circTAF8 Regulates Myoblast Development and Associated Carcass Traits in Chicken

Recent studies have shown that circular RNAs (circRNAs) play important roles in skeletal muscle development. CircRNA biogenesis is dependent on the genetic context. Single-nucleotide polymorphisms in the introns flanking circRNAs may be intermediate-inducible factors between circRNA expression and phenotypic traits. Our previous study showed that circTAF8 is an abundantly and differentially expressed circRNA in leg muscle during chicken embryonic development. Here, we aimed to investigate circTAF8 function in muscle development and the association of the SNPs in the circTAF8 flanking introns with carcass traits. In this study, we observed that overexpression of circTAF8 could promote the proliferation of chicken primary myoblasts and inhibit their differentiation. In addition, the SNPs in the introns flanking the circTAF8 locus and those associated with chicken carcass traits were analyzed in 335 partridge chickens. A total of eight SNPs were found associated with carcass traits such as leg muscle weight, live weight, and half and full-bore weight. The association analysis results of haplotype combinations were consistent with the association analysis of a single SNP. These results suggest that circTAF8 plays a regulatory role in muscle development. These identified SNPs were found correlated with traits to muscle development and carcass muscle weight in chickens.

Morphological and Functional Characteristics of the Peroneus Muscles in Patients with Lateral Ankle Sprain: An Ultrasound-Based Study

Background and Objectives: The effectiveness of multiple ultrasound evaluations of the peroneus muscles morphology, including muscle cross-sectional area (CSA) and connective tissue, after lateral ankle sprain (LAS) is unknown. This study aimed to measure the peroneus muscles after LAS at three points, adding distal 75% to the conventional measurement points, in order to obtain a detailed understanding of the post-injury morphology and to propose a new evaluation index of the peroneus muscles for multiple LAS. Materials and Methods: Participants with and without LAS (LAS and control groups, 16 each) were recruited. The muscle cross-sectional area (CSA) and muscle echogenicity were measured using a B-mode ultrasound system at 25%, 50%, and 75% proximal to the line connecting the fibular head to the lateral malleolus. The ankle evertor strength was measured using a handheld dynamometer. Simultaneously, the peroneus longus (PL) and peroneus brevis (PB) muscle activities were measured using surface electromyography. Measurements for the LAS side, non-LAS side, and control leg were performed separately. Results: The CSA was significantly higher at 75% on the LAS side than on the non-LAS side and in the control leg. Muscle echogenicity of the LAS side at 75% was significantly lower than that of the non-LAS side and the control leg. Muscle activity of the PL was significantly lower and the PB was higher on the LAS side than on the non-LAS side and in the control leg. Conclusions: The PL was less active than the PB, while the PB was found to be overactive, suggesting that PB hypertrophy occurs due to an increase in the percentage of muscle fibers and a decrease in the connective tissue. Therefore, it is necessary to evaluate the condition of the PL and PB separately after LAS.

Survei Kondisi Fisik Sepak Bola pada Pemain Arema Football Academy Usia 16 Tahun di Kota Malang Tahun 2021

Physical condition is an important role in football because with good physical condition, it will be more likely to achieve. This study aims to determine the level of physical condition of Arema players aged 16 years in Malang City in 2021. The method used in this study is descriptive quantitative which aims to provide a real picture in accordance with what is in the field about the physical condition of Arema Football Academy football players aged 16 years old in Malang in 2021. The results on leg muscle strength have an average of 44.24, speed has an average of 7.73, agility has an average of 9.91, flexibility has an average of 16.60, muscle power limbs have an average of 55.88, endurance has an average of 45.98. It can be concluded that strength (leg muscles) is in sufficient category, speed is in good category, agility is in very good category, flexibility is in very good category, leg muscle power is in good category and endurance is in good category. And the overall physical condition is in the sufficient category. Abstrak: Kondisi fisik merupakan peranan yang penting dalam sepak bola karena dengan kondisi fisik yang baik maka akan lebih berpeluang untuk berprestasi. Penelitian ini bertujuan untuk mengetahui tingkat kondisi fisik pemain arema usia 16 tahun di Kota Malang tahun 2021. Metode yang digunakan dalam penelitian ini merupakan kuantitatif deskriptif yang bertujuan untuk memberikan gambaran nyata sesuai dengan yang ada di lapangan tentang kondisi fisik pemain sepak bola Arema Football Academy umur 16 tahun di Kota Malang tahun 2021. Hasil pada kekuatan otot tungkai memiliki rata-rata 44,24, kecepatan memiliki rata-rata 7,73, kelincahan memiliki rata-rata 9,91, kelentukan memiliki rata-rata 16,60, power otot tungkai memiliki rata-rata 55,88, daya tahan memiliki rata-rata 45,98. Dapat disimpulkan bahwa kekuatan (otot tungkai) masuk kategori cukup, kecepatan masuk kategori baik, kelincahan masuk kategori baik sekali, kelentukan masuk kategori baik sekali, power otot tungkai masuk kategori baik dan daya tahan masuk kategori baik. Dan pada kondisi fisik secara keseluruhan masuk dalam kategori cukup.

Steady exercise improves hand grip and leg muscle strength in hemodialysis patients

Sarcopenia due to chronic inflammation and biochemical disturbances in chronic kidney disease is severer and more prevalent in hemodialysis (HD) patients. We longitudinally evaluated the hand grip strength (HGS) and leg muscle strength (LMS) and evaluated the role of exercise in muscle strength in HD patients. We screened (January, n= 127) and followed up (June, n= 110 and December 2020, n= 104). HGS and LMS at single center by using digital hand and leg dynamometer. HGS (24.2 kg vs. 15.5 kg) and LMS (32.8 kg vs. 22.5 kg) were better in men (P<0.001 and P<0.001, respectively). Older patients ( ≥ 60 years) showed decreased LMS than others in women (P=0.01). Patients who performed steady home- or hospital-based exercise showed marginally higher HGS (23.1 kg vs. 19.8 kg, P=0.07) and significantly higher LMS (33.7 kg vs. 25.9 kg, P=0.004). Steady exercise improved LMS throughout the study period (30.3 kg vs. 33.2 kg from Jan to Jun 2020, P=0.004; 30.3 kg vs. 34.2 kg from Jan to Dec 2020, P=0.014). Multiple linear regression analysis proved steady exercise was independently associated with better HGS and LMS. Steady exercise showed greater impact on LMS in male patients with longer HD vintage (≥ 44 months) and on HGS in younger male patients with shorter HD vintage (< 44 months). Steady exercise was an important determinant of muscle strength in HD patients. We need to encourage patients to steadily perform regular home- or group-exercise before sarcopenia develops.

The effect of whey protein on malondialdehyde, aerobic capacity, and leg muscle explosive power in basketball athletes

Background: High-physical activity, including aerobic capacity and leg muscle explosive power, can cause stress oxidative and decrease the performance of athletes. Whey protein contains essential amino acids that were beneficial to decreased malondialdehyde (MDA) levels.Objective: To analyze the effect of whey protein on MDA level, aerobic capacity, and leg muscle explosive power in basketball athletes.Materials and Methods: Randomized controlled trial using pre- and post-test design was conducted on 12 male athletes aged 16-18 years at PPLOP Central Java Basketball Club. The treatment group received 30 grams of whey protein, and the control group received 30 grams of chocolate powder as a placebo for 28 days. MDA levels were measured through Elisa methods. Aerobic capacity was measured by 20 meters sprint. Leg muscle explosive power was measured by vertical jump. Data were analyzed by an independent t-test.Results: The mean MDA levels before intervention in the whey protein group were 182.36 (±59.05), and the mean after the intervention was 171.83 (±5.46). The mean before the aerobic intervention capacity was 36.95 (±5.84), and the mean after the intervention was 49.75 (±3.53). The mean leg muscle explosive was 83.50 (±21.58), and the mean after the intervention was 87.33 (±16.68). There were no effect of whey protein on MDA levels (p>0,05), aerobic capacity (p>0,05) and leg muscle explosive power (p>0,05).Conclusion: Whey protein for 28 days had no effect on MDA levels, aerobic capacity, and leg muscle explosive power

Hubungan Antara Umur, Jenis Kelamin, Indeks Massa Tubuh, Lama Latihan Dengan Daya Ledak Otot Tungkai Pada Atlet Taekwondo

Taekwondo is a martial sport that relies on kicks. For a taekwondo athlete to kick, it is necessary to support the explosive power of the leg muscles. This study aims to analyze the relationship between age, sex, body mass index, length of exercise with leg muscle explosive power in taekwondo athletes. Subjects and Methods: This analytic descriptive study used a cross sectional design. The subjects of the study were taekwondo members at Sangkuriang Sports Hall and Lions Gymnasium in Cimahi City, West Java Province, totaling 95 people. The dependent variable is leg muscle explosive power. The independent variables were age, gender, body mass index and duration of exercise. The instruments used are questionnaires and vertical jump measuring instruments. Data were analyzed bivariately using OR and chi square test. The explosive power of the limbs increased with increasing age (OR= 2.89; 95% CI= 1.23 to 5.77; p= 0.010) and duration of exercise (OR= 7.44; 95% CI= 2.96 to 18.67; p <0.001). Leg explosive power decreased with body mass index but not statistically significant (OR= 0.72; 95% CI= 0.30 to 1.18; p= 0.600). Gender increased limb explosive power, but not statistically significant (OR= 1.14; 95% CI= 0.51 to 2.56; p= 0.900). The explosive power of the limbs increases with age and duration of exercise. The relationship between limb explosive power and sex and body mass index was not statistically significant

HUBUNGAN DAYA LEDAK OTOT TUNGKAI DENGAN KEMAMPUAN SMASH PADA EKTRAKURIKULER BOLAVOLI DI SMP NEGERI 6 KOTA LUBUKLINGGAU

The problem in this study is that volleyball extracurricular students at SMP Negeri 6 Lubuklinggau City still have jumps and punches in the weak category, this is due to the weak power of the students' leg muscles which causes the smash to be performed less than optimal. The research objective was to determine whether there was a relationship between the explosive power of the leg muscles and the smash ability of volleyball players at SMP Negeri 6 Lubuklinggau City. The variables of this study were the leg muscle explosive power as the independent variable, and the smash ability was the dependent variable. This type of research is correlational research. This research was conducted on 14 male volleyball extracurricular students at SMP Negeri 6 Lubuklinggau City. The instrument for collecting the explosive power of the leg muscles used the vertical jump test and the smash used the smash ability test. The data analysis technique used the product moment correlation formula. The results of the study showed that there was a relationship between the leg muscle explosive power (x) and the volleyball smash ability (y), the calculation result was rxy = 0.55 from this value can be categorized as moderate. The results stated that there was a significant relationship between the explosive power of leg muscles on the smash ability of volleyball extracurricular students at SMP Negeri 6 Lubuklinggau City, this was stated based on hypothesis testing where t hit = 2.280 > t tab = 2.178.

How Fit Are Special Operations Police Officers? A Comparison With Elite Athletes From Olympic Disciplines

The diverse tasks of special operations police (SOP) units place high physical demands on every officer. Being fit for duty requires a wide range of motor abilities which must be trained regularly and in a structured manner. But SOP operators have to plan and manage large proportions of their training alone, which makes it difficult to control. Therefore, this study aimed to highlight strengths and deficits of the SOP operators' fitness by comparing them to elite athletes, and to define future training goals. Retrospective data of 189 male SOP operators were used, who completed several isometric strength tests, a graded exercise test to determine maximal oxygen uptake, and countermovement jumps to determine leg muscle power. On the basis of a literature search, performance data were then compared to a total of 3,028 elite male athletes from 36 Summer Olympic disciplines. Pooled means and standard deviations were calculated for each discipline and effect sizes were used to analyze their similarities and differences to the SOP unit. On average, SOP operators were taller, heavier, and stronger than elite athletes. But both the ability to convert this strength into explosive movement and aerobic power was significantly less developed. From this point of view, SOP operators should consider polarized endurance training to work efficiently on improving aerobic performance. In addition, regular plyometric training seems necessary to improve leg muscle power and agility.