Removal of Acid Hematin-Type Pigments from Sections; Efficacy of Alcoholic Picric Acid Solution

1969 ◽  
Vol 44 (6) ◽  
pp. 279-281 ◽  
Author(s):  
Faye Sweat Waldrop ◽  
Holde Puchtler ◽  
Mary S. Terry
Keyword(s):  
Author(s):  
W. R. G. Atkins

The fifth paper of this series (1930) was the author's last, after which the work was handed over to L. H. N. Cooper. It was resumed by the author in 1948 to compare the early years with the post-war, since the determinations had lapsed. Measurements of the extinction coefficient of the water were also resumed, and it was desirable to obtain the chemical data at precisely the same time.The phosphate results are, as before, expressed as P2O5 in mg./m.3, since for crop calculations this old unit is convenient; to convert to mg. P, which is more rational, multiply by 0437; to bring to milligram atoms multiply by 0–0141. The analyses have not been corrected for salt error, since the early results were not^ and the factor for this correction has not as yet been agreed upon.The silicate results are given as mg./m.3, of SiO2, but though the standard used in Plymouth was checked for me by Dr E. J. King in Canada and agreed with his (King & Lucas, 1928), yet a different value had to be assigned to the silica factor of the picric acid solution. All values have accordingly been multiplied by I.44.The phosphate results for E1, surface, from 11 February 1948 to 1 February 1949 are shown in Fig. plotted against the dates.


Author(s):  
E. P. Abrahamson II ◽  
M. W. Dumais

In a transmission microscopy study of iron and dilute iron base alloys, it was determined that it is possible to preserve specimens for extended periods of time. Our specimens were prepunched from 5 to 8 mil sheet to microscope size and annealed for several hours at 700°C. They were then thinned in a glacial acetic-12 percent perchloric acid solution using 10 volts and 20 milliamperes, at a temperature of 8 to 14°C.It was noted that by the use of a cold stage, the same specimen can be observed for periods up to one week without excess contamination. When removal of the specimen from the column becomes necessary, it was observed that a specimen may be kept for later observation in 1,2 dichloroethene or methanol for periods in excess of two weeks.


Author(s):  
Larry F. Lemanski ◽  
Eldridge M. Bertke ◽  
J. T. Justus

A recessive mutation has been recently described in the Mexican Axolotl, Ambystoma mexicanum; in which the heart forms structurally, but does not contract (Humphrey, 1968. Anat. Rec. 160:475). In this study, the fine structure of myocardial cells from normal (+/+; +/c) and cardiac lethal mutant (c/c) embryos at Harrison's stage 40 was compared. The hearts were fixed in a 0.1 M phosphate buffered formaldehyde-glutaraldehyde-picric acid-styphnic acid mixture and were post fixed in 0.1 M s-collidine buffered 1% osmium tetroxide. A detailed study of heart development in normal and mutant embryos from stages 25-46 will be described elsewhere.


Author(s):  
Michio Ashida ◽  
Yasukiyo Ueda

An anodic oxide film is formed on aluminum in an acidic elecrolyte during anodizing. The structure of the oxide film was observed directly by carbon replica method(l) and ultra-thin sectioning method(2). The oxide film consists of barrier layer and porous layer constructed with fine hexagonal cellular structure. The diameter of micro pores and the thickness of barrier layer depend on the applying voltage and electrolyte. Because the dimension of the pore corresponds to that of colloidal particles, many metals deposit in the pores. When the oxide film is treated as anode in emulsion of polyelectrolyte, the emulsion particles migrate onto the film and deposit on it. We investigated the behavior of the emulsion particles during electrodeposition.Aluminum foils (99.3%) were anodized in either 0.25M oxalic acid solution at 30°C or 3M sulfuric acid solution at 20°C. After washing with distilled water, the oxide films used as anode were coated with emulsion particles by applying voltage of 200V and then they were cured at 190°C for 30 minutes.


1967 ◽  
Vol 18 (01/02) ◽  
pp. 057-065 ◽  
Author(s):  
G Zbinden

SummaryIntravenous injection of 0.5% lauric acid solution into rabbits caused moderate to marked thrombocytopenia. With small doses (2.5 mg/kg) this thrombocyte decrease was reversible and microscopically demonstrable thrombosis in the lungs was only seen or suspected in a small number of rabbits 10 to 30 min after lauric acid injection. High doses were followed by partly reversible thrombocytopenia and by moderate to marked, sometimes lethal, thrombosis in the lungs still demonstrable 24 hrs after injection. Repeated administration of small doses of lauric acid did not lead to a depletion of the circulating thrombocytes. Thrombocytopenic response, however, appeared to be less pronounced after the second and subsequent injections. Studies with Cr51-labeled platelets indicate that during the reversible thrombocytopenia following a small intravenous dose of lauric acid platelets are retained in various organs, particularly the lungs.


2011 ◽  
Vol 3 (11) ◽  
pp. 456-458
Author(s):  
D. Jencyline Navarani ◽  
◽  
P. Selvarajan P. Selvarajan

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