Aldehyde Fuchsin Staining, Direct Or After Oxidation: Problems And Remedies, With Special Reference To Human Pancreatic B Cells, Pituitaries, And Elastic Fibers

1978 ◽  
Vol 53 (3) ◽  
pp. 141-154 ◽  
Author(s):  
Robert W. Mowry
Keyword(s):  
B Cells ◽  
Special Reference ◽  
Elastic Fibers ◽  
Aldehyde Fuchsin ◽  
Pancreatic B Cells

scholarly journals Staining properties of aldehyde fuchsin analogs.

1979 ◽  
Vol 27 (3) ◽  
pp. 782-787 ◽  
Author(s):  
T S Buehner ◽  
G S Nettleton ◽  
J B Longley
Keyword(s):  
B Cells ◽  
Reaction Mechanisms ◽  
Elastic Tissue ◽  
Aldehyde Fuchsin ◽  
Pancreatic B Cells

This investigation was designed to clarify the role of the aldehyde component of aldehyde fuchsin in its staining reactions. Several aldehyde fuchsin analogs were prepared by using different aldehydes. The staining quality of these analogs and pararosaniline-HCl was compared with that of aldehyde fuchsin prepared with paraldehyde in the usual way. The major findings of this investigation include: 1) Aldehyde fuchsin staining of nonoxidized pancreatic B cells requires a stain prepared with either paraldehyde or acetaldehyde. 2) An aldehyde moiety is required for aldehyde fuchsin staining of strong tissue anions. 3) Staining of elastic tissue with aldehyde fuchsin analogs resembles staining of strong tissue anions more than staining of nonoxidized pancreatic B cells. Possible reaction mechanisms of aldehyde fuchsin with tissue substrates are discussed.

scholarly journals The role of paraldehyde in the rapid preparation of aldehyde fuchsin.

1982 ◽  
Vol 30 (2) ◽  
pp. 175-178 ◽  
Author(s):  
G S Nettleton
Keyword(s):  
B Cells ◽  
Room Temperature ◽  
Rapid Preparation ◽  
Selective Staining ◽  
Aldehyde Fuchsin ◽  
Accelerated Ripening ◽  
Pancreatic B Cells ◽  
Fold Excess ◽  
Staining Time

Preparation of aldehyde fuchsin normally requires ripening for 3 to 5 days. By using a 5-fold excess of paraldehyde a fully potent aldehyde fuchsin can be prepared in 24 hr at room temperature. Aldehyde fuchsin prepared by both normal and accelerated ripening afforded comparable results, including selective staining of unoxidized pancreatic B cells. Dried aldehyde fuchsin prepared form pararosaniline and reconstituted in acid alcohol has spectrophotometric properties different form the ripened strain. Reconstituted aldehyde fuchsin stains unoxidized B cells adequately only if staining time is extended. Excess paraldehyde added to reconstituted aldehyde fuchsin retards decomposition but does not produce a normal stain by spectrophotometric standards. Warming of aldehyde fuchsin solutions to accelerate ripening has been shown to produce deleterious effects and should be avoided.

scholarly journals Effects of fuchsin variants in aldehyde fuchsin staining.

1980 ◽  
Vol 28 (7) ◽  
pp. 683-688 ◽  
Author(s):  
S J Lichtenstein ◽  
G S Nettleton
Keyword(s):  
Schiff Base ◽  
B Cells ◽  
Chemical Structure ◽  
Condensation Product ◽  
Staining Reaction ◽  
Aldehyde Fuchsin ◽  
Base Derivative ◽  
Pancreatic B Cells ◽  
Base Structure

Aldehyde fuchsin stains pancreatic B cell granules, hypophyseal basophils, goblet cell mucins, gastric chief cells, hyaline cartilage, and elastica. Neither the chemical structure of aldehyde fuchsin nor its staining mechanism is known. This study was undertaken to clarify the role of the fuchsin component of aldehyde fuchsin in its staining reaction. The major findings of this investigation include: 1) single N-methylation of the fuchsin molecule abolishes staining of unoxidized pancreatic B cells, although it does not prevent reaction of fuchsin with paraldehyde; 2) aldehyde fuchsin is probably a Schiff base condensation product of pararosaniline and acetaldehyde; 3) a Schiff base structure alone cannot account for aldehyde fuchsin staining of unoxidized pancreatic B cells; 4) a fully potent aldehyde fuchsin is possibly a Tris-Schiff base derivative of pararosaniline.

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