Subcellular Distribution of Basic Fibroblast Growth Factor in Human Hepatoma Cells

1991 ◽  
Vol 4 (3) ◽  
pp. 189-196 ◽  
Author(s):  
David R. Brigstock ◽  
Joachim Sasse ◽  
Michael Klagsbrun
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Subcellular Distribution ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Fibroblast Growth ◽  
Human Hepatoma Cells

scholarly journals Purification from a human hepatoma cell line of a basic fibroblast growth factor-like molecule that stimulates capillary endothelial cell plasminogen activator production, DNA synthesis, and migration.

1986 ◽  
Vol 6 (11) ◽  
pp. 4060-4066 ◽  
Author(s):  
M Presta ◽  
D Moscatelli ◽  
J Joseph-Silverstein ◽  
D B Rifkin
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Plasminogen Activator ◽  
Basic Fibroblast Growth Factor ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Fibroblast Growth ◽  
Human Hepatoma Cell ◽  
Capillary Endothelial Cells

A 17,500-dalton protein which stimulates plasminogen activator production in cultured bovine capillary endothelial cells has been purified from a SK-Hep-1 human hepatoma cell lysate by using heparin affinity chromatography and fast protein-liquid ion exchange chromatography. The purified molecule stimulated plasminogen activator production in a dose-dependent manner between 0.01 and 1 ng/ml. It also stimulated collagenase synthesis, DNA synthesis, and motility in capillary endothelial cells in the same concentration range. This molecule was identified as a basic fibroblast growth factor-like molecule on the basis of its biological activity, its affinity for heparin-Sepharose, and its cross-reactivity with a polyclonal antibody raised against the human placental basic fibroblast growth factor.

Purification from a human hepatoma cell line of a basic fibroblast growth factor-like molecule that stimulates capillary endothelial cell plasminogen activator production, DNA synthesis, and migration

1986 ◽  
Vol 6 (11) ◽  
pp. 4060-4066
Author(s):  
M Presta ◽  
D Moscatelli ◽  
J Joseph-Silverstein ◽  
D B Rifkin
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Plasminogen Activator ◽  
Basic Fibroblast Growth Factor ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Fibroblast Growth ◽  
Human Hepatoma Cell ◽  
Capillary Endothelial Cells

A 17,500-dalton protein which stimulates plasminogen activator production in cultured bovine capillary endothelial cells has been purified from a SK-Hep-1 human hepatoma cell lysate by using heparin affinity chromatography and fast protein-liquid ion exchange chromatography. The purified molecule stimulated plasminogen activator production in a dose-dependent manner between 0.01 and 1 ng/ml. It also stimulated collagenase synthesis, DNA synthesis, and motility in capillary endothelial cells in the same concentration range. This molecule was identified as a basic fibroblast growth factor-like molecule on the basis of its biological activity, its affinity for heparin-Sepharose, and its cross-reactivity with a polyclonal antibody raised against the human placental basic fibroblast growth factor.

A Case of Contact Dermatitis due to Fiblast Spray, a Product of Basic Fibroblast Growth Factor

2006 ◽  
Vol 68 (3) ◽  
pp. 248-250 ◽  
Author(s):  
Shuko OKADA ◽  
Takashi MASU ◽  
Takahiko TSUNODA ◽  
Ryuhei OKUYAMA ◽  
Setsuya AIBA
Keyword(s):  
Contact Dermatitis ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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