Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids

Campylobacter jejuni is a major bacterial cause of human diarrheal diseases worldwide. Despite its sensitivity to environmental stresses, C. jejuni ubiquitously distributes throughout poultry production chains. Biofilm formation mediated by quorum sensing is suggested to be critical to the survival of C. jejuni in agroecosystem. C. jejuni possesses LuxS, the enzyme involved in the production of autoinducer-2 (AI-2) signaling molecules. In this study, two fatty acids, namely decanoic acid and lauric acid, were identified to be effective in inhibiting AI-2 activity of C. jejuni. Both decanoic acid and lauric acid at 100 ppm inhibited ∼90% AI-2 activity (P < 0.05) of C. jejuni without bacterial inactivation. The biofilm biomass of two C. jejuni strains was reduced by 10–50% (P < 0.05) after treatment by both fatty acids, while increased biofilm formation was observed for one C. jejuni strain. In addition, both fatty acids effectively reduced the motility of all tested C. jejuni strains. These findings can aid in developing alternative C. jejuni control strategies in agri-food and clinical settings.

Effect of dietary supplementation of lauric acid on growth performance, antioxidative capacity, intestinal development and gut microbiota on black sea bream (Acanthopagrus schlegelii)

A feeding trial of eight weeks was conducted to examine the influence of food supplementation with lauric acid (LA) on Acanthopagrus schlegelii (juvenile black sea bream). A 24 percent fish meal baseline diet was created, while the other two diets were generated with dietary supplementation of graded points of LA at 0.1 percent and 0.8 percent, respectively. Each diet was given a triplicate tank with 20 fish weighing 6.22 ± 0.19 g. In comparison with the control group, the weight gain rate, growth rate, as well as feed efficiency of fish fed of 0.1 percent diet of LA were considerably (P < 0.05) greater. The total body and dorsal muscle proximate compositions did not change significantly between groups (P > 0.05). Triglyceride (TG) content was considerably (P < 0.05) greater in the LA-supplemented meals eating group in comparison with the control group. In the group eating LA-supplemented meals, the height of villus and the number of goblet cells/villus were considerably (P < 0.05) larger. The microbial makeup of the gut was also studied. The differences in phyla, class, and family level were not statistically significant (P > 0.05). Firmicutes in the phylum, Betaproteobacteri, Gammaproteobacteria, and Clostridia in the class, and Clostridiaceae in the family were all substantially increased with higher levels of LA supplementation (P < 0.05). According to the findings of this study, an LA-supplemented diet improves fish development, antioxidative capability, gut microbiota and intestinal health.

TESTING THE EFFECTIVENESS OF ORGANIC ACIDS AS A PRESERVATIVE OF ANIMAL FEED INGREDIENTS

This research was conducted in the Laboratory of Feed Industry Technology, Faculty of Animal Husbandry, Bogor Agricultural University, Bogor. The purpose of this study was to determine the effectiveness of using organic acids as preservatives in feed ingredients in the form of pollard and wheat bran based on physical parameters. This study used a completely randomized design with 2 factorials in the form of feed type and organic acid type with 3 replications, samples were stored for 6 weeks and then data were collected in the form of moisture content, pile angle, pile density and specific gravity. The results showed that organic acids affected the moisture content but had no effect on pile density, pile angle and specific gravity. The type of feed affects the value of moisture content, pile angle, pile density and specific gravity. Based on the results of the effectiveness index test, it is known that the total yield value on pollard with 1% lauric acid is 0.64, sorbic acid is 0.35 and propionic acid is 0.77, while wheat bran with lauric acid is 1.07 at -1 sorbic acid. .39 and in propionic acid 2.64. The conclusion of this research is that propionic acid can be used as a preservative for pollard and wheat bran

Formulasi Sabun Cair Dengan Kombinasi Minyak Kelapa Murni (VCO) dan Ekstrak Daun Miana (Coleus atropurpureus)

Saponification between alkaline salt and virgin coconut oil (VCO) will produce liquid soap. This is because the use of virgin coconut oil (VCO) in saponification is known to be very good because of the presence of lauric acid which has clean power and functions as an antimicrobial. The addition of miana leaf extract (Coleus atropurpureus) in the manufacture of liquid soap because it contains flavonoids, tannins, saponins, essential oils which also function as moisturizers and antioxidants. The purpose of this study was to determine the effectiveness of the best combination of pure coconut oil (VCO) liquid soap with variations in the concentration of miana leaf extract (Coleus atropurpureus) in reducing free radicals in the form of bacteria. In this study, a laboratory experimental method was used with the addition of miana leaf extract. A= 0% (w/w), B= 10% (w/w), C= 15% (w/w),) in 100 mL total virgin coconut oil (VCO) three times. The test parameters were in the form of a bacteriological test using the Total Plate Number (ALT) method. From the results of the study, it was found that the formulation of pure coconut oil (VCO) liquid soap with various concentrations of miana leaf extract (Coleus atropurpureus) could reduce free radicals such as bacteria.

Alterations in health-related fatty acids in buffalo milk after processing to traditional dairy products

Milk provides some beneficial fatty acids which in dairy processing are subjected to pasteurization and fermentation. With the aim to assess such changes, aliquot parts of milk from 12 buffaloes were pooled and processed to germinated yoghurt and brined cheese, and to non-germinated curd – the respective samples of raw and dairy material subjected to lipid analysis. The results show that in cheese positive and negative changes are generally balanced, rumenic acid decreasing and other CLAs altered but not total CLA and PUFA; omega ratio and atherogenicity index worsened to little extent, due to adverse change in n-3, myristic and lauric acid. In yoghurt and curd CLA dramatically decreased, excluding rumenic acid; but vaccenic acid increased, though total trans isomers decreased; the worsened n-6/n-3 ratio and atherogenicity index is mostly because of the adverse effect on PUFAn-3 but also on myristic and lauric acid. In all products SFA and MUFA did not change, including palmitic, stearic, and oleic acid. It can be concluded that the decrease of CLA in yoghurt and curd is partially compensated by the increase in the vaccenic acid, while cheese making altered individual isomers but not groups of beneficial acids.