DCH18C6 using strontium extraction selectivity investigation and sequential soil fractionation study

85Sr as radioindicator has been applied to strontium separation selectivity study using cisdicyclohexano-18-crown-6 (DCH18C6) as extraction agent and picric acid as counter ion with the aim to contribute to the development of a rapid method of strontium extraction. The same radioindicator has been used for strontium fractionation study in chosen soils by application of Tessier- and BCR sequential extraction procedures. Experimental results have shown, that the mentioned extraction system is applicable for IDA determination of Sr2+ in ∼1000-fold abundance of Ca2+, ∼1000-fold abundance and Mg2+, ∼10-fold abundance of K+ and ∼0.2-fold abundance of Ba2+. For the soil samples - chernozems developed on loess - from the near vicinity of NPP Jaslovské Bohunice the exchangeable strontium fraction reached as much as 50 – 60 %.

Surgico-pathological analysis of testicular lesions

The primary objective of our study was to identify the different types of histopathological lesions of testes and epididymis occurring in patients admitted in a tertiary care hospital, the objective was also to analyze the spectrum of testicular lesions, our aim was to evaluate the prevalence of testicular malignancies and characterize the tumor based on their histo-morphological features. This was a prospective study of 2 years (October 2018 to October 2020) aggregating a total of 52 cases. The surgical specimens were referred to the pathology department for histopathological analysis. Adetailed preoperative history of every case with regards to age, presenting symptoms, and signs along with personal history were obtained.Also, relevant investigations like serum tumor markers and Ultrasonography findings were analyzed. Postoperatively, the tissue was sent to the histopathology department was studied by proper fixation, gross examination, adequate sampling, and paraffin sections of the same.10% formalin was used to fix the specimen. Some resources suggest the use of Bouin solution for the fixation of testis biopsy specimens. However, the picric acid in the Bouin solution presents challenges for safe handling and disposal due to its explosive potential.Hence, given the available resources in our laboratory setting, formalin was the preferred choice of fixative. We ensured that the specimens came in adequate amount of formalin from the OT with correct labeling.We excluded the autolyzed specimens. The data was noted and quantitative analysis was done.The study was further compared with other previously reported studies. 52 cases were studied and majority of the cases were reported in the age group of 31-40yrs. 65.4% cases were non neoplastic, with testicular torsion being the commonest lesion. Amongthe 23.1% neoplastic cases that were reported, mixed germ cell tumor was the commonest (75%). Non neoplastic lesions are more common than neoplastic lesions. Torsion testis was the commonest indication for orchidectomy in younger age group. Despite newer techniques in imaging and tumor marker assays the diagnosis of testicular lesions is primarily dependent upon histopathological examination.

Structural Features of Glands in the Sphincter Zones of the Large Intestine of an Adult

The aim is to study macro- and microscopic structure, as well as the cellular composition of the glands of the sphincter zones of large intestine of adults of different age groups.Material and methods. On autopsy material obtained from 30 people, without signs of pathology of the digestive tract of three age groups: 20–29 years, 50–59 years, 90–99 years, the structure of the glandular apparatus of the sphincter zones was studied. The areas of the Gerlach flap, Girsch sphincters, Payr–Strauss, Bally, O'bern–Pirogov–Moutier were considered. Quantitative morphometry was performed on histological preparations stained with methylene blue, followed by fixation in a saturated solution of ammonium molybdenum (picric acid), hematoxylin-eosin, picrofuchsin according to Van Gieson. Methods of parametric statistics based on the Statistica 6.0 program were used for statistical data processing.Results. The analysis of the number, size and cellular composition of the glands of the sphincter zones of the large intestine revealed an increase in both the number of glands and their size in all age groups compared to the proximally adjacent areas of the intestine, on average by 1.3–1.5 times. In a similar range, individual indicators of the number of epithelial cells in the glands of the sphincter zones of the colon increased. At the same time, the cellular composition of the glands of the sphincter zones and adjacent areas of the intestinal wall was similar to neighboring areas, with predominant goblet-shaped epithelial cells (52.9–54.2% of cells on the longitudinal section of the gland) and the presence of absorption cells (29.9–31.2%), undifferentiated – 11.9–13.2% and argyrophilic endocrinocytes – 1.4–5.3%.Conclusion. Against the background of narrowing of the lumen of the large intestine in the area of the sphincters and changes in the nature of the mucous membrane, there is an increase in the size and density of the localization of glands in all age groups. This confirms the thesis about the formation of a protective barrier that provides a local adaptive potential of this area of the intestinal wall, against the background of increased mechanical effects of intestinal masses.