves

The insula plays a fundamental role in a wide range of adaptive human behaviors, but its electrophysiological dynamics are poorly understood. Here we used human intracranial electroencephalographic recordings to investigate the electrophysiological properties and hierarchical organization of spontaneous neuronal oscillations within the insula. We analyzed the neuronal oscillations of the insula directly and found that rhythms in the theta and beta frequency oscillations are widespread and spontaneously present. These oscillations are largely organized along the anterior-posterior axis of the insula. Both the left and right insula showed anterior-to-posterior decreasing gradients for the power of oscillations in the beta frequency band. The left insula also showed a posterior-to-anterior decreasing frequency gradient and an anterior-to-posterior decreasing power gradient in the theta frequency band. In addition to measuring the power of these oscillations, we also examined the phase of these signals across simultaneous recording channels and found that the insula oscillations in the theta and beta bands are traveling waves. The strength of the traveling waves in each frequency was positively correlated with the amplitude of each oscillation. However, the theta and beta traveling waves were uncoupled to each other in terms of phase and amplitude, which suggested that insula traveling waves in the theta and beta bands operate independently. Our findings provide new insights into the spatiotemporal dynamics and hierarchical organization of neuronal oscillations within the insula, which, given its rich connectivity with widespread cortical regions, indicates that oscillations and traveling waves have an important role in intra- and inter-insula communication.

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (Vm) are invaluable tools to study cellular signaling pathways. Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing (FASTM). Different from present multiplexing approaches, FASTM uses phase-sensitive signal detection, which renders various combinations of common probes for Vm and ions accessible for multiplexing. Using kinetic stopped-flow fluorimetry, we show that FASTM allows simultaneous recording of rapid changes in Ca2+, pH, Na+, and Vm with high sensitivity and minimal crosstalk. FASTM is also suited for multiplexing using single-cell microscopy and genetically-encoded FRET biosensors. Moreover, FASTM is compatible with opto-chemical tools to study signaling using light. Finally, we show that the exceptional time resolution of FASTM also allows resolving rapid chemical reactions. Altogether, FASTM opens new opportunities for interrogating cellular signaling.

Correlation of Pao2/Fio2 Ratio with Spo2/Fio2 Ratio in Children on Mechanical Ventilation

Background: Mechanical ventilation is frequently used in pediatric patients to ensure adequate gas exchange, improve respiratory distress, and to resolve pulmonary or other disorders. Monitoring of various parameters which are invasive, while patient is on mechanical ventilation it is fundamental to observe the progress of patient’s condition. We conducted this study to observe if PaO2/FiO2 and the ratio of SPO2 /FiO2 can be used interchangeably, we can have option for lesser invasive parameter. Aim: To determine the correlation of PaO2/FiO2 with SPO2 /FiO2 ratio in children on mechanical ventilation. Methods: A Cross-sectional study, Non-probability and purposive sampling. The study conducted at The Children’s hospital and the Institute of Child Health, Lahore from 04-10-2017 to 03-06-2018. Informed consent was obtained. Arterial blood gas sampling for calculation of PaO2/FiO2 ratio and simultaneous recording of SPO2 for measurement of SPO2/FiO2 ratio was done. The data collected was analysed on S.P.S.S (Statistical Package for Social Sciences) version 22. Results: In this study 30 patients on mechanical ventilation were enrolled with mean age of 58.55±5 months. There was male predominance. (M: F ratio 2:1). This study showed a strong positive correlation of the PaO2/FiO2 with SPO2/FiO2 that is r=0.603. Conclusion: According to our study noninvasive SpO2/FiO2 ratio (PFr) can reliably be used in place of PaO2/FiO2 ratio(SFr) in children on mechanical ventilation as a strong correlation was observed between them. The advantage is invasive arterial sampling can be replaced by non-invasive pulse oximetry for oxygen saturation. Key words: Mechanical Ventilation, Pediatric ICU, SPO2/FiO2, PaO2/FiO2

Reflection of the Ictal Electrocorticographic Discharges Confined to the Medial Temporal Lobe to the Scalp-Recorded Electroencephalogram

Objective: Previous reports on the simultaneous recording of electroencephalography (EEG) and electrocorticography (ECoG) have demonstrated that, in patients with temporal lobe epilepsy (TLE), ictal ECoG discharges with an amplitude as high as 1000 μV originating from the medial temporal lobe could not be recorded on EEG. In contrast, ictal EEG discharges were recorded after ictal ECoG discharges propagated to the lateral temporal lobe. Here, we report a case of TLE in which the ictal EEG discharges, corresponding to ictal ECoG discharges confined to the medial temporal lobe, were recorded. Case report: In the present case, ictal EEG discharges were hardly recognized when the amplitude of the ECoG discharges was less than 1500 μV. During the evolution and burst suppression phase, corresponding to highly synchronized ECoG discharges with amplitudes greater than 1500 to 2000 μV, rhythmic negative waves with the same frequency were clearly recorded both on the lateral temporal lobe and scalp. The amplitude of the lateral temporal ECoG was approximately one-tenth of that of the medial temporal ECoG. The amplitude of the scalp EEG was approximately one-tenth of that of the lateral temporal ECoG. Conclusions: Highly synchronized ictal ECoG discharges with high amplitude of greater than 1500 to 2000 μV in the medial temporal lobe could be recorded on the scalp as ictal EEG discharges via volume conduction.