Histotopographical study of human periocular elastic fibers using aldehyde-fuchsin staining with special reference to the sleeve and pulley system for extraocular rectus muscles

The presence of "elastic" fibers in addition to collagen in the mesogloea of the column wall of Pachycerianthus has been demonstrated by staining with spirit blue or aldehyde – fuchsin after oxidation with potassium permanganate.

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SOME HISTOCHEMICAL CHARACTERISTICS OF TISSUES IN LARVAE OF CTENICERA DESTRUCTOR (BROWN) (COLEOPTERA, ELATERIDAE), WITH SPECIAL REFERENCE TO CUTANEOUS SENSILLA

Canadian Journal of Zoology ◽

10.1139/z62-068 ◽

1962 ◽

Vol 40 (5) ◽

pp. 733-746 ◽

Cited By ~ 1

Author(s):

R. Y. Zacharuk

Keyword(s):

Special Reference ◽

Potassium Permanganate ◽

Protein Complex ◽

Recurrent Nerve ◽

Efferent System ◽

Sh Groups ◽

Sensory Axons ◽

Aldehyde Fuchsin ◽

Cuticular Structures ◽

Staining Characteristic

The sensory axons from the cutaneous sensilla and some of those in the recurrent nerve stain strongly with S-specific stains. The axons of the efferent system and those from the ocelli lack this staining characteristic. This difference among axons possibly is related to the origin of their precursors in the ontogenetic sequence.Some of the metabolites involved in the synthesis of cuticular structures are demonstrated and discussed. The following sequence in the synthesis of the cuticula is suggested: glycogens → more complex, diastase-fast polysaccharides → chitin → a carbohydrate–protein complex containing SS groups → a complex (procuticle) with potential SH groups → a complex (exocuticle) with bound S and a high content of tyrosine and other phenols. The sequence in the synthesis of the cuticular nerve sheaths appears basically similar. The last step was not evident in tonofibrillae, and the last two steps were not evident in the subcuticular sheaths or axoplasm of reactive axons.The mechanisms of the histochemical reactions are discussed, with particular reference to staining with aldehyde-fuchsin after oxidation with potassium permanganate. This method may serve to differentiate histologically certain afferent from efferent axons in insect nervous systems.

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Ultrastructure and Staining of Developing Elastic Tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065274 ◽

1971 ◽

Vol 29 ◽

pp. 244-245

Author(s):

E. N. Albert

Keyword(s):

Fine Structure ◽

Phosphotungstic Acid ◽

Staining Method ◽

Rat Aorta ◽

Uranyl Acetate ◽

The Other ◽

Elastic Fibers ◽

Elastic Tissue ◽

Lead Citrate ◽

New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

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