Immunohistochemical characterization of novel monoclonal antibodies against the N-terminus of amyloid β-peptide

Amyloid ◽  
2013 ◽  
Vol 20 (3) ◽  
pp. 179-187 ◽  
Author(s):  
Nicolaas A. Verwey ◽  
Jeroen J.M. Hoozemans ◽  
Carsten Korth ◽  
Marloes R. van Royen ◽  
Ingrid Prikulis ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
N Terminus

scholarly journals Characterization of four new monoclonal antibodies against the distal N-terminal region of PrPc

2014 ◽  
Author(s):  
Alessandro Didonna ◽  
Anja Colja Venturini ◽  
Katrina Hartman ◽  
Tanja Vranac ◽  
Vladka Curin Serbec ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Prion Protein ◽  
Biochemical Characterization ◽  
Prion Diseases ◽  
Physiological Role ◽  
Prion Infection ◽  
Promising Tool ◽  
C Terminus ◽  
N Terminus

Prion diseases are a group of fatal neurodegenerative disorders that affect humans and animals. They are characterized by the accumulation in the central nervous system of a pathological form of the host-encoded prion protein (PrPC). The prion protein is a membrane glycoprotein that consists of two domains: a globular, structured C-terminus and an unstructured N-terminus. The N-terminal part of the protein is involved in different functions in both health and disease. In the present work we discuss the production and biochemical characterization of a panel of four monoclonal antibodies (mAbs) against the distal N-terminus of PrPC using a well-established methodology based on the immunization of Prnp0/0 mice. Additionally, we show their ability to block prion (PrPSc) replication at nanomolar concentrations in a cell culture model of prion infection. These mAbs represent a promising tool for prion diagnostics and for studying the physiological role of the N-terminal domain of PrPC.

Quenched hydrogen/deuterium exchange NMR characterization of amyloid-β peptide aggregates formed in the presence of Cu2+or Zn2+

FEBS Journal ◽  
2009 ◽  
Vol 276 (15) ◽  
pp. 4051-4060 ◽  
Author(s):  
Anders Olofsson ◽  
Malin Lindhagen-Persson ◽  
Monika Vestling ◽  
A. Elisabeth Sauer-Eriksson ◽  
Anders Öhman
Keyword(s):  
Amyloid Β ◽  
Deuterium Exchange ◽  
Amyloid Β Peptide ◽  
Hydrogen Deuterium Exchange ◽  
Nmr Characterization ◽  
Hydrogen Deuterium

scholarly journals Generation of the Amyloid-β Peptide N Terminus inSaccharomyces cerevisiaeExpressing Human Alzheimer's Amyloid-β Precursor Protein

1999 ◽  
Vol 274 (48) ◽  
pp. 33843-33846 ◽  
Author(s):  
Jeffrey P. Greenfield ◽  
Huaxi Xu ◽  
Paul Greengard ◽  
Sam Gandy ◽  
Mary Seeger
Keyword(s):  
Amyloid Β ◽  
Precursor Protein ◽  
Amyloid Β Peptide ◽  
N Terminus

scholarly journals Characterization of the Binding of Amyloid-β Peptide to Cell Culture-Derived Native Apolipoprotein E2, E3, and E4 Isoforms and to Isoforms from Human Plasma

2002 ◽  
Vol 68 (2) ◽  
pp. 721-725 ◽  
Author(s):  
Dun-Sheng Yang ◽  
Jonathan D. Smith ◽  
Zhongmin Zhou ◽  
Samuel E. Gandy ◽  
Ralph N. Martins
Keyword(s):  
Cell Culture ◽  
Human Plasma ◽  
Amyloid Β ◽  
Amyloid Β Peptide

Identification and Nanomechanical Characterization of the HIV Tat‐Amyloid β Peptide Multifibrillar Structures

2020 ◽  
Vol 26 (43) ◽  
pp. 9449-9453
Author(s):  
Qiying Feng ◽  
Yue Hong ◽  
Naga Pradeep Nidamanuri ◽  
Chuanxu Yang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Hiv Tat

scholarly journals Characterization of stable complexes involving apolipoprotein E and the amyloid β peptide in Alzheimer's disease brain

Neuron ◽  
1995 ◽  
Vol 15 (1) ◽  
pp. 219-228 ◽  
Author(s):  
Jan Näslund ◽  
Johan Thyberg ◽  
Lars O. Tjernberg ◽  
Christer Wernstedt ◽  
Anders R. Karlström ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Apolipoprotein E ◽  
Amyloid Β ◽  
Amyloid Β Peptide
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